We narrowed to 84 results for: ALP

1. CASTing Off for New Shores in Human Genome Editing

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   ...s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...

2. SunTag and Fluorescent Imaging

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   ... al. modified the GCN4 sequence to increase its alpha-helical structure and stability, creating v4 SunTag...

3. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

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   ...right strain While common E. coli strains like DH5-alpha are recA-, some strains have been engineered to ...

4. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

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   ...neutralize many variants, including the B.1.1.7 (Alpha variant), B1.1.298, and B.1.429 (Epsilon), nearly...

5. Scientists Map the SARS-CoV-2-Human Interaction Network

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   ...selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...

6. Crowdfight, a Platform to Boost Scientific Collaboration During COVID-19 and Beyond

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   ...type of antibodies from an Andean mammal called alpaca could neutralize the SARS-CoV-2 virus. But while...

7. 5 Great Apps for Lab Life

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   ...restriction site to a primer, you only need to skim the alphabet on the side of the screen to find the sequence...

8. Targeted Mutagenesis with EvolvR

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   ...PMID: 30253789. PubMed Central PMCID: PMC6154929. Halperin, Shakked O., et al. "CRISPR-guided DNA polymerases...

9. Viral Vectors 101: The AAV Retrograde Serotype

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   ...retrograde tracer) (Fig. 2). Viruses as neuronal scalpels Viruses can be injected into the brain and efficiently...

10. Plasmids 101: Methylation and Restriction Enzymes

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    ...cleave DNA. Common lab E. coli K12 strains such as DH5alpha contain 3 methylases that recognize and methylate...

11. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

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    ... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...

12. Plasmids 101: Yeast Vectors

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    ... LYS2 L-lysine yes - Grow on plates containing alpha-aminoadipate in the absence of a nitrogen source...

13. Plasmid Tools for Microbiome Studies

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    ... plasmids could also be transformed into Alphaproteobacteira, Betaproteobacteria, and Gammaproteobacteria...

14. Gendered Innovations: Why Does Sex of the Cell Matter?

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    ...another excellent resource for research design is Kalpit Shah et al., “Do you know the sex of your cell?...

15. Mouse Modeling, Part 1: Genetically Engineered Mice

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    ... the ligand binding domain of estrogen receptor alpha (Esr1). Mice are fed or injected with tamoxifen,...

16. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ... high-throughput screen for molecules affecting alpha-synuclein oligomerisation. Eastwood T, Baker K, ...

17. Hot Plasmids February 2024

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    ...versions. Thanks to Christophe Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this...

18. Viral Vectors 101: Virus Safety

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    ...Virus Many virus experiments involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment...

19. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...Sickle Cells Disease, Duchenne Muscular Dystrophy, Alpha1-antitrypsin deficiency (most widely used so far...

20. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...flies before, get yourself a copy of Fly Pushing by Ralph J. Greenspan. It’s a fantastic book for newbies ...