We narrowed to 794 results for: Tec;

...The sky is really the limit for this emerging technology! Resources and References References Orguc, ...modulation of gut and brain neural circuits. Nature Biotechnology, 1–13. https://doi.org/10.1038/s41587-023-01833...

...secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect Protein B...

...STAR (Situation, Task, Action, Result) interview technique, which has been addressed in much better pieces...story should be too. Use drama Use any rhetorical technique you have in your arsenal to make sure people pay...

... to detect mutants, but this method limits screening to a few hundred worms, making the detection of rare...

...especially for life science researchers. Many biotechnology companies are looking for PhD or Master’s level...capabilities, courses and office skills. I removed my techniques and protocols, courses taken during my PhD and...

...translational approaches for scientific tool and technique development. However, careers outside of the lab...Management Leadership Networking University technology transfer office positions   Market analyses...

... to learn more about this killer illumination technique! What is a photosensitizer? First, for a few ...genetically encoded photosensitizer. Nature Biotechnology,24(1), 95-99. doi:10.1038/nbt1175. PubMed PMID...

... where the mutation can be placed. With other techniques, when mutations are spaced farther apart, it ...understanding the minimal genome. Many of these techniques require more complex DNA assemblies and improvements...

...plasmids: Genetics & Heredity, Cell Biology, Biotechnology & Applied Microbiology, Biochemistry & Molecular...research focuses on a range of topics including biotech and research tools, computing performance, and ...

...developers’ willingness to make their CRISPR technologies available to the academic research community...Addgene, we’ve helped distribute many of these technologies in plasmid form and are proud to have facilitated...

...particularly interested in advanced in vitro models technology. Follow him on twitter @FRE_SCI.   Additional...resources Find protocol videos to teach basic lab techniques Plasmid Land: The plasmid cloning game ...

...viral particles per cell, you may not be able to detect AAV-mediated gene expression in a target cell. ...confirm. Luke Makana Hanley is a Research Team Lab Tech at Addgene working on methods to improve AAV production...

...materials available rapidly to scientists (Nature Biotechnology 2020). The continued success of repositories...j.tim.2006.05.007 (2020) Open for outbreaks. Nat Biotechnol 38:377–377. https://doi.org/10.1038/s41587-020...

...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...you stay connected. If possible, test out the technology before the conference begins.“There were some...

... eat, and rest. Try the Pomodoro Technique or the Flowtime Technique. Co-work virtually. With co-working...

...responsiveness, they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and...iGluSnFR3 variants should enable scientists to robustly detect signaling events in the brain and beyond. These...

...limitations. First, FRET signals are often difficult to detect, and thus quantify, due to small fluorescence changes...induces apoptosis by activating caspase-3, and detected fluorescence within 2 to 5 hours, a time consistent...

...transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9... for microscopy by: Susanna Stroik Microscopy techniques have been moving at the speed of light over the...

...measurements such as patch clamp recordings. However, technical difficulties such as small fluorescence changes...QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect action potentials in worms with fluorescence changes...

...contrast to recent genome engineering technologies, stem cell techniques are neither fast nor inexpensive....

...Culture by Gibco Whenever I start to learn a new technique, I try to look for a resource to help me. I’m .... When I started learning proper cell culture technique, I tried to find an app that could help me with...

...thought that they function as a kind of armor to protect their host from predation (Petroni et al 2000)....the Addgene Blog Read About Other Hot Plasmid Technologies Read Other Synthetic Biology Posts  Suggest ...

...fluorescent proteins whose spectra overlap, there are techniques, like spectral unmixing, which can be used to...green and red proteins are generally the most detectable and so should be used to tag your least abundant...

...introduction of DNA into bacterial cells); however, the techniques and reagents vary. Plasmid transfection into ...positive selection; however, negative selection techniques can be used in conjunction with positive selection...

...With increasingly sophisticated visualization techniques it is possible to follow the interactions between...microvillar search and stabilization during ligand detection by T cells." Science 356.6338 (2017): eaal3118...

...patient’s tumor sample, use cutting edge genomic technologies to map the mutations that are present, and use..., these early pilot studies have demonstrated technical feasibility and proof-of-concept. If such approaches...

...Without these scientists and the support of their technology transfer offices, Addgene would not be able to...educational materials, and provides viral vector technical service (details about virus production protocols...

...use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency of an experiment...editing, regulating and targeting genomes. Nature Biotechnology 32:347–355 . https://doi.org/10.1038/nbt.2842...

...Tim Miles, PhD, Director of the CLOVER Center at CalTech? If so, you may have submitted a question that ...with no more ado, the questions!  AAV Prep and Technique Q1: Is there any way to deliver a long gene (>...

...infrared fluorescent protein tag which let the team detect its co-localization with a membrane-localized mCherry...about using the secondary nanobody toolbox for immunodetection Resources on Addgene.org Find nanobody expression...