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We narrowed to 1,023 results for: Cat

Showing: 721 - 760 of 1023 results

  1. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...This reduces the chance of integration at random locations that could cause deleterious effects. It is important...multi-plasmid system, where you have one AAV vector dedicated to expressing Cas9, while the other houses the...integration into CRISPR-induced DNA breaks. Nature Communications, 10(1). https://doi.org/10.1038/s41467-019-...

  2. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ... tumors, and moreso than other methods, the identification and isolation of rare cell types. In recent...complexity index ranges from 0-1, with a value of “0” indicating those markers are completely distinct from one...one another, and a value of “1” indicating those markers are identical and completely indistinguishable...

  3. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...These impurities cannot be removed during the purification step because they are inside the virus itself...very low concentration. So how do we make sure to catch all of these potential mishaps before distributing...Guerin et al., 2020).  In addition to genome identification, we developed custom open-access Python scripts...

  4. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...to rapidly punch out cell clones with sterile truncated pipet tips. Indeed, an experienced operator can...wiped down with 70% ethanol, and a box of sterile truncated pipet tips (USA Scientific #1011-8410) is also..., and making sure we always maintain a backup replicates of each clone in case of contamination. We do...

  5. Of Myc and Men

    Type
    Blog Post
    ...used in way more applications.   Fig. 1 Making a Protein Tag (Abridged) (1) Locate a promising monoclonal... by its antibody without additional chemical modification steps and when the epitope was within the tagged...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ... to be considered when choosing an FP: Unique categories of fluorescent proteins Photoactivatable / Photoconvertible... used are GECIs - genetically encoded calcium indicators (e.g. GCaMP). Others include: pHluorin & pHTomato.... al. (2010) “Fluorescent proteins and their applications in imaging living cells and tissues”  Physiol...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... time advances in human genetics led to the identification of several disease-causing genes, including... But it is the hard work and persistence of a dedicated team of scientists led by Dr. Jean Bennett (UPenn...-1250015778 7. Yu W and Wu Z., 2018. In vivo Applications of CRISPR-Based Genome Editing in the Retina...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...in bacteria and extracted with common protein purification methods. Read on to learn more about nanobodies..., Alexa Fluor 568, or Alexa Fluor 647 before application to the sample. Staining with this multi-color...PubMed PMID: 29263082 Find plasmids from this publication at Addgene. 2. Bates, M., Böhning, M.I., Chug...

  10. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...common antibiotic resistance genes and which applications are best (or worst) for each antibiotic. The...is expensive (relative to amp), and for some applications, the benefit it offers is marginal. Fortunately...antibiotic resistance ultimately comes down to your applications and needs. The more niche and specific your ...

  11. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    ...is only effective when sgRNAs target a specific location on the gene. For CRISPRa, sgRNAs were targeted...Calabrese pooled library and lentiviral prep here! Applications for the Root and Doench CRISPR libraries Overall...et al. "A protein-tagging system for signal amplification in gene expression and fluorescence imaging....

  12. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...be an obvious one, but don’t make things too complicated for yourself (especially if you are the sole ...sole event planner)! If you have a very intricate itinerary, there is a higher likelihood that something ...Delegate- it will save you time and sanity!    10. Communicate Make sure people know that there is something...

  13. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...detrimental as too little, resulting in MECP2 duplication syndrome or Rett Syndrome, respectively. One ...Efficient and Precise CRISPR/Cas9-Mediated MECP2 Modifications in Human-Induced Pluripotent Stem Cells. Front..., Hector RD, Cobb SR, Bird A (2017) Radically truncated MeCP2 rescues Rett syndrome-like neurological ...

  14. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...folding and stability, change post-translational modification sites and even affect protein function (Mauro... AP, Bilen B, Zavolan M (2013) Analysis of CDS-located miRNA target sites suggests that they can effectively...synonymous codon usage bias, and its potential applications. Nucl Acids Res 15:1281–1295 . https://doi.org...

  15. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ...2019). TREE is a real-time, fluorescent based identification system for the isolation of base-edited cell...to BFP Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular...Harris RS (2018) A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9...

  16. Bioinformatics at Addgene

    Type
    Blog Post
    ... from depositing laboratories and associated publications, but our quality control (QC) standards have...such as 96-well microplates, and thanks to our dedicated product and development teams, frequent updates...can be easily shared with others without any complicated setup. We created, tested, and debugged each ...

  17. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...experimental design and its potential for clinical applications. Specifically, efficacy rates of Cas7-11 were...place, while increasing the number of potential applications. But they weren’t done engineering quite yet... at the bench and potentially in therapeutic applications. The potential of CRISPR has yet to fully taken...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...vincristine, are regulated in the medicinal plant, the Catharanthus roseus (Figure 1). The ultimate goal of our ...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...expression method for analyzing gene function in Catharanthus roseus seedlings." Frontiers in plant science...

