We narrowed to 795 results for: Tec;

1. Improving Transduction Efficiency with Exosome AAVs

   Type

   Blog Post

   ...association of AAV particles with membrane vesicles protects them from antibody recognition and inactivation...

2. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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   Blog Post

   ...Bourges for editing this post and sharing her technical expertise in persistent identifiers. Additional...

3. What Good Citizenship Can Do for Reproducibility in Science

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   Blog Post

   ...are often very subtle, and it can take a lot of detective work to figure them out. But, when they come to...

4. New and Upcoming Viral Vectors - September 2019

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   Blog Post

   ...of calcium dynamics. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...

5. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

   ... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging...

6. Summer SciComm Series: A PhD in Science Communication

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   Blog Post

   ... remains inaccessible, behind paywalls and in technical jargon that even scientists in neighbouring disciplines...

7. Predicting Adverse Reactions to Monoclonal Antibody Drugs

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   Blog Post

   ...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...

8. Creating accessible biology activities in schools with BioBits

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   Blog Post

   ...Browse our protocol videos to learn simple lab techniques Check out our molecular biology reference pages...

9. Fluorescent Proteins 101: Luciferases

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   Blog Post

   ...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins...

10. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

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    Blog Post

    ...Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid Backbone ...

11. Antibodies 101: Flow Cytometry Controls

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    Blog Post

    ... immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School...

12. Viral Vectors 101: Viral Vector Elements

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    Blog Post

    ...immune response to the virus to allow it to evade detection by the host. Of note, recombinant adenovirus is...

13. Viral Vectors 101: Biosensors

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    Blog Post

    ...as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from ...

14. Viral Vectors 101: Viral Applications

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    Blog Post

    ...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...

15. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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    Blog Post

    ...plasmids available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...

16. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    Blog Post

    ...Cas domain compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional...

17. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    ... of Murine Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832  Lipman ...

18. Targeted Mutagenesis with EvolvR

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    Blog Post

    ...EvolvR-ing to targeted mutagenesis." Nature biotechnology 36.9 (2018): 819. Additional resources on the...

19. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post

    ... not contain the trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided...

20. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    Blog Post

    ... can be especially important for base editing techniques, where Cas9 must be directed to a very specific...

21. Plasmids 101: Screens vs. Selections

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    Blog Post

    ...added baseline component. Alternative screening techniques While CRISPR and drugs dominate the screening...

22. Hot Plasmids - May 2022

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    Blog Post

    ...Kaufman, et al, 2022.    This new barcoding technology would allow researchers to avoid the off-target...

23. 7 Tips to Secure a STEAM Internship This Summer

    Type

    Blog Post

    ...Several days prior to the interview, inspect your technology, background, and lighting for a smooth performance...

24. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    ..., small deletions and point mutations will go undetected by restriction digest methods.    Resources...

25. How to Keep a Lab Notebook for Bioinformatic Analyses

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    Blog Post

    ... in the same directory where it was run. This technique leaves a trail that further helps you keep track...

26. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    Blog Post

    ...a gBlocks® Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9...

27. Plasmids 101: Yeast Vectors

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    Blog Post

    ...proteins yet they still share many of the same technical advantages that come with working with prokaryotes...

28. Human Germline Editing Using CRISPR

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    Blog Post

    ...achieved any end goal not available with current techniques like PGD.  Reference Ma, Hong, et al. "Correction...

29. Viral Vectors 101: An Introduction to AAV

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    Blog Post

    ...Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al....

30. All in a Twist: dsRNA

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    Blog Post

    ... RNA-induced silencing complexes (RISCs), which detect dsRNA in the cytoplasm and use it as a guide to...

31. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

    ...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate those...

32. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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    Blog Post

    ...first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations and a handout...

33. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

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    Blog Post

    ...strong in this one. After all, the system built to protect us now seems to attack us by releasing mitogenic...

34. Seven Tips for Using LinkedIn as a Scientist

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    Blog Post

    ...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...

35. Antibodies 101: Affinity Reagents

    Type

    Blog Post

    ...sensitive drug delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: ...

36. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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    Blog Post

    ...methods such as patch-clamp electrophysiology, a technique that’s used to measure electric current of ion...

37. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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    Blog Post

    ...expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. ...

38. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    Blog Post

    ...The first time through, just get an idea of the techniques used in the research, but wait to sort out any...

39. Career Coaching for Scientists: Why and Where Do I Find One?

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    Blog Post

    ...is a proven coach who helps people in science and tech get jobs. She is also a very popular speaker and...

40. A Look at Addgene's QC Process

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    Blog Post

    ...dedicated scientists with expertise in sequencing technologies and bioinformatics. Through their efforts, the...