We narrowed to 911 results for: TRO

...Noninvasive Controlling Stimulation ExogenousEasily controlled EndogenousNot easily controlled Unique...spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad control of whole...boundaries in neuroscience by granting targeted control over neuronal activity. While they serve similar...optogenetics differ are: Timing Targeted manipulation Controlling stimulation Invasiveness  Choosing the best ...Chemogenetics does not offer the same level of control over timing. Because chemogenetics relies on chemical... can reach target cells throughout the body. Controlling Stimulation Some studies rely on delivering reversible...stimulation to receptors. Optogenetics offers better control over these factors as the light source can be easily...

... or chaotropic salts. Capillary electrophoresis Quantifying DNA using capillary electrophoresis is ... using gel electrophoresis. Except, it’s smaller. And automated. Unlike gel electrophoresis, you only ...wavelength that they maximally absorb. The spectrophotometer measures these absorbances using UV-transparent...absorb best at 280 nm and organic compounds and chaotropic salts maximally absorb at 230 nm. The A260/A280...specific for double strand DNA (as compared to spectrophotometric methods that measure all nucleic acids). ... sample concentration for you. Agarose gel electrophoresis Though not the quickest way to quantify DNA...methods can’t tell you and you don’t need a spectrophotometer or a fluorometer to quantify DNA this way....

...have learned how to introduce genetic materials into cells in the lab. The introduction of foreign DNA or...research. There are multiple ways foreign DNA can be introduced into cells including transformation, transduction...plasmid of interest. Scientists can also use electroporation, the application of an electrical charge to...Transduction occurs when foreign DNA or RNA is introduced into bacterial or eukaryotic cells via a virus...Transduction is a common tool used by scientists to introduce different DNA sequences of interest into a bacterial...transduction. Scientists also use transduction to introduce foreign DNA into eukaryotic cells, like mammalian... process by which foreign DNA is deliberately introduced into a eukaryotic cell through non-viral methods...

...Finsterbusch, Michaela, et al. "Patrolling monocytes promote intravascular neutrophil activation and glomerular...same laser) cooperate to cause a higher-energy electronic transition in a fluorescent molecule (see figure...epifluorescence microscope and GFP fusion proteins controlled by promoters specific to the immune lineage, ...movements in real time. This technique was first Introduced to immunology by three papers in the journal ...of pathogen-immune cell interaction relied on in vitro studies where it was difficult to apprehend the ... The video above depicts the infiltration of neutrophils expressing LysM-GFP (green) into a draining lymph...infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating the bacteria...

...pouring in! While our lentiviral service is going strong, the AAV service has shown incredible growth this...EBFP-Cre Available in serotypes AAV1, AAV5, and AAV retrograde (the most popular form), this viral vector is...deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive...genes to various brain regions. You can partially control which cells are successfully infected by an AAV...serotype, but occasionally you may want to further control where your gene of interest is expressed.  A cell-type...cell-type specific Cre can provide you with that control in a modular way. For example, say you would like...contains a Cre-dependent mCherry cassette under the control of an otherwise ubiquitous promoter. You could ...

... for introducing one modification into the mouse genome, they are not commonly used to introduce multiple...opposed to ordering control mice from a stock center. This serves as a better control since the experimental...series, we covered techniques that can be used to introduce genetic modifications into mouse embryos. But ...knockout lines and Cre-lox lines, and how to properly control for genetically engineered mice in your experiment...mutations. This is because as more mutations are introduced into a single embryo, the likelihood that a mouse...genetic engineering should be used to separately introduce each mutation independently into different mice...not a waste. Best practice is to use littermate controls in your experiment - use mice that were bred in...

...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...promoters can be regulated via positive or negative control. Positive Repressible: An activator protein is ...pJKR-H-tetR from the Church lab uses this system to control expression of GFP.)  To turn this inducible system...system, Gossen and Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the...displays low promoter activity (~20% activity of the strong yeast promoter pTEF). As ethanol accumulates, it...interest to be expressed. These permit exquisite control of gene expression and tracing of gene expression... and Potter labs has refined the Q system and introduced the second generation QF2 activators. These activators...

...markers. Compensation/reference control(s) Compensation or “reference” controls are samples stained with a ...is not the same as your single color control. Reference controls: Beads or cells? Compensation beads are...mathematical algorithm and single stained reference controls to distinguish one fluorophore from another within...autofluorescence (you can see this in your unstained flow controls). Spectral flow cytometers include a tool called... or spectral flow. In spectral flow, reference controls tell the software to look for that exact signature...using conjugated dyes, it is critical that your controls and your sample antibodies are from the same batch... are a convenient tool to obtain a strong positive signal for a given fluorophore, as they contain a mixture...

...right negative control is critical to the success of BiFC experiments. The ideal control is a mutated variant... work. You will also need to generate negative control constructs — more on this later. For more on constructing... measure the fluorescence of a single FP. Your control construct may generate a modest level of fluorescence...intensity of your BiFC constructs to your negative control to determine whether your PIPs actually do interact...has been compromised (Kodama and Hu, 2012). Such controls are necessary because split FPs can associate,...fragments themselves cannot be used as negative controls because their expression level, stability, and...structural information, constructing appropriate controls may requires that you empirically test a number...

...delivering control shRNAs, GFP, and rtTA from the Tet-on system. The first two can serve as controls in a variety...virus, and quality control data for each of the materials we distribute. Quality control info can be found...The viral preparations undergo rigorous quality control testing at Addgene meaning they come ready made...used to activate expression of a gene under the control of the tet operator. AAV AAVs are the workhorses...are already available allowing you to chemically control neuronal activity while studying your phenotype...of interest. Optogenetic tools for similarly controlling neuronal activity with light will be available...determined by the serotype. You can use these as controls to determine if your AAVs are getting to the right...