We narrowed to 954 results for: COMP

...with increasingly complex experimental conditions. The ability to construct more complex, multi-fragment...fragments in a single “one-pot” reaction to form complex, multi-insert modular assemblies that enable biosynthetic...as enzyme efficiency, stability, and buffer compatibility have placed practical limits on single- or two-step...enzyme cutting results in 5’ four base overhangs complementary to the overhangs of the assembled inserts. After...assembly Intermediate 5- or 12-fragment assembly More complex 24-fragment assembly Single insert cloning efficiencies...Sequencing of blue colonies showed the expected complete sequence for the lacI/lacZ genes (1), while sequencing...setting up assembly reactions in which a single component was purposefully omitted. Since any assembly is...

... Nextflow script. With our completed pipeline, we could adjust the compute resources when needed, for ... number of deposited plasmids increased, the accompanying sequencing data collection and analysis eventually...needed to transform the raw sequencing data into a complete plasmid sequence that would be ready for our QC...data pipeline is to perform several sequential computational tasks for which existing script(s) have been...containers are they are designed to ‘contain,’ in a complete package, all of the pieces of software that depend...is sequestered from other programs running on a computer, so it safely operates in isolation, and can be... be easily shared with others without any complicated setup. We created, tested, and debugged each of ...

...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). B) Protein...determine structures at comparable or higher resolution than using more complex protective methods or detergents...evolved optimizations for prime editing CHARM: A compact epigenetic silencer Recombinant antibodies for ...for low-abundance targets or natively isolated complexes. To address these limitations, the Funabiki Lab...linker histone H1.8 in Xenopus nucleosomes and in complex with its chaperone NPM2, but the technique should...tardigrade LEA, RvLEAMshort, protected samples and was compatible with the buffer additive MgCl2 and the crosslinking...other viral delivery methods, allowing all the PE components to be delivered in a single package. In a recent...

...tasks that can be accomplished quickly and things that will take longer. Completing the quick and useful...project they’re a part of, completing them will give me some sense of accomplishment. I will prioritize which...its utility) How much time it will take you to complete the task When picking which tasks to actually...will swiftly give you the wonderful feeling of accomplishment while the longer tasks will give you larger...larger payoffs later. Don’t be afraid to accomplish some quick but moderately useful tasks at the expense ...the long and highly useful tasks. The quick accomplishments will likely energize you. For example, let'...experiment. The first way, it will take you 3 days to complete the entire experiment, but you’ll have to spend...

...user to compare the emission spectra between all of the markers in the panel. This complexity index ranges...choice for overlapping markers. Compensation/reference control(s) Compensation or “reference” controls are...detector for PE. In order to correct for this, compensation has to be applied to “subtract” this spillover...up a small portion of that spectra to use for compensation.     Figure 1: The emission spectra... discern one marker from another. Instead of compensation, which relies on user adjustment, spectral flow...cytometry relies on “unmixing.” Unmixing uses a complex mathematical algorithm and single stained reference...series of histograms flipped onto its side and compressed into a heat map. BV421 peaks in V1, with secondary...

...which can compare up to five editing experiments at a time, and batch analysis, which compares hundreds...edits performed over multiple experiments and comparing the robustness, accuracy, and speed with existing...the accuracy of ICE analysis results was highly comparable (within r2=0.96) to that of NGS data. Advantages... functional KO of the targeted gene.   Analyze Complex Edits   Traditional Sanger sequencing-based analysis...analysis tools are unable to detect or analyze complex edits, such as those generated by multiplexed delivery... How ICE works   Following delivery of CRISPR components into target cells, genomic DNA from both edited...populations is PCR-amplified and Sanger sequenced. ICE compares these sequence traces to give a detailed analysis...

... the “damage” and producing a complete plasmid. Besides being compatible with any vector, the SLIC protocol...overhangs perfectly match up. They just need enough complementary ssDNA to hold the plasmid together, even if ...if there are gaps or flaps remaining. This incomplete plasmid looks like a homologous recombination intermediate...either by annealing a mixture of products or from incomplete PCR — although higher DNA concentrations are ... separate PCR products (middle branch); or by incomplete PCR, which skips the final extension step to ...single-stranded overhangs (right branch). The mixed PCR and incomplete PCR methods yield a minority of usable product...the linearized vector by T4 digestion; then the components are combined and annealed. The resulting recombination...

...tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with two ...what you’re comparing your sample to. For example, we use our negative control to compare exposure times...cannot be reproduced and projects that cannot be completed. A 2015 review analyzing and explaining the antibody...reliable (wouldn’t perform differently over time), compatible with the desired applications (such as immunohistochemistry...techniques on rodent brains and is expanding to more complex brain systems such as non-human primates and humans...polyclonal antibodies because of their immunoglobin component that can be produced in large “copies.” This homogeneity...monoclonal antibodies; Addgene collaborates with these companies to create and provide general availability of ...

...is mediated via individual neurons. Neurons are composed of a cell body, with dendrites receiving incoming...tracing experiments, brain tissue is injected with compounds that intracellularly spread away from the site...distant brain areas. It is desirable for tracing compounds to outline neurons in the direction of information...proteins facilitate transport of neuronal tracing compounds and this is an area that requires further study... neuronal tracing methods Traditional tracing compounds either produce a colorimetric product or are fluorescent...health. Another limitation of traditional tracing compounds is that their diffusion across the synaptic cleft... These methods are less neurotoxic and better compatible with other neuroscience methods such as electrophysiological...

...more about the company’s program. Once you’ve found a few positions to apply for, compare and contrast ...eventually work at the company at which you’re doing your postdoc? Some companies, such as AstraZeneca,... open up. Other companies, such as Genentech, will not. The point is that each company has a unique approach...people involved is staggering. The process is complicated. If you’re interested in the drug discovery process... publish, but this can vary depending on which company you choose. If publishing is important to you, ...that your project will not involve proprietary compounds or information. Where publication is expected,...meetings. How do I get an industry postdoc? Many companies have industry postdoc positions, including AstraZeneca...