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Showing: 721 - 760 of 1055 results
  1. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...
  2. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...
  3. Let There Be LITE Plasmids

    Type
    Blog Post
    ...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...
  4. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...
  5. Modulate the Activity of 17 Signaling Pathways with One Kit!

    Type
    Blog Post
    ...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...
  6. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are open to feedback...
  7. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...
  8. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year.  In September...
  9. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them...
  10. Are Hybrid Guide RNAs Right for Your CRISPR Application?

    Type
    Blog Post
    ...sequence, which doesn’t contact genomic DNA at all. Instead, the Cas protein specifically recognizes its folds...some hybrid guides increased off-target editing instead of decreasing it (Whittaker et al., 2025). However...
  11. New Viral Vectors - Fall 2024

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    Blog Post
    ...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ...
  12. An Addgene Summer Internship

    Type
    Blog Post
    ...toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ...
  13. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ...demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone...
  14. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...
  15. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening...
  16. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...
  17. Advice for Choosing a Research Project

    Type
    Blog Post
    ... your professional brand. It offers colleagues insight into your interests and will determine how you ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...
  18. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...
  19. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    ...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain...exclude people outside of the original recipients. Instead, it targets a specific audience - giving consistency...
  20. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...embryogenesis. My interests, though, have proven unruly. Instead of settling down into a singular focus on molecular... general terms on a couple of very broad topic domains, but each day brings in new and surprisingly diverse...
  21. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...Instead of spending time and money producing virus from select vectors in the repository yourself, you...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can...
  22. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...company advancement. Be relentless The world is against you.  As I mentioned, you start at a disadvantage...
  23. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...
  24. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...
  25. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...
  26. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...
Showing: 721 - 760 of 1055 results