We narrowed to 801 results for: MAK

1. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   ... DNA repair factors (which repair those breaks) makes Cas9 extremely effective as a genome editing reagent...

2. Advice for Scientists Starting a Lab

   Type

   Blog Post

   ...resources for Early Career Scientists, including their "Making the Right Moves: A Practical Guide to Scientifıc...

3. Viral Vectors 101: The AAV Retrograde Serotype

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   Blog Post

   ...efficiently deliver genetic information or tools, making them useful tracers, but finding a virus that works...

4. Plasmids 101: Screens vs. Selections

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   Blog Post

   ...worse yet have a screen that doesn’t yield any hits. Make sure you plan ahead and consider these questions...

5. Luminescent Imaging with Nano-lanterns

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   Blog Post

   ...to the low intensity of luminescent proteins. To make luminescent imaging a reality, Addgene depositor...

6. Top Requested Lentivirus and AAV of 2016

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   Blog Post

   ...plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging...

7. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   Blog Post

   ...Archived.” This status allows us to store plasmids and make them available to scientists without performing ...

8. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   ...elicit a strong immune response, factors that may make it especially suitable for in vivo research and ...

9. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

10. Early Career Researcher Toolbox: Social Media for Scientists

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    Blog Post

    ... social media as a scientist? pic.twitter.com/rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December...

11. Antibodies 101: Isotypes

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    Blog Post

    ...antigen of interest. Isotype controls are used to make sure the staining you see is due to target-specific...

12. AAV Q&A with Tim Miles

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    Blog Post

    ...one vector.   Q2: Do you think it is possible to make an efficient AAV prep for a 4.2 kB insert with the...

13. Hot Plasmids - February 2022

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    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

14. Plasmids 101: Methylation and Restriction Enzymes

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    Blog Post

    ...or for diagnostics, we suggest double checking to make sure your results will not be affected by methylation...

15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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    Blog Post

    ...machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang...

16. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

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    Blog Post

    ... and devoted 100% of his energy and effort into making the startup self-sustainable. Within a year from...

17. Giving gRNAs a Facelift - Synthetic and Beyond

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    Blog Post

    ...modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget is to simply...

18. Choosing the B(right)est Fluorescent Protein: Photostability

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    Blog Post

    ...issues with some of the experimental designs used to make these measurements and hope this information can...

19. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

21. Transferable Skills Guide: Cross-team Communication

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    Blog Post

    ...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...

22. Transferable Skills Guide: Problem Solving

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    Blog Post

    ...other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these...

23. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ... away. As a result, variable gene expression can make data hard to interpret or can interfere with the...

24. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ... the main source of IL-36 cytokines in the skin making it important to study what is happening within ...

25. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

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    Blog Post

    ...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

26. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    ...Be mindful of the restriction enzymes you chose. Make sure that enzymes that you are using are not blocked...

27. Human Germline Editing Using CRISPR

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    Blog Post

    ...differences in editing between iPS cells and embryos make a case for conducting research in embryos should...

28. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

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    Blog Post

    ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

29. New Tool for Lineage Tracing: The ClonTracer Library

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    Blog Post

    ...deposited the library with Addgene because she wanted to make it as easy as possible for researchers to use this...

30. Plasmids 101: Cre-lox

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    Blog Post

    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

31. Working with Nuclear Receptors

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    Blog Post

    ...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

32. Viral Vectors 101: Virus Safety

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    Blog Post

    ...having an ‘off’ day – you are both more likely to make a mistake and more likely to want to cut a few corners...

33. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ...antibodies rather than generally reactive ones. And make sure to check for any reported cross-reactivity ...

34. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

35. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    Blog Post

    ...-Cas system. Conclusion CRISPR is a great way to make those genome edits. But, just as important is a ...

36. Synthetic promoter AAVs for cell-type specific expression in retinal cells

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    Blog Post

    ...tracing experiments where lower levels of labeling makes it easier to trace a neuron’s projections. Generating...

37. Early Career Researcher Toolbox: Finding Relevant Papers

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    Blog Post

    ...Career Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...

38. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

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    Blog Post

    ...stopping the HRP-TMB reaction more quickly. Just make sure to incorporate the dilution factor in your ...

39. Hot Plasmids and Viral Preps - January 2021

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    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

40. Adenoviral Vector Production and Troubleshooting

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    Blog Post

    ...Interestingly, the strong immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis...