We narrowed to 569 results for: BLI;

...proceeding in 99% of 12-fragment assemblies and over 90% for 24-fragment assemblies (Figure 3, Table 1). These...for Golden Gate Assemblies with BsaI-HFv2 and T4 DNA ligase. Efficiency of assemblies per plate using ...reaction to form complex, multi-insert modular assemblies that enable biosynthetic pathway engineering ...optimization. However, current best practices for assemblies of more than 10 modules often rely on two-step...placed practical limits on single- or two-step assemblies. We have reduced these limitations through the...that it is now possible to achieve 20+ fragment assemblies with both robust efficiency and accuracy. Golden...cassette. Additional confirmation of accurate assemblies was achieved by the sequencing of plasmids isolated...

...the OpenWorm website. microPublication microPublication is a unique publication for the worm community...resource for scientists to publish findings that don't fit into larger publications and would otherwise be... referenced. In addition the data published in microPublications is curated and added to other databases...introducing point mutations that was published in a microPublication (Schwartz and Jorgensen, 2018).  These... Map & Network (CeNGEN) The CeNGEN goal is to establish a comprehensive gene expression atlas of every...aims and approach of CeNGEN, check out their publication.   3’UTRome The 3’UTRs of mRNA are important ...community where one can publish brief novel findings. These results are normally one figure and can be negative...

...shared by the authors on a public server independent from peer-reviewed publishing in a journal.  Accelerating... with each other: from formal mechanisms like publishing a research article, presenting a conference poster...while peer review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...increasingly growing time from submission to publication. It takes many months, often more than a year,...avoid many of these issues, by sharing their work publicly and immediately once the manuscript is drafted...and updated by the authors over time, providing a public record of changes, critiques or endorsements. In...work is still in progress or awaiting a final publication in a journal.  Preprints can be one tool in a...

...of the largest bibliographic databases available, Web of Science. For each publication, Web of Science...citations in its first four years (1). In addition to publishing in Science, Professor Zhang deposited the associated...Science provides basic bibliographic information and uses its vast network of articles to track the total number...Science, PLOS One, and PNAS. We then broke the publications from these journals into two groups: those containing...CRISPR/Cas papers and Addgene deposits, but papers published in other years also get many more citations This...For example, it is very plausible that science published in more widely-read journals gets more citations...adjust our estimates based on the journal that published the articles.  That is, we re-estimate the citations...

...to inform the public (8–13). Facing the possibility that reason is losing ground in public discourse, many...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-... Idea Pew Research Center: Public Praises Science; Scientists Fault Public, Media March for Science: Principles...autism, and other hypotheses mount yet policies and public opinion do not seem to take note. For example, ...consumption in comparison to 37% of the general public (5, 6). Similarly 90% of active research scientists...to human activity while much less of the general public agrees (5, 6). Many of the new administration’s...Lost trust Perhaps the greatest risk is that the public will lose trust in scientists if the march highlights...

...favorite gene was described in a CRISPR screen publication. You see this mentioned in the results section...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to...manually curated and annotated from more than 45 publications, beginning with the seminal research articles... makes it possible for researchers to query all public CRISPR results for any gene of interest. In addition...screen curated in ORCS is linked to the original publication via its PubMed identifier, which can also be ...screens of interest.   Figure 3: Each publication featured in BioGRID ORCS has a dedicated summary...

...research project, the vast majority of what gets published in scientific journals flies under the radar and...be part of the answer. Problems with written publications Online tools like ResearchGate, GoogleScholar...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share...

...not currently publish career outcomes for PhDs or postdocs. Having this information publicly available may... career development. Publishing Panel The Early Career Researchers in Publishing Panel brought in editors...variety of publications as well as young PIs and all gave advice on how to succeed in publishing in the current...university publication, improving your general writing skills will help you in your academic publishing goals...Path Panel and the Early Career Researchers in Publishing panel, focused on showing researchers that they...cognizant of this during my PhD. Submitting a publication doesn’t have to be like throwing it into a black...have any questions or concerns throughout the publishing process. The diversity panel - Equity and inclusion...

...Organizing a science conference for the public How does one organize a public scientific conference? As a member...DayCon is a one-day conference aimed at the general public that provides an accessible introduction to various...the communication gap between scientists and the public and help make science more transparent. Second,... their communication skills and engage with the public. The organization runs a wide variety of programs...conference-style experience that was accessible to the general public both in terms of cost and content level. Sponsorship...science outreach can be, both for scientists and the public. For students, these opportunities to improve our... alternative careers in science. In return, the public receives trustworthy, intelligible scientific resources...

...you are trying to clone. Figure 2: MoClo assemblies depend on compatibility of four-base overhangs...tools When available, always perform your MoClo assemblies in silico before you start doing your cloning...sequentially for your Level 0, Level 1, and Level 2 assemblies using the plasmid sequence files provided with...synthesized DNA fragment can be used directly in your assemblies. Preparing reagents Keep it cool You’ve done ...kit plate for fresh bacteria. Performing your Assemblies Calculate your volumes As you progress through...high throughput You are ready to perform your assemblies, but you have a high number to get through. It... It’s time to think big and perform your assemblies in a high-throughput format. If your lab owns a liquid...