  19. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...          The artificially evolved adenosine deaminase catalyzes the conversion of a target ‘A’ to ‘I’ (inosine... optimize base editing efficiency based on the location of the ‘A’ relative to the protospacer adjacent...Choose a base editing platform depending on the location of your target base in the protospacer sequence...

  20. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ... potential control, and provide evidence that truncated gRNAs have improved specificity with only a small...toxicity in high-throughput screens." Nature communications 8 (2017): 15178. Pubmed PMID: 28474669. Pubmed...Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature biotechnology 32.3 (2014):...

  21. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...controlled by collections of neurons that are intricately wired into circuits through synaptic connections...photostable than GFP. In addition, antibody-based amplification of their signals is weak and increases background...more HA tags. 4. High-resolutions microscopy As indicated above, smFPs can be used with conventional microscopes...

  22. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...hesitation to ‘approach strangers’ and learn how to communicate outside my scientific expertise. Organizing a...will lead! Read our blog post about fostering communication in remote collaborations! Stay in touch: Maintaining...connection is to make sure you keep open lines of communication with colleagues who move on to new organizations...

  23. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...Microbes (bacteria included) are very skilled at the delicate art of survival. Existing virtually ubiquitously...of intentions. The take-home? Always take your medication as prescribed. How do we know which bacteria ... is passionate about health equity, science communication, and public health.     References 1. O'Neill...

  24. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...diverse group of ethnicities, cultures, genders, educational, and professional backgrounds. Sometimes this... information. It is quite possible that the clarifications lead to the story being scrapped or broken ...While writing the code, we may need additional clarification and again we collaborate with the Product Team...

  25. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... in flow cytometry. Based on the established applications of the original hybridoma monoclonals, we anticipate... and plasmids. To encourage collaboration and education in protein science, IPI hosts events and training...subtype-specific β1 integrin mechanics. Nature Communications, 13(1), 7471. https://doi.org/10.1038/s41467...

  26. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...setting. CRISPR is often used for animal germline modification, to repair or add in disease-causing mutations...that AAV-CRISPR does not cause obvious germline modification, an important finding given the controversy ...Blog Learn How CRISPR Could Be Used to Treat Cataracts and Cystic Fibrosis Read How Homology Directed...

  27. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...tests  Statistical tests fall into two general categories: parametric and non-parametric. In this blog ... exist somewhere within your data; it does not indicate which groups the differences exist between. For...that matter. However, this is oftentimes an oversimplification. If your independent variables are affecting...

  28. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...immune response to the development of tumors is a complicated and dynamic phenomenon that is interestingly ...effects, damage healthy cells, and often cannot eradicate metastasis or eliminate the probability of a relapse...based on their level of progression, anatomical location, characteristic (solid localized tumors or systemic...

  29. New Antibodies in the Lab Animation!

    Type
    Blog Post
    ...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...or quick refresher on — these common antibody applications.  Thanks for watching, and we hope you found...

  30. Addgene Launches Partnership with IPI

    Type
    Blog Post
    ...antibody collection, as well as access to IPI educational resources developed to support use of their antibody... Addgene also offers a growing collection of educational resources on our website and blog, including ...

  31. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    ...these tools over the years, the amount of CRISPR educational resources hosted on our website and blog have...variants. Next, we’ll look at various CRISPR applications (such as visualize, screen, activate, and repress...

  32. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    ...Additional resources on the Addgene blog:  Flies 101: Catching flies in the lab References Abril, U.N.O., et....et. al. (2022) A plasmid-based system for locating and containing escaped flies. Nature 4:1-22. https:/...

  33. New Videos: Addgene Lab Tips

    Type
    Blog Post
    ...With barcode tracking, you will always be able to locate materials quickly and easily. Learn more about ...Additional Resources on the Addgene Blog New Educational Resource: The Addgene Videos Page 5 Science Rap...

  34. Twenty Years of Sharing Science!

    Type
    Blog Post
    ...easy tech transfer process to our website and educational resources. We've got twenty years of experience... you know you can search for plasmids from a publication by pasting the paper's title into the Addgene...

  35. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    .... I’ve selected “seminal” studies for various categories, including CRISPR gene-editing, the foundations...synthetic biology, DNA assembly, and various applications. I think these papers are good starting points...

  37. Podcast: A Malawian Professor's Path to Biotech Research

    Type
    Blog Post
    ...Born to a family in Malawi that pushed science education, Kingdom Kwapata grew up to be one of the best... 1:01-3:48 : Kingdom Kwapata's background and education 3:49-4:35 : Kingdom on opportunities to go into...

  40. CRISPR Cheat Sheet

    Type
    Blog Post
    ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...all sequences. However, prime editing and the identification of other Cas proteins continue to expand the...
Showing: 721 - 760 of 1023 results