...target bases without breaking the DNA backbone, making them efficient and precise tools for altering DNA...the core ring structure intact, allowing them to make 4 out of the possible 12 base-to-base conversions...were engineering an ABE to reduce its tendency to make off-target edits to RNA. Like other ABEs, the editor...mammalian cells Researchers in the Xueli Zhang lab were making similar observations around the same time. (The...

...experiments. Best practices!). Making the find plasmids badge appear So how do you make this magical Addgene badge...favorite topic. If you have any other cool tricks to make PubMed browsing even easier and more useful, let...

...CVRHutchinson #SciArt #STEAM #WinterWonderlab @Addgene Make your own here!: https://t.co/AnltWfKuQg pic.twitter.com... wrapped gifts using life science catalog pages. Make your WFH space feel a little more festive this holiday... entering in (if you don’t include this, we will make our best guess). Follow @Addgene, use the hashtag...

...lid of the device. Select the desired method and make sure the parameters are correct. Transfer for 5–...

...recent blog post, we looked at how long it takes to make one plasmid and how much time Addgene has saved ...researchers. Assuming 5 hours of hands-on time to make each plasmid, we would have saved 5 million hours...will be moving into a bigger space so that we can make an even bigger impact - we are always on the lookout...

...topics you want to learn more about. Our post on “Making Your Own Competent Cells” has been extremely popular...additional questions. We’re happy to see scientists making their own cells to save money and create the tools...Replication By Kendall Morgan Save Time and Money by Making Your Own Competent Cells By Melina Fan Plasmids...

...Neuroscience meeting in Washington, D.C., we must be making a dent in the global coffee supply… My back-of-...we've spoken with are excited and passionate about making such tools open-access. Though it is time for me..., remember there are only 2 days left for you to make the most of this great opportunity – so if you’re...

...PFA-saponin, PFA-triton). The fix/perm solutions can make a significant difference in the efficacy of your... and the small difference in protein expression makes confirmation of antibody selectivity even more challenging...used has endogenous levels of expression, this can make the results more difficult to interpret, and a knockout...Antibodies. The Only Good Antibodies community works to make best practices in research antibody selection and...

...control of an otherwise ubiquitous promoter. You could make an entirely new mouse line with mCherry controlled...paired with any Cre-controlled gene of interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1...other viral vector tools in the years to come. We’ll make many more viral vector tools and resources available...

...is being an overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping forward...in a life sciences career but life has a way of making unexpected decisions. As someone who was not necessarily...additional person each day. It felt like I could actually make connections with people. These were some of the ...

...story. This year, we made significant strides in making science more accessible and reliable. We continue...experimental results. We also redesigned our website to make it easier to find what you need and to bring it ... every breakthrough enabled brings us closer to making an impact on humanity's greatest challenges. Thank...

...expression vector and attached a DNA barcode to each to make the library usable for pooled screens. Applications...different contexts.” It’s also possible you could make use of these plasmids in living animals, to explore... that’s thrilling to us,” Wood says. “We want to make these available as widely as possible.” The researchers...

...this interest in the ways science is shared and in making it easier to share, we take special note as the...Way to Share Science, in honor of a decade spent making plasmids more accessible and easier to share. As... As we prepare to ring in the New Year, let's make a toast to open science. And, please, share your favorite...

...capacity (8-10 kb) than other retroviral vectors. This makes it possible to use a polycistronic cassette that...Another challenge is that multiple transfection makes it difficult to control the dose of plasmid the ... pluripotency of mouse cells typically involves making chimeras by injecting iPSCs into blastocysts and... and Juan Carlos Izpisúa Belmonte. "Methods for making induced pluripotent stem cells: reprogramming à...

... the 1990s, biologists have spent a lot of time making things glow. Chances are you’ve used a GFP derivative...derivative to conduct subcellular localization studies or make a reporter construct. Fluorescent proteins (FPs)...the unexpectedly photoconvertible GFP proteins to make a new transgenic line could avoid problems in localization...

...partners and Tech Transfer Offices at universities to make COVID-19 research-related plasmids available more...Find plasmids available to industry scientists To make plasmid sharing more open for academic and industry... deposited plasmids at Addgene and would like to make them available to industry, please reach out at ...

... by making them brighter, more photostable, smaller, and less toxic to cells. They’d like to make near-infrared... muscles work inside a living animal. For those making use of these probes, he says, when in doubt, read...

...because I knew it would make me a better writer, but also because I knew it would make me a better scientist...mates, you add the individual figures and results to make the final product with an interesting introduction...

...best way to keep everyone on the same page is to make communication a non-negotiable part of your collaboration...Luckily, there are techniques and tools that helped make my experience run smoothly and can help other teams...meetings can be tricky, and it takes extra effort to make sure everyone in the meeting has an equal opportunity...

...toxicity or high off target effects, both of which make it a non-ideal drug. If a drug selectively kills...added QC is where CRISPR can save time and money to make sure quality candidates are selected.      ...Additional resources on the Addgene blog CRISPR 101: Making a Knock-In Cell Line Using CRISPR-Cas9 to Edit...