Plasmids 101: TOPO Cloning
Type
Blog Post
... Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):10104-8. PubMed PMID: 1658796. PubMed Central PMCID: PMC52876...
We narrowed to 75 results for: 104
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Using CRISPR/Cas9 to Edit Disease Out of the Genome
TypeBlog Post...Current genetics. https://doi.org/10.1007/s00294-019-01040-3 3. Khatibi et al. 2021. “CRISPR Genome Editing... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post... New calcium sensors Plasmid Serotype Name 104496 AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322 ... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post... PubMed PMID: 24917028. PubMed Central PMCID: PMC4104587. György, Bence, et al. "Rescue of hearing by ... -
Viral Vectors 101: Biosensors
TypeBlog Post...Biosensors. Essays Biochem. 2016 Jun 30;60(1):1-8. DOI: 10.1042/EBC20150001 Leopold, A. V., Shcherbakova D. M.,... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post...enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041-SpCas9. The resulting base editor was named ABE8e... -
Plasmid Tools for Microbiome Studies
TypeBlog Post... PubMed PMID: 30131493. PubMed Central PMCID: PMC6104080. Resources on the Addgene Blog Find microbiology... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...), Antibodies: a Laboratory Manual, 2nd Edition. 2104. Cold Spring Harbor Laboratory Publications, New... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...analysis. Science 285, 901-906. (1999). PubMed PMID: 10436161. 7. Dujon, B. Yeast evolutionary genomics. Nat... -
Plasmids 101: Terminators and PolyA signals
TypeBlog Post... PubMed PMID: 10074136. PubMed Central PMCID: PMC104046. Wodrich H, Schambach A, Kräusslich H-G. Multiple... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post... 13(16), 1935–43. https://doi.org/10.1089/10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, ... -
Viral Vectors 101: Producing Your rAAV
TypeBlog Post... 15(7), Article 7. https://doi.org/10.1089/1043034041361262 More resources on the Addgene blog Parts of... -
Pooled Library Amplifications
TypeBlog Post...Activation Human Genome-wide CRISPRa-v2 Libraries 104,540–209,080 20 bp 100 ng Liquid Liquid Culture... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...Interactions by Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog Post...Society of Microbiology, 2015. 647-667. PubMed PMID: 26104363. Additional resources on the Addgene blog Learn... -
An Introduction to Adenovirus
TypeBlog Post...doi.org/10.1128/JVI.73.8.6930-6936.1999. PMID: 10400791. Kafri, T., Morgan, D., Krahl, T., Sarvetnick,... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog Post... by biotinylated dCas9." Cell 170.5 (2017): 1028-1043. PubMed PMID: 28841410. Qi, Lei S., et al. "Repurposing... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post.... PubMed PMID: 9971816. PubMed Central PMCID: PMC104478. 5. Mühlberger, Elke. "Filovirus replication and... -
AAV Titers: Where Do They Come From And What Do They Mean?
TypeBlog Post... AAV-2." Gene therapy 6.7 (1999). PubMed PMID: 10455443. 3. Hindson, Benjamin J., et al. "High-throughput... -
Hot Plasmids: February 2026
TypeBlog Post...#50459) were packaged into AAV using Old (Addgene #104964) or New (Addgene #232922) pAAV2/5. Average + standard...