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We narrowed to 113 results for: PIP

Showing: 11 - 20 of 113 results
  1. Deep Dive: qPCR

    Type
    Blog Post
    ... into your data. Try different types of pipettes and pipetting methods to find what’s most consistent ...plate in a way that will tell you if you have pipetting errors on the edges of the plate. Compare the ...multiple configurations can help you identify pipetting or evaporation errors. And if time allows, you...BioRender.   Passive Reference Dyes Whatever your pipetting method, some variation will occur. A passive reference...
  2. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    ... MiSeq, the data is imported into our assembly pipeline that runs with Seqera Nextflow. The workflow converts...such as SPAdes, Shovill, apc, and NOVOPlasty. The pipeline contains three de novo assemblers, so if one fails...assembles the raw data.  Addgene’s de novo assembly pipeline usually generates the correct plasmid sequence...methods like automation. All assemblies from our pipeline are now run through an auto-align process to match...
  3. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    ...source workflow orchestration software for data pipeline processing (Nextflow), cloud infrastructure, and...collection of scripts evolved into a single, organized pipeline and accelerated the processing of our sequencing... has used Nextflow to improve and redesign our pipeline. In it, they break down the unique challenges ...
  4. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...scientists washed and reused materials such as glass pipettes or inoculation loops. While single-use is now ...a single use. So how about washing and reusing pipette tips in a high-throughput and efficient way? Grenova... devices that are compatible with a variety of pipette tips. This is part of a cultural change in how ...
  5. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...screens together and expedited the drug discovery pipeline. Basics of drug discovery Present drug discovery...potential targets by CRISPR can accelerate the drug pipeline. High-throughput CRISPR screens can be used to... aids in multiple stages of the drug discovery pipeline.   CRISPR and drugs in the future While ...
  6. Lab Automation at Addgene

    Type
    Blog Post
    ...as a device fits on the deck, and it’s easy to pipette samples in and out of it, you can set up a procedure...using electronic multichannel and single channel pipettes. These can speed up any project in which you need... to do a lot of aliquoting. Simply program the pipette with how much total sample you need and the volume...
  7. A Look at Addgene's QC Process

    Type
    Blog Post
    ...of a reference sequence) using a bioinformatics pipeline developed at Addgene. The assembled plasmid sequence...request. Otherwise, they continue through the QC pipeline to resolve any issues.  Catching and addressing...arise due to errors in the sequencing and assembly pipeline. There are regions of plasmids that are particularly...
  8. Droplet Digital PCR for AAV Quantitation

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    Blog Post
    ...Have a dedicated bench with a dedicated set of pipettes for ddPCR set-up. Prepare the master mix in a ...the bench and all consumables with 10% bleach.  Pipette slowly If you are using a manual droplet generator...automated droplet generator, you should avoid pipetting too quickly when making your virus dilutions. ...
  9. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ... to poor lab techniques. These include reusing pipettes for multiple cell lines rather than using disposable...aerosols that are generated (while talking or pipetting) during working in the laminar airflow are also... are tightly packed with seals. Use disposable pipettes to avoid cross-contamination. Make sure you keep...
  10. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    ... or if you get a little overzealous with your pipetting. Another is the possibility of carrying over contaminants...practices for the lab. Plastic consumables (including pipette tips, plates, and yes those purification columns...buffer bottles Fewer plastic consumables Fewer pipette tips needed in workflow Reduced waste from ...
Showing: 11 - 20 of 113 results