...ready for restriction digestion. Digest Your DNA Set up restriction digests for your PCR product and recipient...recipient plasmid to insert. It is also important to set up negative controls in parallel. For instance, a...

...selection mechanism. You’ll infect two sets of cells and subject one set to selection while the other serves...genome, while others are restricted to certain gene sets. In cDNA libraries, each plasmid contains a unique... be small, if they are designed to cover only a subset of genes, or very large (e.g. the Toronto KnockOut...

...profiles have limited their usability in clinical settings. On the other hand, vectors derived from AAV have...the gene RPE65. It is characterized by childhood onset of blindness and was thought to be untreatable until...

...case, you design your targeting vector such that a set of three LoxP sites flank the resistance gene and...targeted gene. Upon recombination, the GFP/NeoR cassette is inserted in place of exon 2. Thus, the targeted...sequence flanked by LoxP sites while the resistance cassette has been placed into an intron.    Figure...

...Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on whose website you can ...

...especially for systemic approaches, has its own unique set of obstacles. Thus far, several targeted therapies...

...sample, we’ll then load it on to our new MiSeq and set up the run making sure to note the appropriate barcode...

...happens when the antibody is produced from a new set of animals), and wrong application (different experiments...

...recommended pay scale – used by many institutions to set postdoc wages. The second event of note is a ruling...Assistant Professor of Sociology. University of Massachusetts Boston Christine Oslowski, PhD - Biotechnology...

...read-through. Chris Voigt’s lab has characterized a set of prokaryotic terminators and deposited several ...

...of several lasers simultaneously, can be used to set the laser output without manual manipulation of the...will open but will not be given an opportunity to “reset” and become receptive to light once again. Therefore...

...heterogeneous population of mutant cells, with each cell or set of cells containing a mutation in a different gene...

...supervisor, prioritize your action item list and set some approximate timelines. Be sure that you keep...

...and your initials. Labeling within a laboratory setting is important for organization, and it is recommended...

... you identify bacterial and yeast contaminants. Set up a routine to split cells Don’t be lazy about splitting...

...future in under 200 pages is an impressive task to set oneself. As a scientist who enjoys learning a little...

...have the idea that if you don’t get your PhD you’re set up for failure. Listening to Kenkel’s story has helped...

...convex scaffold and are randomized with a defined set of different trinucleotides (corresponding to different...

... notice for the first meeting so that people can set aside time in their schedules and read the paper ...). This notice can be shortened as the meeting settles in and people get the time recurrently carved out...

...chromoproteins are actually a subset of the fluorescent protein family. What sets them apart is the way they...