We narrowed to 167 results for: 11

1. Synthetic Photobiology: Optogenetics for E. coli

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   Blog Post

   ...Synth Biol. 2014 Nov 21;3(11):820-31. doi: 10.1021/sb500273n. Epub 2014 Oct 11. PubMed. Find the plasmids...

2. Typing CRISPR Systems

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   Blog Post

   ...effector was developed, called Cas7-11. Despite being in Class 1, Cas7-11 is a single protein effector and...CRISPR–Cas systems. Nature Reviews Microbiology, 13(11), 722–736. https://doi.org/10.1038/nrmicro3569 Makarova...targeting with the single-protein CRISPR effector Cas7-11. Nature, 597(7878), 720–725. https://doi.org/10.1038...

3. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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   Blog Post

   ...keratinocytes. Nat Biotechnol, 2008. 26(11): p. 1276-84. PubMed PMID: 18931654. 11. Loh, Y.H., et al., Reprogramming...generation of human cardiomyocytes. Nat Methods, 2014. 11(8): p. 855-60. PubMed PMID: 24930130. PubMed Central...induced pluripotent stem cells. Stem Cell Rev, 2015. 11(2): p. 242-53. PubMed PMID: 25578634. PubMed Central...

4. Sharing is Caring: But How to Distribute Open Hardware?

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   Blog Post

   ...the OpenFlexure microscope," Biomed. Opt. Express 11, 2447-2460 (2020) Hongquan Li, Deepak Krishnamurthy...open standard for microscopic imaging. Nat Commun 11, 5979 (2020). https://doi.org/10.1038/s41467-020-...

5. The 12 Days of CRISPR: 2021

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   Blog Post

   ...updated CRISPR 101 e-book (to help with the other 11 presents!)  Can't get enough of CRISPR? Neither can...

6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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   Blog Post

   ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038/s41467-020-15731-w Wang... ERF Transcription Factor Gene OsERF922. PLoS ONE 11:e0154027 . https://doi.org/10.1371/journal.pone.0154027...

7. CRISPRainbow and Genome Visualization

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   Blog Post

   ...with gene editing. CRISPRainbow requires very short, 11-mer gRNAs, which do not induce genome editing. If...editing, activation and repression.” Nat Methods. 12(11) (2015):1051-4. PubMed PMID: 26344044. PubMed Central...

8. Plasmids 101: Using Transposons in the Lab

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   ...site: TNA(C/G)TTATAA(G/C)TNA. Tol2 can deliver 10-11 kB to mammalian cells without decreased efficiency...payload for lentivirus). Large cargos, such as the ~11 kB cDNA for dystrophin, the gene mutated in muscular...Transposons: Nature and Applications in Genomics. CG 11:115–128. https://doi.org/10.2174/138920210790886871...

9. Educational Planarian Tools for Teaching Developmental Biology Techniques

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   Blog Post

   ...Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids to investigate...on the WISH technique. The expression patterns of 11 different planarian genes (all of which are available...

10. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post

    ...coefficient of a protein. Protein Sci. 1995 Nov;4(11):2411-23. doi: 10.1002/pro.5560041120. PMID: 8563639...particle formation. J Pharm Sci. 2015 Feb;104(2):602-11. doi: 10.1002/jps.24259. Epub 2014 Nov 21. PMID: ...

11. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post

    ...high generalization performance. Science Advances, 5(11), eaax9249. https://doi.org/10.1126/sciadv.aax9249...repair determinants. Nature Biotechnology 2023, 1–11. https://doi.org/10.1038/s41587-023-01678-y Li, X...

12. Plasmids 101: Choosing an Antibiotic Resistance Gene

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    Blog Post

    ...cytoplasm. Microbial Cell Factories, 11, 56. https://doi.org/10.1186/1475-2859-11-56 Trastoy, M. O., Defais, M...

13. Getting to Know Expansion Microscopy

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    Blog Post

    ...Ten-fold Robust Expansion Microscopy (TREx). Elife 11:e73775. https://doi.org/10.7554/elife.73775  Dani...proteins in their ultrastructural context. Nat Commun 11:3850. https://doi.org/10.1038/s41467-020-17523-8 ...

14. Lentiviral Vector Uses and Overview

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    Blog Post

    ...Nguyen M, Trono D, Naldini L. J Virol. 1998 Nov;72(11):8463-71. PubMed PMID: 9765382. PubMed Central PMCID...Sanjana NE, Shalem O, Zhang F. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID: 25075903. PubMed Central PMCID...

15. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

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    Blog Post

    ...proteins. Our studies and those of others (5, 6, 7, 10, 11) highlight the very real consequences of misfolded... PMID:15557121. Pubmed Central PMCID: PMC2172584. 11. Jokitalo, Eija, et al. "Golgi clusters and vesicles...

16. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...produce Murray’s clear solution.  Murray's clear (11) was mostly used to study the development of vertebrate...M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983–1995. Pubmed. K. Chung, J. Wallace, S.Y. Kim...

17. An Interview with Connie Cepko - Gene Therapy, Plasmid Tools and Insights to Success

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    Blog Post

    ...Activatable Tools Being Developed in the Cepko Lab 35:11-40:54 : Professor Cepko's Lab Management Style and...

18. Plasmids 101: Modular Cloning Applications and Kits

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    ... Cerevisiae.” ACS Synthetic Biology, vol. 12, no. 11, American Chemical Society (ACS), 6 Nov. 2023, pp...Toolbox for Plants.” ACS Synthetic Biology, vol. 3, no. 11, American Chemical Society (ACS), 20 Feb. 2014, pp...Mammalian Cells.” ACS Synthetic Biology, vol. 8, no. 11, American Chemical Society (ACS), 5 Nov. 2019, pp...

19. AAV Titration by qPCR Using SYBR Green Technology

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    Protocol

    ... = 1.59 x 10 11 molecules/μL To obtain a solution at 2 x 10 9 molecules/μL: 1.59 x 10 11 / 2 x 10 9 = ...

20. A Needle in a Base-Stack: Cas9 Structural Biology

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    Blog Post

    ...not include stem loop 3, and guide RNA nucleotides 11-20 are not resolved due to structural disorder. HNH...DNA target are essential for cleavage. Nucleotides 11-20 are disordered and don’t appear in the Figure ... editing enzymes. Nature Reviews Microbiology, 20(11), 641–656. https://doi.org/10.1038/s41579-022-00739...