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We narrowed to 200 results for: ADAP

Showing: 21 - 40 of 200 results

  1. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    ...originally utilized CRISPR/Cas as a powerful and adaptive defense strategy against life-threatening viruses...scientists to hypothesize that prokaryotes developed an adaptive immune system that utilized various cas genes ... of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune system seems like the winning ticket to...

  2. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...structure. I quickly learned that my approach needed to adapt based on individual needs. For instance, I had one...projects autonomously. Failing to recognize and adapt to these differences could have led to frustration...experienced this firsthand when it took me some time to adapt to the communication style of a new, junior team...

  3. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    ...Executive Director changed twice. I had to learn how to adapt to different situations and, in particular, to different...predecessors. Changes were implemented and I had to adapt accordingly. For example, the quarterly and annual... would come necessary changes. I also learnt to adapt my leadership style according to the needs of my...

  4. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...method for genomic insertion. PITCh can also be adapted for whole-plasmid integration if you’d like to ...from a given promoter, and the technique can be adapted to other transgenes. It’s important to note that...increased versatility, it would be advantageous to adapt PITCh to insert genes into AAVS1, the “safe harbor...

  5. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    ...represent a functional CRISPR nuclease that could be adapted for genome editing. The use of a different nuclease... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...learn about CRISPR biology. Later work, like the adaptation of Cas13 to RNA targeting and RNA editing, has...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ...acids at the atomolar level. The Zhang lab has adapted natural RNase activity of the Cas13 protein to ...detection signal for a single target. SHERLOCKv2 was adapted so that a cleaved reporter could be detected on...built upon. Mammoth Biosciences and UCSF recently adapted the DETECTR platform to detect SARS-CoV-2 using...

  7. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ... of different basic BE technologies and their adaptation for plant genome editing. Cytosine base editors... at The Pennsylvania State University (PSU) is adapting and improving base editing platforms for plant...editing using hybrid prokaryotic and vertebrate adaptive immune systems." Science 353.6305 (2016): aaf8729...

  8. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    .../Cas9 for gene editing has expanded since its adaptation for use in mammalian cells in 2012-2013. Researchers... gene (although it has been quite successfully adapted for use in screening experiments as well). Therefore...fluorescence indicates a more successful gRNA design. Adapted from Mashiko et al., 2014. Not all gRNA’s are...

  9. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ... Figure 1: The Design-Build-Test-Learn cycle adapted for video making.   Cycle One: The Ironing ...on-camera presentations and interviews. But how can I adapt this setup for the lab, so we can get back to producing...shoot. I also added a teleprompter to the mix, adapting the setup to run some remote interviews, so the...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...populations. This kit consists of a 48 plasmid adapter library, with each plasmid carrying a unique barcoded...selective pressures: Miller, Corwin, et al. "Adaptation of Enterococcus faecalis to daptomycin reveals...lead to surprisingly large fitness effects during adaptive evolution of antibiotic resistance." Proceedings...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... of various G-protein-coupled receptors. Image adapted from Ino et al. 2022, under CC-BY license.   Find...activation by shift in cellular localization. Image adapted from Farahani et al. 2022, under CC-BY license....responses are highly resistant to pH changes. Image adapted from Koveal et al. 2022, under CC-BY license.   ...

  12. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...University of Wellington, Victoria, has developed an adaptable bacterial expression vector which gives close ...and C-terminal tags. Additionally, by using an adapted destination vector with a heterologous promoter...activation plasmids have great potential and have been adapted for use in mammalian cells, yeast, and Drosophila...during their transport. Finally, the system can be adapted to cellular screening to identify molecules that...a tool for monitoring multi-gene expression and adapt it for multi-color calcium sensing (plasmids coming...

  13. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...immune system. The techniques used here could be adapted to studying other systems in which there is large... revealing the cellular behaviors that mediate adaptive and innate immunity in diverse tissue environments... to better understand T cell initiation in the adaptive immune response. It was known that T cells were...

  14. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...Choose from different “Options”, including adding adapter sequences for cloning purposes.  Press run and... well as the suggested gRNA sequence including adapters. Output_UserInputParams: Contains all the information...subsequently cloned into your vector of choice using the adapter sequences that you have specified.   Run your...

  15. Deep Dive: qPCR

    Type
    Blog Post
    ...Table 1: Features of various qPCR chemistries. Adapted from Wong, 2018   Primers Design of primers...method is the Standard Curve method. See Table 2, (adapted from Wong, 2018) for a comparison of different ...comparison of different qPCR analysis methods. Table adapted from Wong, 2018.    🔥Hot Tip🔥 Negative...

  16. What's New in CRISPR - Winter 2018

    Type
    Blog Post
    ...Cas14 targets and cleaves ssDNA which they have adapted for detecting SNPs. Learn more from this Addgene...discovered in 2017. Now Dominik Niopek’s lab has adapted anti-CRISPR proteins for optogenetic control of...

  18. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibody-dependent cellular phagocytosis (ADCP). Figure adapted from van Erp, et al., 2019.    Antibody-dependent...Song, WC. Complement and its role in innate and adaptive immune responses. Cell Res 20, 34–50 (2010). https...

  19. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ..., the ability to embrace serendipity fosters adaptability, a crucial skill for a successful research career...research but also cultivate a mindset that is adaptable, creative, and poised for groundbreaking discoveries...

  20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...researcher. There existed, therefore, a need for an adaptable tool that could continuously monitor multiple ...- Overview of the neural and gut fiber system (adapted from Sahasrabudhe et al., 2023).   To develop...
Showing: 21 - 40 of 200 results