...addition to useful plasmids and toolkits available at Addgene.  C. elegans open resources The C. elegans Neuronal...source project that is creating the “first virtual organism in a computer.” This project has produced various...Kyoda et al., 2012).  C. elegans plasmid tools at Addgene In addition to the wealth of open resources described...highlight a few toolkits that we have available at Addgene that were discussed primarily at the workshops ...interest.  SapTrap Tool Kit for CRISPR Cas9 edits At Addgene, we have the entire SapTrap toolkit from the Jorgensen...resources about C. elegans and the plasmids we have at Addgene check out our worm expression resources page. This...PMCID: PMC4689222. Additional resources on the Addgene blog Learn more about SapTrap Read about CRISPR...

...maps. Resources on Addgene.org Sequence Analysis of your Addgene Plasmid Addgene Plasmid Guide     ...This post was updated on Dec 4, 2017. At Addgene, we continually use the Basic Local Alignment Search...databases, and elements of the BLAST search pages. At Addgene, we use blastn to identify any discrepancies in..., enter the common or scientific name into the Organism box. This small piece of information can significantly... viewing blastx results. When using blastx at Addgene, we use the default “Non-redundant protein sequences...NCBI BLAST website. Additional Resources on the Addgene Blog: Plasmids 101: How to Verify Your Plasmid...

...Guillain-Barré syndrome. In response, the World Health Organization has declared a global health emergency and called...corresponding portions of the Zika virus genome with Addgene to facilitate rapid sharing of these tools with... community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation...27160350. Find Plasmids from the publication at Addgene 2. Green, Alexander A., et al. "Toehold switches...e15272. PubMed PMID: 27090089. Resources on the Addgene Blog Read other Synthetic Biology Posts Listen...Your Next Experiment Deposit Your Plasmids with Addgene ...Used to Study Dangerous Viruses Resources on Addgene.org Check out All the Plasmids from the Collin's...

... at a professional organization to expand your skill set Resources on the Addgene website Become of ...your scientific conference? Resources on the Addgene blog Read other posts on scientific sharing, scientific...of a member of the Addgene depositor community ...

...field has its own framework: a particular way of organizing and connecting information and a logic to the...accessible to your audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

...types even when systemically administered in an organism. These features make AAV ideal for human therapies...2023). PubMed PMID: 36899921. Resources on the Addgene Blog New to Virus Work? Check out Our Primer for... Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik. Didem Goz Ayturk...Learn about Viral Vector Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All...

...you can accomplish this by employing a neat and organized format.  Columns. You can choose from either ...make it your own! Additional resources on the Addgene blog Check out this blog post on navigating the...

...Mice are a common model organism used to understand mammalian traits and genetically engineered mouse... a mouse strain. Additional resources on the Addgene blog Find our CRISPR blog posts Read blog posts... genome engineering blog posts Resources on Addgene.org Find more about Cre-lox Read our Genome Engineering...

...learning.” Addgene’s Director of Product Management Eric Perkins, who has been in many roles in Addgene since...work at Addgene. I posed this question to all nine of my interviewees. Fabienne Furt, Addgene’s Associate...resources on the Addgene blog Browse our science career blog posts Download Addgene's science career guide...stay in science.” Addgenies Beth Kenkel and Shannon Rinaldi in the Addgene lab! Read their Instagram... guide Resources on Addgene.org Learn more about working at Addgene ...Flynn and I am currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member...go back to school right away. Shannon Rinaldi, Addgene’s Lab Manager, was taking classes at her local community...

...frequently used species due to its use as a model organism in genetics and biochemistry. When using S. cerevisiae... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...

...promoters and/or fluorescent tags are available at Addgene. Shemesh OA, et. al. Nature Neuroscience. 2017...capturing information about spatial and temporal organization. The design use to detect PKA activity can be...sREACH. Find the FLIM-FRET acceptors here. See Addgene's FRET reference page for many other common FRET...  Various Addgenies...

...applications for funding from charities, commercial organizations, or universities may have someone senior from... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...

...Heckler. Additional Resources on the Addgene Blog Get Addgene's Take Aways from the FoR 2015 Symposium...Advocating for Science Symposium and Workshop, organized by Future of Research, Academics for the Future...

...PBS Additional Resources at Addgene Find the Ting Lab plasmids at Addgene Browse More Fluorescent Protein...function, and can have a specialized role within an organism. Mechanisms of epigenetic modifications The main...

...Marillonnet share with the scientific community via Addgene, the struggles of plant scientists and how they...Everybody's favorite parts for plant synthetic biology Addgene: Tell me about the Golden Gate Modular Cloning ...elements, which has not been available for plants. Addgene: Will the MoClo tools work in any plant? Patron... it a good laboratory workhorse. Tips & tricks Addgene: Do you have any particular tips for those looking...start and go ahead. I can see science speeding up. Addgene: Are there other tools you are working on for plants...interaction work and we’ve gotten some tools back from Addgene recently to explore their use in plants, including...technologies in the near future. Science on Twitter Addgene: You are active on twitter @nicolabiologist, where...

...including activating an oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate... K. 1993. PubMed PMID: 8513499. Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...University Medical Center are now available at Addgene along with a description of their experimental ...The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9, pAdSh.U6.gRNAS1, and pAdSh.U6...Maddalo et al., 2014). These tools are available at Addgene and could be useful to labs wishing to create this...including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9 (expresses S. pyogenes Cas9 from...technology guides, FAQs, and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT ....1089/hum.2015.087 Additional resources on the Addgene blog How to Design Your gRNA for CRISPR Genome...

...yielding promising results in the laboratory, and Addgene is accelerating this research by helping to make...proliferation. Hence it is imperative to the health of an organism that both classes of genes function normally to...possible. Connecting the research community - Addgene's cancer collection pages Scientists are making...latest resources? In response to this challenge, Addgene has launched an initiative to curate our collection...direct the investigator to a list of all available Addgene plasmids he or she can use to study it (as seen...section below. Michael G. Lemieux is a Scientist at Addgene with interests in education and scientific writing...Institute: Ras Pathway v2.0 Page Resources on the Addgene Blog Tackling Cancers' Drug Resistance with a ...

...From the beginning, one of Addgene’s goals was to ensure the scientific community obtained high-quality...the years to keep up with the latest tools. In Addgene’s early days, we would ‘spot-check’ critical regions...will describe the importance of bioinformatics at Addgene and how our search for solutions led us to a partnership...bioinformatics needs       Fig. 1: QC'ing data at Addgene is like standing under a Niagara Falls sized rainbow...than half of these sequences were generated by Addgene as part of our QC process. Automation and continuous...pipelining In many use-cases, including ours at Addgene, the overall purpose of a data pipeline is to perform... collection of scripts evolved into a single, organized pipeline and accelerated the processing of our...

...conferences Resources on the Addgene website Become of a member of the Addgene depositor community Learn...provide an on-topic answer by creating time to organize your thoughts. It’s likely you won’t know every... science communication.       Resources on the Addgene blog Read other posts on scientific communication...Learn about careers at Addgene ...