We narrowed to 801 results for: MAK

1. Hot Plasmids - October 2020

   Type

   Blog Post

   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

2. Corralling and Cornering the Goldilocks Antibody

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   Blog Post

   ... one place... well, lucky for you one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and...

3. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

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   Blog Post

   ...Nanoblades? They’re easily produced in a BSL2 lab, making them a versatile and accessible genome editing ...

4. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   ...produced various resources for the worm community that makes it easier to view and explore the worm. These resources...

5. Fluorescent Proteins 101: Introduction to FRET

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   Blog Post

   ...reviews have collated data from various papers to make the decision easier. 1. Müller, Sara M., et al. ...

6. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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   Blog Post

   .... At the same time, there is much opportunity to make headway on long-term efforts to better account for...

7. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ... it’s time to move ahead with your infection and make your stable cell line. While we’ve all experienced...

8. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ... of cytosine to 5-methylcytosine in CpG islands makes  genes less transcriptionally active. The role ...

9. Hot Plasmids: Winter 2025

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   Blog Post

   ...and more photostable than fluorescent proteins, making them ideal for single-molecule fluorescence techniques...

10. Common Injection Routes in Mice

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    Blog Post

    ...that they don’t always require needles. If needles make you cringe or your experiment will allow you to ...

11. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ...of rAAV production.  Of course, you will want to make sure that your final stocks only contain rAAV. To...

12. Antibodies 101: Flow Compensation

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    Blog Post

    ...will ‘look’ similar on your flow plot. This will make it difficult to properly gate your desired cell ...

13. Plasmids 101: Degron Tags

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    Blog Post

    ...degron will behave with a new protein, but you can make an informed choice! References and Resources References...

14. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ...if you’d like to use homology directed repair to make precise edits or knock-ins. Editing efficiency decreases...

15. Fluorescent Tagging of Endogenous Genes with SapTrap

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    Blog Post

    ... protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for...

16. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...ability to differentiate into multiple cell types makes them useful models for developmental processes and...

17. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

18. Plasmids 101: Protein Expression

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    Blog Post

    ...translated at the ribosome into polypeptide chains that make up the primary structure of proteins. Most proteins...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post

    ...target cells (Beatty et al., 2012). These features make AdV an attractive candidate for in vivo delivery...

20. The Scientific Conference Poster Session: Tips for Success

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    Blog Post

    ...interested in viewing which has drawn a large crowd, making it difficult to see the actual poster or interact...

21. Google Forums Round Up: First Impressions of NgAgo

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    Blog Post

    ...is required for NgAgo optimization before we can make any major conclusions: That said, many people are...

22. An Introduction to Adenovirus

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    Blog Post

    ...process – such as the viral DNA polymerase – it makes sense that vectorization strategies include creating...

23. Getting to Know Expansion Microscopy

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    Blog Post

    ...synaptic transmission, are right around 250 nm, making it difficult to study the inner workings of the...

24. Plasmids 101: Secondary Nanobody Toolbox

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    Blog Post

    ...domain proteins, they can be expressed in bacteria, making nanobodies a recombinant and renewable anti-IgG...

25. CRISPR 101: RNA Editing with Cas13

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    Blog Post

    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...

26. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

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    Blog Post

    ...added log-normal noise to each of the terms which make up our distribution, resulting in the following ...

27. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...number of administrative and practical decisions to make. How often will you hold it? How many people do ...

28. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    Blog Post

    ...ve built a new and improved search engine to help make this task a faster, easier, and frustration-free...

29. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ... Using this technique, researchers have begun to make important discoveries in various biological fields...

30. Simplify Cloning with in vivo Assembly

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    Blog Post

    ...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

31. AAVs in Retinal Gene Therapy

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    Blog Post

    ...started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A better vector...

32. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...decide which ones to use? Here are some tips for making those decisions: Always always always look at ...

33. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

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    Blog Post

    ... DNA compaction and the structural proteins that make it possible, see the appendix DNA is a 3D fractal...

34. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...

35. Your Lentiviral Plasmid FAQs Answered

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    Blog Post

    ...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

36. Plasmids 101: Broad Host Range Plasmids

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    Blog Post

    ...//doi.org/10.1111/1574-6968.12241  Keen, N. T., Tamaki, S., Kobayashi, D., & Trollinger, D. (1988). Improved...

37. Antibodies 101: Reading a Flow Plot

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    Blog Post

    ...often looks like the graph on the left, it might make more sense once you realize events are plotted on...

38. Plasmids 101: Gateway Cloning

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    Blog Post

    ...gene! STEP 2: Generate an Expression Clone When making the expression clone, it is important to choose...

39. Mesothelioma - Causes, Symptoms, and Treatment

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    Blog Post

    ... long latency period, and rarity of mesothelioma make it different from many other types of cancer. Mesothelioma...

40. 5 Reasons to Use Reddit for Science Communication

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    Blog Post

    ...until 5 pm). The ability to see the questions first makes Reddit a great platform for those who aren’t so ...