...of His-tagged human kinases in bactera. Listen to Our Segment on The Human Kinase Domain Constructs Kit...Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's Lab -...

...taking steps to increase plasmid distribution and disseminate knowledge around the globe. Addgene’s progress...inception in 2004. In the near future, we hope to eliminate much of white space on this map and increase plasmid...the Addgene Blog Learn About Our Recent Visit to China Read Zafira Castano Corsino’s Recent Post on the...

...project that would be a big deal if it worked out.”- Melina Fan, Chief Scientific Officer “Choose your PhD ... Tsang, Science Communications and Marketing Coordinator Networking “Many, perhaps most of the most interesting...customized letter to the place that you are applying.” - Melina Fan, Chief Scientific Officer If there’s a career...

...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... work with in neurons or you might use it in combination with Flp-FRT. Additionally, you could use the... cre-recombinase...

...lab heads have revealed that a combination of hard work, determination, passion, and patience are required... time for fun and life outside of the lab? This final post in the PI Interview Series investigates how...

...distributor partners, with the launch of antibodies in China and expansion of viral services available in South...Innovation (IPI), in which Addgene will distribute recombinant antibodies (rAbs) developed at IPI. The first...partially through work with our distributors in China, Korea, Japan, India, and Brazil. Through an expansion...

...this goal could involve participating in interdisciplinary seminars, such as those in bioinformatics or chemistry...lies within a biological lab. Engaging in such seminars exposes you to discussions beyond your immediate...

...’s functions, besides structure and isotype determination, don’t generally affect antibody applications...stabbing” and “poisoning,” but let’s be honest, the elimination style the immune system is most famous for is...domain as only providing structure and isotype determination, remember that it can do so much more than that...

...physical association of combinations of different Gα, Gβ, and Gγ subunits. When inactive, these subunits are...addition of the endogenous ligand or drug. In the inactive state, the luminescent chimeric protein Gα-Rluc8...

...perfectionists, it took us a full two months to finally decide the LEGO laboratory was good enough. There...box, the Big Bang Theory set, and then use our imaginations to mold it all into a full laboratory. It is...It was a great treat for us to put our rusty imaginations to use in this world where we are often trained...

...as examples for this post. The D10A mutation inactivates the RuvC domain, so this nickase cleaves only...and images in this post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome...For more information and a link to the archived webinar, click here. We would like to thank IDT scientists...

...significant improvement and simplification of the original technique, specifically in terms of initial steps...excellent additional measure of DNA binding sites in combination with existing ChIP-seq data sets, of which there... a graduate student at the University of North Carolina in Chapel Hill, where he studies enhancers and...

...then in my final year of graduate school, I actually served as the student course coordinator. With the...’d put in outside the lab had paid off. “The combination of the editing and teaching experience that I...

... organs located deep in the body, like the gastrointestinal tract. Require Administration of Substrate...coli aided in ultrasound imaging of the mouse gastrointestinal tract. The E. coli strain Nissle 197 (ECN)...probiotic strain that can colonize the mammalian intestinal tract and has been used for 100 years to clinically...

...the advantages of viruses and naked DNA while eliminating some of the drawbacks. Specifically, viruses ... are many such sites in the human genome). The original plasmid is empty following the removal of the ...transposon sequences and then testing various combinations of changes. The basic functional version of ...

...North Carolina Research Triangle area, scientists give talks and science cafés at the North Carolina Museum... surprised to find that his friends and family finally understood why he cared to study mice on some distant...

... precious in less resourced areas of the world.  Nina Dudnik, Founder of Seeding Labs, tells the story...Outreach Scientist, Andy Baltus. Addgene founder, Melina Fan, recently shared our protocol for making competent...this was hilarious.  Birth of the baby gel box Finally, taking conservation to new levels, I was bothered...

...forHomologous Recombination (HR)? How long should the homology arms be for efficient recombination? A3: The... the CRISPR/Cas system with your homologous recombination (HR) template, you could then: Confirm your...

...kill bacteria, or inhibit their growth. Although originally sourced from natural products, many common antibiotics... are trying to disrupt or delete, which both inactivates the gene and acts as a marker for the mutation...cassettes and your gene of interest is cloned into/inactivates or completely removes (in the case of Gateway...

...Di Ventura (@barbara_synbio) May 13, 2021 The original AraC transcriptional regulator Their system is...does BLADE have over AraC? When compared to the original AraC system, BLADE is simply more precise. “We...light switch and shuts off just as quickly. The original AraC system, which uses arabinose, requires many...

...unsuccessful. Nothing more joyful that getting to the final step and the transformed bacteria refuse to grow...(Jackson ImmunoResearch, 016-050-084; 0.2 μg/ml final). Both rabbit (EMD, AB3596) and mouse (NEB, E8032S...matrices, not so much. Maybe a calcium-independent proteinase site could be used for plasmid version 2.0? Instruments...

...Washington University in St. Louis provides a combinatorial indexing strategy that simultaneously tracks...cell type that can differentiate into hepatic or intestinal cell types. MEFs were reprogrammed into iEPs ...2019). Key takeaways of CellTag CellTag is a combinatory cell-indexing tool that combines scRNA-seq and...

...mobilized to figure out how to deliver when labs finally opened.  I was awed by the teamwork and initiative...One function that rose to the challenge was our Finance Team. With Addgene closed for almost two weeks ...for unknown amounts of time, we were facing a financial crisis. As a nonprofit organization, Addgene charges...

...outside of academia, a job as a ‘Cancer Marketing Coordinator’ came up at Abcam. Unlike other job descriptions...scientists think that they are unable to tick these final two boxes, but I knew there would be a way around...marketing gives you a great introduction to the financial world. It teaches you about selling products which...

...This is the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series...SMART.” SMART goals are Specific, Measurable, Attainable, Relevant, and Time-bound. It is a good idea ... open communication Feel valued by giving back Final tips for success Make mentoring meetings a priority...

...Generated against full length GFP and converted to recombinant form by the NeuroMab Facility Isotype IgG2a ...a polyclonal, with the reproducibility of a (recombinant) monoclonal. Another reason is that some antibodies...  et. al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex ...

... credit for small contributions. We strive to eliminate these two barriers, creating a community where...other scientists and to help in Crowdfight’s coordination work.  We also encourage you to file a request...Biomédica de Salamanca (IBSAL) and one of the coordinators at Crowdfight. She is currently based in Spain...

...assemble hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences...heterogeneous and/or mixed clones present in the original sample. At seqWell, we have worked with Addgene...prepared as plexWell™ libraries, sequenced on an Illumina® sequencer, and then analyzed with our plasmid...

...Cas9 activators available in lentiviral format. Finally, while we can’t currently produce custom gRNA-containing...representation in the viral prep, infect target cells, and, finally, conduct the screen. To help alleviate some of...any questions or comments to [email protected]. Finally we’d like to thank all those who are allowing us...

...these materials may not be available from their original sources. Figure 1: Biological repositories...availability of this strain from The Jackson Laboratory. Originally developed and characterized by Stanley Perlman...materials to be deposited into repositories, and financial support to maintain repositories’ capacity for...