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We narrowed to 630 results for: proc

Showing: 321 - 340 of 630 results

  1. Lab to Office Culture Shock

    Type
    Blog Post
    ...the following email from a friend: “I'm in the process of onboarding a new employee who's coming directly...is good. You can experience a real onboarding process. Unless you were really lucky with your academic...

  3. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...central nervous system (CNS) orchestrates complex processes enabling organisms to control their movements ...tendency to brightly label somata but not neuronal processes. In contrast, smFPs can label fine neuronal structures...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...specific RNAs out of a pool of RNAs) or studying RNA processing in live cells. Indeed, groups have utilized dCas13...RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature 538(7624) (2016):270...

  5. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...You can see some of Joanne’s comments on the AMA process below, but we also wanted to share some thoughts...submission to avoid rousing the trolls. 3. The AMA process was simple and well organized. You can check out...

  6. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ...doses and timings of their drug treatments. The process of rising to such challenges involves using knowledge...up-to-date information about the industry, recruiting processes, and can connect you to a community of colleagues...

  7. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, ...Target Mouse Genes To begin the in vitro screening process, you should first develop primers to the approximately...

  9. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...communicated. The next step is to come up with a viable process that will avoid such miscommunications in the future...and examples that have guided your resolution processes in the past. Types of Resolution There are of...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...is displayed on the coats of bacteriophages, a process which links genotype and phenotype. Incubating ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...

  12. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...looking for, you are ready to begin the screening process. Want to set yourself up for a screening success...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...

  15. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...is upregulating HDR in mature tissues, as this process is necessary to precisely edit point mutations....

  16. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...value that helps you determine whether or not to proceed with a used or new item. To further illustrate ...we mentioned previously.  There is value in the process of creating a budget; you can share your budget...

  17. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms ...light-gated cation-selective membrane channel. Proceedings of the National Academy of Sciences, 100(24),...

  18. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...affect expression, before starting the selection process. Factors to think about are (1) your experimental...will allow you to efficiently move through this process.  Many other factors can also affect your AAV experiments...

  19. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

  20. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...template. HDR, however, is a very low-efficiency process, and usually involves the need for single cell ...successful edits. This is a very time consuming process and should not be undertaken lightly! Indeed, truly...
Showing: 321 - 340 of 630 results