We narrowed to 954 results for: COMP

...information about the cell’s complexity. A cell with a high degree of internal complexity such as extensive membranes... prepare sets of controls to aid with compensation. Compensation is the process of correcting spectral...need to analyze a specific subset of cells in a complex population. Have you spent hours in the biosafety...cell’s size, granularity, and target protein composition. It is used for a variety of applications such...and detecting specific populations of cells in a complex tissue or sample (1). When combined with cell sorters...instrument collects information about the cell’s size, complexity and fluorescence intensity.   What flow cytometers... cytometers detect Cell size and complexity, measured using visible light scatter The optical system has...

...co-coregulator) partners. The coregulator ‘core’ complex is composed of a tightly-bound invariant group of proteins...coregulators exist as multiprotein complexes, a member of a single coactivator complex can serve as a rate-limiting...seeking to compliment these consortium-generated datasets with a systematic effort to compile NR- and coregulator-related...physiological processes are controlled by deliberate and compulsory actions instigated by the central nervous system...factors found in metazoans. The superfamily is comprised of 48 human and 49 murine members. NRs permit ...metabolic, and immune response programs. NRs also encompass one of the most successful targets for drugs currently...interact directly with NRs) exist in steady-state complexes with secondary coregulator (or co-coregulator)...

...iPSC cell lines, which can complicate comparisons made between lines. Incomplete silencing of transgenes ...are prone to incomplete silencing of reprogramming transgenes, which leads to incomplete reprogramming... heterogeneity between clones. This can complicate comparisons made between samples, i.e. transgene expression...efficiency of reprogramming when compared to other methods, even when compared to integrating viral approaches...less time to complete. Morphology is a simple and fast to evaluate. iPSCs should form compact colonies that...and have a higher efficiency of generating iPSCs compared to other delivery methods. Retrovirus has a cloning...lentivirus has similar rates of reprogramming when compared to MMLV-derived retroviruses, it is not as well...

.... The team at the Victoria Makerspace is composed of computer scientists, engineers, and biologists working... elegans as a computer model. When I first heard about it, I got excited from a computer scientist perspective...defects above 25 degrees. Computer scientists and biologists work together to compare the predictions about...interests in biology and computing. In 2009, he founded a team at UVic to compete in iGem, an MIT-sponsored... really epitomizes what the makerspace is. The computer scientists can get involved in modelling, the ...Developing these skills relies upon biologists and computer scientists learning and communicating with one...coding the molecular biology of the worm into a computer model that recapitulates how molecular biology...

...of oxidation (dehydrogenation). MitoTimers are composed of a Timer protein fused to the mitochondrial ...upregulate an alternative pathway or induce other compensatory mechanisms. How can protein function be dissected...’s lab, acutely depleted proteins from their compartment of action via sequestration. The strategy relies...outer mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of ...May 1;126(Pt 9):2102-13. Assemble multiple component plasmids from building blocks via Golden GATEway...GATEway Cloning Frustrated by the complexity of assembling recombination of transgenesis constructs? A new...cloning kit simplifies the cloning process for complex DNA constructs, particular for those involving ...

...different compositions of the Cascade complex. Created with BioRender.com.   The Cascade complex Cascade... an important component of the CRISPR Cas3 system. Most Cascade complexes are comprised of a combination...systems work using a multi-Cas complex called Cascade (CRISPR-associated complex for antiviral defense). Cascade... Cascade complex from CRISPR type I-E. Created with BioRender.com. Thanks to this complex, developing... It can be difficult to ensure comparable expression of all components of Cascade for maximum efficiency...Cascade-Cas3 plasmids in Addgene's collection! A "complex" system Cas3 is part of the Class 1 CRISPR family...from different species and will have different compositions of Cascade. Although seemingly ubiquitous in...

... pre-mRNA splicing in a compound screen. Their reporter system is comprised of two plasmids: Luc (intronless... Vacuolar Compartment mCherry-Rab5 BFP-Rab5 GFP-Rab5B Vacuolar & Budding Compartment GFP-Rab4B ...insights into the make-up of multiprotein complexes like the EPC1 complex.. Going one step further, they also...about it! Split Cas9 system The Cas9 protein is composed of an n-terminal DNA recognition domain and a ...sequencing or other readout. Cellular memory is accomplished by the use of sequence-specific enzymes (e.g...calculated (the BRET ratio), which allows for compensation of varying fusion protein expression levels....more powerful LID, the authors recently used computational protein design, phage display and high throughput...

...important that the digest go until completion. The time required for complete digestion varies for different...want to use electro-competent cells instead of the more common chemically-competent cells. You should perform...fragment of DNA (insert) that has been cut by compatible restriction enzymes. An enzyme, DNA ligase, then...plasmid to the new fragment thereby generating a complete, circular plasmid that can be easily maintained... are cut with restriction enzymes to generate compatible overhangs that allow them to bind. Ligase is ...your backbone and insert - these both must have compatible cut sites for restriction enzymes that allow ...    Alternatively, this whole process can be completed using a single enzyme if your insert is flanked...

... work for biotechnology companies and help other researchers use the company’s products successfully. ...guidance, and resolve complaints about products. As you might expect, different companies produce different... duties. Bigger companies have dedicated technical support teams, sometimes comprised of several layers...cells (manufactured by one of the larger biotech companies), and as far as I remember, it was the first time...time that I was absolutely convinced it was the company’s fault. I contacted them to express my concerns...rather painful to get there: I had to send the company detailed documentation of my results, answer loads...takes some time (several years) to build up comprehensive lab experience. With this valuable training ...

...lead to comparable experimental results. Using PiggyBac transposons, the Woltjen Lab compared different...robust engineering. These components are provided in Golden Gate compatible backbones that can be combined...latest hot plasmid updates!     Tol2 Gateway-compatiable toolbox for studying the nervous system The... Cole lab recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists to quickly... neuorodgenerative diseases. The Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated...vectors that have standard cloning sites or gateway compatible cloning sites for easy cloning of your genes ...adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst the zebrafish...