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We narrowed to 898 results for: plasmid

Showing: 361 - 380 of 898 results

  1. CRISPR 101: Mammalian Expression Systems and Delivery Methods

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    Blog Post
    ...add a tag, you will also need to deliver a donor plasmid or single-stranded DNA oligonucleotide, so you ...generate stable cell lines. Packaging and Envelope plasmids provide the necessary components to make lentiviral...for in vivo viral delivery. Cas9 mRNA and gRNA Plasmids containing gRNA and Cas9 are used in in vitro ...efficiency of CRISPR delivery. There are a few plasmids at Addgene that have been published as CRISPR ...User) Resources on Addgene.org Browse CRISPR Plasmids for Mammalian Systems Check out our CRISPR Guide...

  2. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

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    Blog Post
    ...to elute and store your plasmid DNA Learn about the different grades of plasmid DNA preps Resources on...studies and is not used as much for the standard plasmid prep. Diphenylamine method Another absorbance based...Addgene.org Visit the Molecular Biology Reference Find plasmids for your research Browse protocols for molecular...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...The principle of Tetbow In modern neuroscience, plasmid or viral vector-mediated gene delivery is more ... use Tetbow, you can introduce the four Tetbow plasmids (Addgene #104102 - #104105) using in utero electroporation...Tips and troubleshooting your Tetbow experiment Plasmid concentrations and AAV titer - The XFP genes have...achieve the highest color variations. Adjust the plasmid concentrations or AAV titer for the best results...

  4. Antibodies 101: Chimeric Antibodies

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    Blog Post
    ...been cloned into plasmids. Because their sequences are known and encoded on a plasmid, they can be easily...recombinant antibodies. Recombinant antibodies are plasmid-based and are created synthetically or derived ...regions of a specific antibody are cloned into a plasmid backbone containing all constant regions, just ...

  5. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...ll work with you to get the word out about your plasmids and their potential educational uses. We believe...30083609. PubMed Central PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional ...developmental biology Use the pathways over time plasmids to engage students in functional genomics research...out our molecular biology reference pages Find plasmids from the BioBits BrightTM publication here ...

  6. How Do I Place an Addgene Order Using a PO Number?

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    Blog Post
    ...of information about how to deposit plasmids, store your plasmids or viral items, technical support, and...your favorite store is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene,...

  7. Why Do I Need an MTA Anyway?

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    Blog Post
    ...for material transfers. When Addgene entered the plasmid sharing space, we heard over and over again that...with the UBMTA result in quick approvals, so your plasmid requests can get to your lab faster. For Everybody...reference pages. So go ahead and use Addgene plasmids in your experiments. You know that they’re properly...

  8. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  9. Addgene's Cancer Collection Pages Connect the Research Community

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    Blog Post
    ...generation of mutant cell lines. Further, for each plasmid in the kit, the transcript most commonly found ...initiative to curate our collection of cancer-related plasmids such that each is linked to a known major pathway...pathway. Now, using our new Cancer Research Plasmids and Resources page, investigators may select a pathway...investigator to a list of all available Addgene plasmids he or she can use to study it (as seen in the ...Visit our Cancer Collection Pages Deposit Your Plasmids for Studying Cancer Browse Our Other Collections...

  10. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK.Cas9...looking for a broader range of CRISPRs plasmid tools, find more plasmids, CRISPR technology guides, FAQs, and...description of their experimental protocol. The three plasmids which have been deposited to Addgene are: pAdSh.PGK.Cas9...

  11. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...specific mutations in your plasmids Use REPLACER Mutagenesis to mutate your plasmid Resources on Addgene.... targeted mutagenesis experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo...Browse our CRISPR guide pages Find bacterial CRISPR plasmids ...

  12. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...: 1262. PMID: 23232392 PMCID: PMC3535334 Find plasmids from this paper at Addgene. Takai, Akira, et ...4352-6. PMID: 25831507. PMCID: PMC4394297 Find plasmids from this paper at Addgene. Suzuki, Kazushi, ... 13718. PMID: 27966527 PMCID: PMC5171807 Find plasmids from this paper at Addgene. Additional Resources...All Our Fluorescent Protein Resources Find FRET Plasmids Find Empty Backbones with Fluorescent Protein ...

  13. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    ...Addgene.org Plasmid Pooled Libraries Pooled library amplification Zhang Lab CRISPR Plasmids The Human TFome...controls. Alternatively, you can order individual plasmids from the library to further investigate specific... of the collection, we’re releasing individual plasmids in batches. If there’s something you’d like that...

  15. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post
    ... great collection of ready-to-use rAbs and rAb plasmids!). Since recombinant antibodies conveniently allow...antibodies can be produced through a single or dual-plasmid transfection, depending on whether the heavy and...and light chain genes are contained to one plasmid or if they’ve been separated onto two. You’ll first...first transfect your cells with your plasmid(s), and then wait 7-14 days (in mammalian cells) to collect ...on the Addgene blog Introduction to Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies...

  16. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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    Blog Post
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  17. Editor's Choice, September 2016

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    Blog Post
    ... Tips for Writing a Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates...Kinome Libraries Available: Pooled and Individual Plasmid Formats Better Dyeing Through Chemistry & Small...Addgene supporters who spoke with me and deposited plasmids on this trip. Here’s to hoping that October is...

  18. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic microbes...granules. We overcame this problem by engineering a plasmid which contained cold tolerant chaperones (the Cpn...pMBP-LbCas12a and pC013-Twinstrep-SUMO-huLwCas13a plasmids from Addgene, which would potentially allow a ...biology blog posts Resources on Addgene.org Find plasmids for your synthetic biology experiment Read our...

  19. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    ...that Addgene provides is an open repository of plasmids and other necessary information that’s required...the collection, storage, and distribution of the plasmids and their ability to identify and fulfill a need... helping the scientific community manage those plasmids so scientists aren’t bogged down with distributing...continuing to look at providing other things besides plasmids to the scientific community through Addgene’s ...

  20. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Engineered   677 61 AkaLumine ATP No Common uses for plasmids expressing luciferase Luciferase reporter assays... partnered with Promega to offer their Promega Plasmid Collection, including tools using NanoLuc®, NanoBiT...collection page Promega Plasmid Collection Fluorescent Proteins 101 ebook ... plasmids 101...
Showing: 361 - 380 of 898 results