Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 604 results for: abo.2

Showing: 321 - 340 of 604 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...mean you have one copy of the gene; an RQ value of 2 means you have two copies, and so on and so forth....quantitative PCR. Expert Rev Mol Diagn. 2005 Mar;5(2):209-19. doi: 10.1586/14737159.5.2.209. PMID: 15833050...controls must be run in triplicate.  The nice thing about qPCR is that since it measures relative quantification...number, albeit within a range of RQ values as noted above.   In both applications, results can be affected...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...estimate transformation efficiency (Figure 2).    Figure 2: Example of a serial dilution to estimate...First, you’ll need to collect specific information about your library: the size of your library (How many...with Addgene. If you have any specific questions about designing your library amplification protocol, feel...

  3. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ... of permeabilization and membrane integrity (Fig. 2). For example, saponin interacts with cholesterol ... at too high a concentration.      Figure 2: Examples of different permeabilization strategies...and detergents.  Organic Solvents  As mentioned above, organic solvents can be used to simultaneously ...permeabilizing with saponin. However, if you are not worried about washing away your target (for example, some cytoskeletal...

  4. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...from GFP and the mutation definitions found in Table 2 may not apply. Table 1: The specific mutations comprising..., V163A EBFP F64L, S65T, Y66H, Y145F   Table 2: Functional role of specific mutations in GFP derivatives... activity, and much more. Read on to learn more about GFP, how scientists have evolved this versatile ...effects of alterations to this activity. Learn more about optogenetics at OpenOptogenetics and find optogeneic...

  5. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or...for different cell types (known as tropism). Figure 2: Gamma-retroviral plasmids. Created with BioRender.com...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...gamma-retroviral vectors that can be safely used in laboratory settings. These engineered gamma-retroviral vectors...of plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...increased safety when producing viral vectors in a laboratory setting. Figure 1: Wild-type gamma-retrovirus...packaging methods . The three plasmids described above (envelope, packaging, and transfer) are co-transfected...

  6. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Indicators GEVIs can be divided up structurally into 2 main groups: voltage sensitive domains and Rhodopsin...depending on your experimental system.   Figure 2: General overview of chemigenetic tools. A genetically...especially essential. If you want to learn more about how viruses can be used for chemigenetics, biosensors...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...of ~75% in response to the magnetic field (Figure 2); they named this variant MagLOV. Andrew was thrilled...didn’t need any added cofactors.      Figure 2: Fluorescence intensity and ΔF/F of MagLOV, a variant...our tissues unimpeded. Andrew and Maria dreamed about the possibilities of a magnetoresponsive fluorescent...of improved response. Then, just when Andrew was about to suggest she deprioritize the project, Maria’s...with a strong commitment to exploration and collaboration. Andrew is a physicist with a background in ...potential applications.  Andrew is a frequent collaborator and is happy to give advice and support for ...interested in working on these questions or collaborating, the York Lab is eager to hear from you. Find...

  9. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    ...mutations. Listen to Our Segment on CRISPR X Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal...this new way to learn about plasmid technologies. If you have any comments about the Hot Plasmids Podcast...with these efforts, you’ll find new ways to learn about science careers, lab protocols, and, of course, ...each one, plug in some headphones to learn more about the latest and greatest plasmids through this podcast...

  10. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...with neuron-specific expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church...ubiquitous ones such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression... to target individual genes can be relatively laborious because multiple plasmids/viruses are needed to...screening strategies Although the tools described above make CRISPRa more accessible to neuroscientists,...vitro and in vivo applications, the tools described above make CRISPRa in the brain relatively easy and accessible... CNS using CRISPRa as a member of the Gelbard Laboratory at URMC.     References Liu, Yanxia, et al. "...

  11. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...connectivity to other data sources used in research; (2) connectivity to other tools used in research; and...by making it easy to get data out of the ELN; and (2) facilitate inter-operability with other research ...RSpace is integrated with most of the tools noted above, and has a modern API. Integrations with additional...into RSpace. The kind of informal conversations about research that can take place in Slack normally never...RSpace documents to external data sources (described above) further enhances the value the repository integrations...Minisymposium on Reproducibility Read all blog posts about scientific sharing Resources on Addgene.org Find...

  12. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...diffusion test or Kirby-Bauer susceptibility test (Figure 2), and requires at least 18-24 hours to obtain results...." London: Wellcome Trust & HM Government (2016). 2. CDC Information Pages on Antibiotics 3. WHO Antimicrobial...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...CDC. Antibiotic resistance can be scary to think about, but we can all do our part to learn all we can,...drug resistance. Additionally, she is passionate about health equity, science communication, and public...

  13. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

    Type
    Blog Post
    ... Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “Cell type-specific manipulation... Cepko. Listen to the podcast below to hear all about the research being conducted in Professor Cepko'...and mentoring styles. If you'd like to learn more about Professor Cepko's recent work on developing GFP-...the time to talk to us and look forward to hear about more exciting discoveries from her lab. Special ...

  14. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    ...green squares, purple circles and blue triangles. 2) An antibody immobilized on a bead binds specifically...protein G, a novel IgG-binding reagent. J Immunol 133(2):969-974. Hjelm H, Hjelm K, Sjöquist J (1972) Protein...proteins or nucleic acids. Read on to learn more about this versatile technique.  Immunoprecipitation ...proteins in an IP are not denatured (learn more about antibodies here!) When using Protein A/G to immobilize...eluted by boiling the beads in SDS. (To learn more about Western blotting check out our blog post The Basics...

  15. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    ..., visit Vector Database. Step 2: Insert name  Include information about the insert in your plasmid name... just by reading the name. For more information about plasmid features and tips you can use in the lab...

  16. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...antibiotic selection. Essentially, this gene is a 2-in-1 providing both bacterial and eukaryotic selection...Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https://doi.org/10.1093/mutage/gei016I ...therefore prevent cell death. One of the cool things about amp is that it's exclusively toxic to dividing cells...antibiotic selection. If you feel like multiple of the above antibiotics would work for you, they likely do! ...

  17. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    ...expressed as a fusion with your favorite protein and (2) a synthetic fluorophore-contianing “ligand” that ...cell 58.4 (2015): 644-659. PubMed PMID: 26000849. 2. Grimm, Jonathan B., et al. "A general method to improve... the frontiers of biological imaging.  *Worried about your budget? Email [email protected] Resources on the Addgene Blog Learn About Other Protein Tags Catch Up on Your Fluorescent ...

  18. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...retain their individual activities when separated (2), and that DBD and TAs from different systems could... Enzymol. 1983;100:293-308. PubMed PMID: 6312261. 2. Keegan, L., Gill, G., and Ptashne, M. Separation ... Plasmids Read our Blog Post About Yeast Vectors Read our Blog Post About Tagging Yeast Genes ...

  19. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...of these neurons via the channel Channelrhodopsin 2 (ChR2) reduced glutamate transmission from the mPFC...deliver Cre-dependent expression of  Channelrhodopsin 2 (ChR2), a modified receptor protein that activates...distant regions. They also don’t give any clues about the types of neurons involved or whether their involvement... Viral Vectors Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  20. PEI Calculator for Planning AAV Packaging Transfections

    Type
    Blog Post
    ...pHelper: 1 RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue culture dish you...packaging experiments. Let us know what you think about the calculator in the comments section below. References...
Showing: 321 - 340 of 604 results