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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,011 results for: RING;

Showing: 11 - 20 of 1011 results

  1. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year Boston... space here at our new location to grow. We are hiring again and getting ready to embark on new exciting...distributed in 2018 and we are poised for more offerings. The viral vector team is publishing research,...We must keep spreading the word about materials sharing, but we don’t have to worry about getting support...False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False, width='1280', height...

  2. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...chromosomal DNA.  Plasmids are said to be under stringent control when they depend on the host cell’s initiation...consider the replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...replication for the bacterial chromosome and for stringently controlled plasmids. Binding of IHF (green) redistributes... cell growth or even kill the host cell. For stringently controlled plasmids, replication is tightly coupled...number.  Let’s consider pSC101. This plasmid is stringently controlled and has a low copy number. Like the... necessary. Iterons are not the only tool to stringently regulate plasmid replication. For example, the...Comparison of replication regulation mechanisms of stringently controlled plasmids pSC101 and ColIb-P9. pSC101...

  3. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...also a story of community, cooperation, and sharing. Sharing this story is a truly special way to celebrate...As we celebrate twenty years at Addgene, we are sharing stories of our repository’s positive impact on ... that developed CRISPR tools were committed to sharing them with the rest of the community. They did this...the usefulness of popular CRISPR tools is both enduring and rapidly recognized.   Where is the CRISPR ...appropriate MTAs and customs forms. Of course, ordering the plasmids is the easy part; using them can ... resources on the Addgene blog Twenty Years of Sharing Science  ...

  4. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...circuit by integrating neuronal signalling and triggering release of the stress hormone norepinephrine ...Cre-expressing AAV-5 into the DRN of a transgenic mouse harbouring a Cre-dependent IL-1 beta overexpression cassette...lit (risky) and a dark (protected) area. When measuring the time spent in a particular part of the apparatus...levels within the hippocampus and frontal cortex during depression, yet there have also been contradictory...

  5. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ... of proteins without the challenges typically occurring with a fluorescence-based approach. These challenges...vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic traits, facilitating...EGFR. This method offered kinetic, non-invasive monitoring of receptor dynamics at the cell surface. Find...HiBiT-EGFR and EGFR-HiBiT Fusion Vectors at Addgene! Measuring messengers: growth factors Growth factors present...) utilized HiBiT to address these challenges, exploring the binding kinetics of the VEGF-A and the VEGFR2...

  6. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...preparation steps of production, purification, and titering. Our newest viral vector service, AAV Packaged...

  7. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...many countries, import and export regulations, differing customs processes, and the distances the packages...specific to that country. ‌For example, when we began offering temperature-sensitive materials, we noticed a ...shipments arrived in India at room temperature. Partnering with our distributor allowed us to ensure that...shipments weren’t able to clear import control. Partnering with a distributor allowed us to share materials...scientists in China. They were also able to help with sharing information through the use of popular Chinese ...solutions aren’t enough to ensure accessibility, partnering with distributors who have expertise in a specific...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...imagination of most of the authors during this period.  Genetic Engineering Era: Pre-CRISPR  Having visualized...associated with genetic engineering in the recent SciFi movies. Rampage (2018), starring Dwayne “The Rock” ...California, San Diego. The revolution in genetic engineering techniques is a speculation of yesteryear which...but the common themes that these authors were exploring were space and robots. Warping to stars in space...devise methods to manipulate it. The term genetic engineering itself is attributed to SciFi writer Jack Williamson...s Island. In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...these corporations would extrapolate genetic engineering to exploit human beings. Ridley Scott directed...
Showing: 11 - 20 of 1011 results