...tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with two ...what you’re comparing your sample to. For example, we use our negative control to compare exposure times...cannot be reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody...reliable (wouldn’t perform differently over time), compatible with the desired applications (such as immunohistochemistry...techniques on rodent brains and is expanding to more complex brain systems such as non-human primates and humans...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of ...

...is mediated via individual neurons. Neurons are composed of a cell body, with dendrites receiving incoming...tracing experiments, brain tissue is injected with compounds that intracellularly spread away from the site...distant brain areas. It is desirable for tracing compounds to outline neurons in the direction of information...proteins facilitate transport of neuronal tracing compounds and this is an area that requires further study... neuronal tracing methods Traditional tracing compounds either produce a colorimetric product or are fluorescent...health. Another limitation of traditional tracing compounds is that their diffusion across the synaptic cleft... These methods are less neurotoxic and better compatible with other neuroscience methods such as electrophysiological...

...more about the company’s program. Once you’ve found a few positions to apply for, compare and contrast ...eventually work at the company at which you’re doing your postdoc? Some companies, such as AstraZeneca,... open up. Other companies, such as Genentech, will not. The point is that each company has a unique approach...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... publish, but this can vary depending on which company you choose. If publishing is important to you, ...that your project will not involve proprietary compounds or information. Where publication is expected,...meetings. How do I get an industry postdoc? Many companies have industry postdoc positions, including AstraZeneca...

...these components are necessary to efficiently reconstitute a functional viral polymerase complex and utilize...proteins are provided in trans (see Other Important Components of EBOV Minigenome System). However, minigenomes...RNA viruses (reviewed in 2, 3). Other important components of the Zaire ebola virus minigenome system Similar...with the viral polymerase, which is formed by a complex of two proteins: the large (L) catalytic protein...-stranded RNA minigenome. The viral polymerase complex then recognizes the encapsidated minigenome as ... genes in this plasmid would be more difficult compared to if each gene were encoded in separate (and ...particular observation can be easily studied as each component can be examined independently. In addition, experiments...

...antibody can be used. In a competitive ELISA a multiwell plate is coated with a competing antigen (green).  The...antibodies are used.  Competitive ELISAs Scientists typically use a competitive or inhibition ELISA when...converts into a detectable product. Scientists compare the signal intensity of the sample to that of a... of ELISA are direct, indirect, sandwich and competitive. In a direct ELISA a reporter enzyme conjugated...sample, the less antibody available to bind to the competing antigen. In direct, indirect and sandwich ELISA... level of antigen in the sample whereas in a competitive ELISA the reporter signal is inversely proportional...molecules that may bind non-specifically to assay components and increase background staining. Direct ELISAs...

...issues in the past, or how completely different teams are tackling comparable conflicts. Is this level ...cooperation: avoiding, competing, accommodating, collaborating, and compromising. Though there may be a...Skills series, this post is not meant to be a comprehensive guide to Conflict Resolution. There are countless...conflict is actually critical in the business world. Companies and organizations that avoid all conflict can ...with whom you strongly disagree, is a critical component of conflict resolution. Having empathy does not...course many other types of conflict resolution. In compromise, you are trying to come to a consensus that all...parties will accept. If you are able to broker a compromise well, you can help everyone walk away from a ...

... homolog of the yeast TREX-2 mRNA export complex: components and anchoring nucleoporin. Lu et al. Plant...generally known as Bimolecular Fluorescence Complementation (BiFC). It can provide a qualitative or quantitative... or even single-particle tracking of the bound complex. There are so many FPs available, with many ways... In several cases an FP(1-10) fragment may be compatible with multiple FP(11) fragments, even from a different...because they are used with an already-available complementary fragment. Next, let's look at a round-up of...protein fragments for bimolecular fluorescence complementation analysis under physiological conditions. Shyu...

...so simple, and engineering complex cellular behaviors requires making complex manipulations to cells. For...assembly. Simply put, it assembles individual DNA components into larger units or circuits with functional...Transcription units (TUs), which are functional units composed of multiple parts transcribed together. They can...and end with a terminator. A simple TU could be comprised of just three parts: promoter, gene, and terminator...MoClo. By a similar principle, TUs can be further complexed into multi-TU constructs, known as multigene constructs...promoters, UTRs, genes, tags, etc.) into MoClo-compatible vectors. Level 1: Assembly of multiple Level ...interact with other proteins and with DNA, and these complexes regulate the transcription of specific genes. ...

...the key components of Nanoblades, how their editing efficiency and off-target effects compare to existing...the following four components into 293T cells. Figure 1: The key components of Nanoblades. Image... primary cells. Comparing Nanoblades to Existing CRISPR Delivery Methods When compared head-to-head with...for HDR editing and mouse-model generation. Key components of Nanoblades Nanoblades are a Cas9 RNP delivery...Nanoblades also had lower off-target effects when compared to DNA transfection of gRNA and Cas9 expression...of RNPs generally having lower off-target edits compared to DNA transfection.  Homology directed repair...helps form DNA and retroviral-particle or VLPs complexes. The team found that "all-in-one" Nanoblades facilitated...

...at major companies.  The majority of these meetings acted as a platform for significant company advancement...their 20’s who’ve built multibillion dollar tech companies using only laptops and some server space. But... find an extra layer of difficulty launching a company in the health sciences without those critical three... into commercial products and next generation companies.  We launched in the early spring of 2015 and ...Before even beginning the process of launching a company, you need to determine whether or not your idea...your idea, you will rarely succeed in launching a company without partners.  You need a dedicated team that...funding bodies shy away from one man teams.  The composition of the management team is more important than...