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  1. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...a post that goes into the mouse genome editing process in a bit more detail in the coming weeks, but, ...Target Mouse Genes To begin the in vitro screening process, you should first develop primers to the approximately...

  3. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...for biological spectroscopy and microscopy." Proceedings of the National Academy of Sciences 114.23 (2017... real-time multicolor luminescence imaging." Proceedings of the National Academy of Sciences (2015): 201418468...

  6. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...communicated. The next step is to come up with a viable process that will avoid such miscommunications in the future...and examples that have guided your resolution processes in the past. Types of Resolution There are of...

  7. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...is displayed on the coats of bacteriophages, a process which links genotype and phenotype. Incubating ...single-chain Fv analogue produced in Escherichia coli. Proceedings of the National Academy of Sciences 85:5879–5883...

  8. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...looking for, you are ready to begin the screening process. Want to set yourself up for a screening success...then a single, bulk validation may be enough to proceed with your experimental plan. Validating knockout...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...is upregulating HDR in mature tissues, as this process is necessary to precisely edit point mutations....

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    ...value that helps you determine whether or not to proceed with a used or new item. To further illustrate ...we mentioned previously.  There is value in the process of creating a budget; you can share your budget...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    ...template. HDR, however, is a very low-efficiency process, and usually involves the need for single cell ...successful edits. This is a very time consuming process and should not be undertaken lightly! Indeed, truly...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms ...light-gated cation-selective membrane channel. Proceedings of the National Academy of Sciences, 100(24),...

  13. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...affect expression, before starting the selection process. Factors to think about are (1) your experimental...will allow you to efficiently move through this process.  Many other factors can also affect your AAV experiments...

  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ... appropriate secondary antibodies. The general process is similar to standard immunofluorescence, but ...spatial analysis of cells in complex tissues. Proceedings of the National Academy of Sciences, 117(52),...

  15. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...useful for the biotech industry, which is in the process of capitalizing on phage RBPs as diagnostic tools...DNA fragments with live host cells Now you can proceed with incubating this membrane with your cells of...

  16. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts: Part 1...have germline competent ESC lines. Second, the process is much quicker. It takes 3 months to generate ...

  17. Finding Your Perfect Job After University

    Type
    Blog Post
    ... controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was constantly...the technology is so advanced that most of the procedures in the lab are done with robots. Young scientists...

  18. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...your virus. Virus purification can be a tricky process. You have to centrifuge the culture supernatant...that favours virus precipitation. My tip for this process is to test several protocols that have been described...

  20. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    ...incorporation of that sequence into the zygote’s genome, a process that’s highly unpredictable. Transposons, however...injection into the cytoplasm of a fertilized egg, a process which is inefficient when DNA is injected. Sleeping...
Showing: 321 - 340 of 618 results