We narrowed to 1,055 results for: ins;

...loads of inspiration for the current and the next generation of microbiologists. If you got inspired to produce...

... technologies were without controversy, and it remains to be seen how popular any one of them will become... though a little confusing to the uninitiated, contains much of the information needed to understand how...

...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...

...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...

...the Genetic Perturbation Platform at the Broad Institute, John Doench. A genetic screening project can ...still offering the potential for true biological insight. Pooled and individual plasmid formats A first ...

...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are open to feedback...

...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...

...Maintaining Institutional Knolwedge and Continuity - SITN's key technique for retaining institutional knowledge...

...CRISPR/Cas9 for targeted genomic deletions We were inspired to produce intrachromosomal deletions based on...copy at a given locus may be useful to study haploinsufficiency. Subsequent modifications at the remaining...

... own.” “Someone to act as your ‘cheerleader’ – inspire you.” “Also a mentor can give you realistic expectations...mentor does all of these things and more. It is instructive (and entertaining) to review this list of bad...

...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year.  In September...

...Kotelevtseva N, King K, Davis JR, Mullins LJ, Chapman KE, Dunbar DR, Mullins JJ. Nucleic Acids Res. 2007;35...

...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them...

...sequence, which doesn’t contact genomic DNA at all. Instead, the Cas protein specifically recognizes its folds...some hybrid guides increased off-target editing instead of decreasing it (Whittaker et al., 2025). However...

...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ...

...toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ...

...ARGs) are engineered gene clusters that encode proteins that form bacterial gas vesicles. These ~200nm... plasmids for in vivo imaging with fluorescent proteins Browse luciferase plasmids Find fluorescent protein...

...demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone...

...for example, cutting open a heart to look at the inside. We do a lot of interactives and videos, as well... Keown, a staff writer at the non-profit Fred Hutchinson Cancer Research Center. And make sure to subscribe...

...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...

...one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening...

...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

... your professional brand. It offers colleagues insight into your interests and will determine how you ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...

...libraries In surface display, proteins of interest are fused to anchoring proteins to be expressed on the surface...

...sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain...exclude people outside of the original recipients. Instead, it targets a specific audience - giving consistency...

...recombinase competition Article contributed by Aliyah Weinstein Sparse Predictive Activity through Recombinase...protein fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the...

...natural or synthetic, the researchers looked at intrinsic properties (GC content or rare codon percentage...not rare codons and many genes where there are instances of rare codons, sometimes intentionally modulating...

...kits designed for use in K-12 and college-level instruction. Six of the kits, known collectively as Greening... protocols and supporting materials such as instructional videos and datasheets. The remaining kits were...

...CRISPR than just designing a repair template that contains your desired edit, and I want to make sure that...long ssDNA template to increase efficiency of insertion and gene replacement. Care to share your favorite...

...embryogenesis. My interests, though, have proven unruly. Instead of settling down into a singular focus on molecular... general terms on a couple of very broad topic domains, but each day brings in new and surprisingly diverse...

...Instead of spending time and money producing virus from select vectors in the repository yourself, you...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can...

...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...company advancement. Be relentless The world is against you.  As I mentioned, you start at a disadvantage...

...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...

...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...

... Figure 1: Can you draw Spongebob from these instructions without knowing you're suppose to draw Spongebob..., test out the technology before the conference begins.“There were some concerns of having a stable internet...

...reading through a paper to find that there is insufficient information about which reagents were used? ... options using the different PID formats The instructions for how to cite any Addgene plasmid in your ...

... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...