We narrowed to 954 results for: COMP

...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...

...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...

...neutralization or targeted destruction.  Antibodies are composed of two light chains and two heavy chains. The ...the use of less toxic adjuvants; 2) generating complex antibody chips that allow assessment of many protein...

...lab equipment on? This applies to water baths, computers, and more. Consolidate your autoclave runs. In...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...

...measure will ever adequately capture a notion as complex as “scientific impact.” As we look ahead to the...Science - The Book Bollen J, et al. "A Principal Component Analysis of 39 Scientific Impact Measures" (2009...

...’s role in infection also makes it a critical component of vaccines and the target of neutralizing antibodies...Science Translational Medicine have made their complete collection of relevant papers freely available...

...optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells. The Feng Zhang...efficiency can be controlled by manipulating complementarity between the gRNA and target. Read the paper...

...about careers that we’ve compiled our top career blog posts into one comprehensive, downloadable guide: the...

... more primitive version of the larger and more complex Cas9 and Cas12 proteins. Cas14 cleaves ssDNA But...the detection of RNA, dsDNA, and now ssDNA. By comparing the ability of Cas12 and Cas14 to detect SNPs ...

...finishing your PhD or perhaps you have almost completed a postdoctoral position… or two. You have learned...technique and be able to talk and own these accomplishments. Employers and academic departments want to...

...which decrease the rate of off-target genome edits compared to rat APOBEC1. Now, they’ve engineered SECURE... increased editing efficiency of a target site compared to a mixture of two base editor enzymes in mammalian...

...gRNAs to knockout genes – are essential screening components, the model system and the assay are just as critical...him that he has the best words and fingers of a completely normal size.      References: 1. Doench, John...

...Medical Center. “While we do not yet have a comprehensive understanding of how cancers become resistant...they identified a set of one to three mutant complementary DNAs (cDNAs) representing core nodes that, when...

...having no significant effect on cell viability compared to a wild-type control. Approximately ~22% of ...scientists understand how a given gene is involved in a complex pathway. Some of the most heavily-studied genes...

...plasmids are available either individually or as a complete kit from Addgene. References 1. Goulas, Theodoros...Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications...

... online calendar and can be accessed from your computer or smartphone. Evernote allows you to create notes...ELNs) have gained traction with pharmaceutical companies, academic labs have been slower to adapt, likely...

...Award, the Rare Genomics Institute partners with companies to provide technological resources for those studying...peritoneal mesothelioma. Dr. Nabavi and colleagues have compiled data from primary peritoneal mesothelioma tumor...

...academic and industry scientists. This partnership complemented each other's strengths: Ginkgo Bioworks’ infrastructure...distribute such a large deposit. Once the project is complete, Ginkgo will have synthesized approximately 3,...

... investigators the agility to rapidly initiate complex research projects that they would otherwise not... Here’s what caught our eye recently (not a comprehensive list). #coronavirus #SARSCoV2 #Covid19researchA...

...works and in what doesn’t, and feedback are vital components of building both the community and the database...deep phenotyping and spatial analysis of cells in complex tissues. Proceedings of the National Academy of...

...neuroscience is becoming more and more versatile. To complement the wealth of genetically encoded tools available...only reagents, but also method information. To complement these efforts, we’re beginning to share data ...

...with the previously established physical map. In comparing pairs of targets ~2 Mbp apart, they noticed that...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

... next steps and follow up especially in large companies. It is fine to address the cover letter “To Whom...If the organization is small, any person at the company might be able to help you. If the organization ...

...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

..., yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald...color variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the ...

.... “Initial and sustained motivation is a huge component of success.” 2. “Enjoy Teamwork.” Be friendly...friendly, open, sharing, rather than intensely competitive. Be curious and interdisciplinary.   3. “There are...

...lentiviral CRISPR library systems described in companion papers in Science in December were developed as...colleagues, including David Sabatini and Eric Lander, comprise 73,171 guide RNAs (gRNAs) covering a total of ...

...], so making it a success feels like a real accomplishment.” He started working on them shortly after ...Development Manager at eScience Labs, an educational company that creates hands-on science lab kits and digital...

...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...Yes Longevity in Serum?* No Yes Table 1. Comparing SpCas9 and GeoCas9. *For in vitro Cas9 RNP activity...

...first collapse arg2 and then arg1 vesicles. By comparing ultrasound images pre-collapse and post-collapse...mouse colon, imaged via ultrasound, and the images compared to mice injected with ECN expressing lux. arg1...

...an ‘-omics’ study or is resistant to specific compounds, links to publication references, and cross-references... publications cited. If the information seems incomplete or unclear, Bairoch follow up via email to the...

...started running on a treadmill. After being with the company for about six months, I learned there was a Genzyme-based...visit us and watch fellow Run4Rare team members complete the Boston Marathon, he wanted to run and play...

...or leading. All three roles are important to accomplishing any goal or finishing any project. Doers are...Leadership Insights for Engineers, Scientists and Computer Professionals as Leaders, by Ken Graham, Ph.D....

...training from an organization such as COMPASS. Although COMPASS has a special interest in ocean science...

...typical research project can take several years to complete and has multiple benchmarks. Think about what ...Researchers should  pick a topic that “looks at the complexity of biology and break apart a piece that can address...

...protospacer adjacent motif (PAM). Once the CRISPR/Cas complex binds to the foreign DNA, a cut is made to destroy...eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have been characterized...

...individual diversity to get from gamete to a visibly complex - and unique - adult. My initial research directions...evolutionary perspective brought by studies of comparative anatomy showed me that some of the most fascinating...

...photobleaching, and achieve better signal in vivo as compared to imaging with broccoli aptamers or MS2 tags ...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...

...here and now. Our closure was earlier than most companies. Since the start of this crisis, we’ve been very... in our building that someone from a different company had tested positive for COVID-19 (it turned out...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ...secondary structure and high GC content, but some companies now offer Sanger sequencing conditions which can...