We narrowed to 643 results for: car

... to try this at home in their own lab: be very careful with the type IIS restriction endonuclease of choice... PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision Cloning...

...covered in a future post in this series). Members carry on back-channel or side conversations within the...and suggestions. Consider offering rewards (gift card, afternoon off, etc.) Act on information to make...

...plasmids each carrying a single gene with a defined mutation or gene fusion. Plasmids carrying the corresponding...

...Essen                      University of North Carolina at Chapel Hill University of Illinois Chicago                     ...Consortium collection enables research on solute carriers (SLCs) for medical research Recently, the Michael...

... that your request is ready to order. An “Add to Cart” button will appear with the request in your Addgene... be charged once you have added the prep to your cart and checked out.  Prices for Packaged on Request...

...genomic loci of interest and on either side of the cargo (ex: fluorescent protein gene) on the donor vector...sequence. Homology arms that flank the donor plasmid cargo are complementary to either side of the double-stranded...

..., R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4, indicating that ...and thus nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity...

...dendritic cells. These genetic tools were pivotal in carrying out my studies, and getting them from collaborators...has since studied and worked around the world, carrying out her undergraduate work at the University of...

...data found in full-length publications. As early career researchers, we wondered how we could make the ... blogger Girija Goyal! Girija Goyal is an early career researcher and cofounded ReFigure with Joe Akin...

.... Writing is the cornerstone of any scientist’s career. We use writing to communicate our findings and...consisted of graduate students, post-grads, and early career scientists. Over the course of the class, the moderator...

... stone into the next professional stage of your career by connecting you with people of similar interests... research question that people (especially you!) care about. Have you critically evaluated your plan...

...wall synthesis Bactericidal 100-200 ug/mL Carbenicillin beta-lactam Inhibits cell wall synthesis Bactericidal...optimal efficiency. Beware of satellite colonies! Carbenicillin is more stable than Ampicillin and can be used...

...Author: James Atmos (2). Many CRISPR/Cas moieties carry out these functions using slightly different Cas... Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. Science. 2013 Jan;  ...

...pigments primarily found in plants in the order Caryophyllales, such as cacti and beets. Betanin belongs to... Biology Department at the University of North Carolina at Chapel Hill. He has a passion for microscopy...

...LTRs). After recombination, one resulting plasmid carries the transgene of interest (aka the viral genome...picking the smaller colonies which are more likely to carry the full plasmid. Chakiath & Esposito (2018) noticed...

... new particles. As a bonus, this increases the carrying capacity of the viral vector, increasing both ... possible, just to increase the viral vector’s carrying capacity. In order to allow the viral vectors ...

...Ofer Yizhar New serotype pAAV-CaMKIIa-ChRmine-oScarlet-KV 2.1-WPRE AAV8 Optogenetics Karl Deisseroth ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype ...

...Bachle  Listen to the Caspex podcast segment Steven Carr’s lab at the Broad Institute focuses on proteomics... to the C-BERST podcast segment Similarly to the Carr lab, Erik Sontheimer and his group from the University...

... MBP tag can be removed using Xa protease. The carboxyl-terminus of the fusion protein includes an AviTag...for affinity purification (see plasmid map and cartoon below). Including biotin in your culture and inducing...

...Bob Goldstein’s lab at the University of North Carolina has made CRISPR in C. elegans even easier - now... below.) After self-excision, the resulting loxP scar is located in this synthetic intron, and only the...