We narrowed to 954 results for: COMP

...targets.  Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as...

...given antibody is available through different companies (Bandrowski et al., 2016). It’s also been well...creating an array of educational materials to accompany our antibody collection.  Addgene is sharing our...

...epitopes available if it is being selected out of a complex mix of proteins that have been extracted from a...Picking the Right Antibody  It can get even more complex if you’re looking for proteins that are interacting...

...to 266 μg/mL. The reaction only required three components: a master mix, the DNA of interest, and cell ...several pooled libraries: Cas13X and Cas13Y, two compact Cas13 proteins found in the metagenome datasets...

...CellTagging. By constructing lineage trees and comparing them with the gene expression changes of more ...CellTags for tagging two cell populations in a competitive transplant assay in the mouse intestine, demonstrating...

...the Rare Genomics Institute sponsors a global competition for researchers working on rare diseases, offering...paralysis (hemiplegia) that are often painful and accompanied by migraines. Those attacks can be brought on...

...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

...the cells within them work together or perhaps compete against each other. Getting two-color stimulation...three or four hour conversation. The brain is very complex and we've hosted hundreds of visitors in our lab...

...mutation that disrupts the function of YFG. You could compare this mutant fly to one lacking the mutation in ...wanted to express them within a specific neuronal compartment, you could express them individually or simultaneously...

...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ...

...   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes of non-target...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...

...would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation ...and colonization opportunities for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach...

...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

...techniques are continually being refined; it is completely plausible that scientists will eventually have...standardized process of good research practice: computer simulations, in vitro research, animal models,...

...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene...

...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

...and learn from them. I started at my previous company by taking on responsibilities such as mentoring...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

...resulting in a Cas9 with improved specificity. They compared on-site and off-site activity of Sniper-Cas9 to...et al. "Evolved Cas9 variants with broad PAM compatibility and high DNA specificity." Nature 556.7699 (...

... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...

...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

... Provide perspective on core values at another company/department Give more candid feedback and be able...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ...

...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...

...used along other thiol-containing acceptors in competition assays. “I believe that improved biosensors or...related to addiction and pain. Once these are completed, Hubbard will image these neurons using GCaMP7...

... but not necessarily at the same subcellular compartment as the opsin or chemogenetic receptor. This may...Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope...

... Addgene move from completing 2-3 large projects a year, on average, to completing 8-10 projects per year...

... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription...