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  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

  2. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    ...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

  5. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

  7. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ...

  10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

  11. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...

  16. The Future of Research Symposium Boston 2015

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    Blog Post
    ... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...Harvard’s Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory...

  17. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...

  20. Design Tips for Prime Editing

    Type
    Blog Post
    ...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...

  21. Hot Plasmids February 2024

    Type
    Blog Post
    ...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized...

  22. Hot Plasmids - October 2020

    Type
    Blog Post
    ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on...

  25. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

  26. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...

  28. Common Injection Routes in Mice

    Type
    Blog Post
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  29. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...plants, mammals, and insects. In this post, we will compare two of the most widely used systems, mammalian ... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...

  30. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  31. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  33. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  34. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...

  35. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  36. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  37. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  38. CRISPR Antimicrobials

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    Blog Post
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  39. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  40. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...
Showing: 721 - 760 of 927 results