Addgene

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New and Upcoming Viral Vectors - December 2019

Type

Blog Post

...ubiquitous expression. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...
Hot Plasmids: FRET-Based Biosensors

Type

Blog Post

...not known.” Transition Metals Maarten Merkx of Technische Universiteit Eindhoven has similar interest in...
Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

Type

Blog Post

...philosophical implications. As an example, a group at Caltech used an optical fiber together with optogenetic...
Improving Transduction Efficiency with Exosome AAVs

Type

Blog Post

...association of AAV particles with membrane vesicles protects them from antibody recognition and inactivation...
Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

Type

Blog Post

...Bourges for editing this post and sharing her technical expertise in persistent identifiers. Additional...
Creating accessible biology activities in schools with BioBits

Type

Blog Post

...Browse our protocol videos to learn simple lab techniques Check out our molecular biology reference pages...
FPbase: A new community-editable fluorescent protein database

Type

Blog Post

... on the Addgene Blog Fluorescence Microscopy Techniques - Which is best for me? A Practical Approach ...
New and Upcoming Viral Vectors - September 2019

Type

Blog Post

...of calcium dynamics. (For more details on this technology, see our blog post Overview of AAV Encoded Calcium...
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

Type

Blog Post

... tagged proteins, a common problem of the AID technology (Yesbolatova et al., 2019). Find the mAID tagging...
Predicting Adverse Reactions to Monoclonal Antibody Drugs

Type

Blog Post

...of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, 128–142. https://doi.org/10.1016...
Summer SciComm Series: A PhD in Science Communication

Type

Blog Post

... remains inaccessible, behind paywalls and in technical jargon that even scientists in neighbouring disciplines...
Viral Vectors 101: Inverted Terminal Repeats

Type

Blog Post

..., small deletions and point mutations will go undetected by restriction digest methods. Resources...
Scientists Map the SARS-CoV-2-Human Interaction Network

Type

Blog Post

...constructs are tagged with a 2xStrep-tag for easy detection and affinity purification. All but one are in ...
What Good Citizenship Can Do for Reproducibility in Science

Type

Blog Post

...are often very subtle, and it can take a lot of detective work to figure them out. But, when they come to...
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Type

Blog Post

...Assembly Blog Post Choosing a Molecular Cloning Technique Choosing Your Perfect Plasmid Backbone ...
Antibodies 101: Flow Cytometry Controls

Type

Blog Post

... immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School...
Targeted Mutagenesis with EvolvR

Type

Blog Post

...EvolvR-ing to targeted mutagenesis." Nature biotechnology 36.9 (2018): 819. Additional resources on the...
15 Years of Addgene: The Top 15 Plasmids

Type

Blog Post

... not contain the trademarked Tet-On(R) sold by Clontech. For additional information, see the manual provided...
How to Keep a Lab Notebook for Bioinformatic Analyses

Type

Blog Post

... in the same directory where it was run. This technique leaves a trail that further helps you keep track...
Hot Plasmids - May 2022

Type

Blog Post

...Kaufman, et al, 2022. This new barcoding technology would allow researchers to avoid the off-target...
Viral Vectors 101: Viral Vector Elements

Type

Blog Post

...immune response to the virus to allow it to evade detection by the host. Of note, recombinant adenovirus is...
Viral Vectors 101: Biosensors

Type

Blog Post

...as FRET-based biosensors have been engineered to detect a wide array of neurotransmitters ranging from ...
Viral Vectors 101: Viral Applications

Type

Blog Post

...over a hundred years ago. Since then, medical technology has changed a lot, but viruses have stuck around...
Plasmids 101: Screens vs. Selections

Type

Blog Post

...added baseline component. Alternative screening techniques While CRISPR and drugs dominate the screening...
Antibodies 101: Monoclonal Antibodies

Type

Blog Post

... of Murine Monoclonal Antibodies by Hybridoma Technology. JoVE. https://doi.org/10.3791/54832 Lipman ...
7 Tips to Secure a STEAM Internship This Summer

Type

Blog Post

...Several days prior to the interview, inspect your technology, background, and lighting for a smooth performance...
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

Type

Blog Post

...plasmids available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids...
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

Type

Blog Post

...Cas domain compatibility and activity. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0453-z Additional...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

... can be especially important for base editing techniques, where Cas9 must be directed to a very specific...
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

Type

Blog Post

...a gBlocks® Gene Fragment from Integrated DNA technologies. Generally speaking, anyone using CRISPR/Cas9...
Plasmids 101: Yeast Vectors

Type

Blog Post

...proteins yet they still share many of the same technical advantages that come with working with prokaryotes...
Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

...first iteration of these workshops took place at Caltech in Jan 2018 and involved presentations and a handout...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

...strong in this one. After all, the system built to protect us now seems to attack us by releasing mitogenic...
Human Germline Editing Using CRISPR

Type

Blog Post

...achieved any end goal not available with current techniques like PGD. Reference Ma, Hong, et al. "Correction...
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

Type

Blog Post

...expression at any copy number in bacteria." Nature biotechnology 36.4 (2018): 352. PubMed PMID: 29553576. 2. ...
Seven Tips for Using LinkedIn as a Scientist

Type

Blog Post

...examples of concrete accomplishments in both the technical and “plus skills” areas. Include a professional...
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

Type

Blog Post

...methods such as patch-clamp electrophysiology, a technique that’s used to measure electric current of ion...
Supporting Rare Disease Research with the 2020 BeHEARD Awards

Type

Blog Post

...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate those...
Antibodies 101: Affinity Reagents

Type

Blog Post

...sensitive drug delivery systems. Elsevier Science & Technology Books. Bates A, Power CA. David vs. Goliath: ...
Viral Vectors 101: An Introduction to AAV

Type

Blog Post

...Towards Gene Therapy For Duchenne Muscular Dystrophy. InTech. doi: 10.5772/53023 Veldwijk, Marlon R., et al....

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