...to be doing in humans; We have not had a broad, public conversation or consensus around the ethics of ...being experimented on, too.) However, as research published on December 13 shows us, CRISPR/Cas9 techniques... us with the second critique. Absent of a broad public consensus on genome editing, there was a generally...revisited at meetings when sufficient time, additional public fora for data review, and more lab research could...could be used to bring scientists and the public together to build consensus guidelines, but there was ...t have to look far for proof that this model of public involvement, research, and consensus can happen...Council. While the Nuffield Council has recently published a report on the social and ethical issues of genome...

...number of organizations in public engagement efforts, including giving public lectures, writing science...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...between these communities and the biomedical establishment. This, along with concerns about the use of ...coursework and attending seminars in bioethics, public health, science policy, and science and technology...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...organizations (often volunteer-based) that put on public lectures, science cafés or science festivals, and...for science-outreach organizations or student publications, submitting op-eds to your local newspapers,...

... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts... focus on getting this information out into the public where it can be utilized by others. Addgene’s AAV...citable DOIs through DataCite before being made publicly available through the Data Hub. These minimum ...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and...

...science to the public, talking to policy-makers, and broadly defending science in the public sphere as often...Outside the lab, she dabbles in science policy, public outreach, and restorative justice.  Follow her ...devaluing science has alarming implications for our public policies—many of which impact reproductive rights...forgetting how to engage with friends, family, and the public about its importance.  I think many of us grew ... proposed.  It's our responsibility to join the public conversation and to tell the stories behind our...legislators and because the march had been so well-publicized, we might encounter hostile anti-science activists...scientist. I’ve worked tirelessly to get the publications, grant funding, and teaching experience that...

...pre-clinical life science work, economics) that published research is difficult to replicate.  Why is replicating...appreciate how few details are provided in most published papers. The precise timing, order, or every combination... rarely exist or are preserved as part of the publishing process and become lost if not archived. Remove...process of creating a time-stamped research plan in a public repository before conducting the experiment (note...registries do require that research plans become public eventually). It is typically done right before ... article is given “in-principle acceptance” to publish the final results regardless of the outcome of ...null findings that are notoriously difficult to publish.  With Registered Reports, the authors are able...

...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...doi.org/10.1038/nbt.3199 Find plasmids from this publication at Addgene. Klann TS, Black JB, Chellappan M...doi.org/10.1038/nbt.3853 Find plasmids from this publication at Addgene.  Lei Y, Zhang X, Su J, Jeong M, ...10.1038/ncomms16026 Find plasmids from this publication at Addgene. Liu XS, Wu H, Ji X, Stelzer Y, Wu.../j.cell.2016.08.056 Find plasmids from this publication at Addgene. Maeder ML, Angstman JF, Richardson...doi.org/10.1038/nbt.2726 Find plasmids from this publication at Addgene. McDonald JI, Celik H, Rois LE, Fishberger.../10.1242/bio.019067 Find plasmids from this publication at Addgene. Mendenhall EM, Williamson KE, Reyon...

...lab while the publication page contains all the plasmids deposited from the same publication. Next up, we... available through Addgene. Publication: If the plasmid has been published, the paper in which it was ...through the depositing lab page, then onto the publication page, and finally on down to the individual plasmid...Great! Now, if I want to use this plasmid in a publication, how do I know who to acknowledge? Depositing...the bottom of the page that help you cite this publication in your own work: How to cite this plasmid: The...depositing lab whenever their plasmids are used in a publication. Addgene makes this easy by providing a template...of our plasmid pages, and provides a list of publications which have used the plasmid (see our blog post...

...to peer-reviewed journal publications, although many don’t necessarily publish in OA journals themselves...in annual R&D expenditures to support increased public access to the results of federally-funded research...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote...an organization called Free the Data launched a public database to gather the results of Myriad's genetic... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which...submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to press, no less...gave preference to journal access granted via a public repository or authors’ own web sites. 9)      The...

...scientific publishing world and debate issues about how to improve the scientific publishing process. There...variety of careers and jobs. At Science, which is published by the American Association for the Advancement...as financially critical. Those who work on the publishing side of science have professional meetings, societies...access affects financial models in information publication. Take a look at the blog ‘Scholarly Kitchen’ ...’ for a glimpse into the world of scholarly publishing. And see for example the Eighth International Congress... Congress on Peer Review and Scientific Publication, where diverse approaches to peer review were discussed...Hines is a Senior Editor at Science magazine, published by the American Association for the Advancement...

...Co-Ordinating Centre for Public Engagement (NCCPE) provides on-site courses in public engagement for institutions...communication activities, e.g. tweet it when you publish a new blog post, and live tweet from conferences...tips on using social media as a scientist here. 2) Public engagement - Participate in science festivals and... and outreach If you are comfortable talking in public, volunteer at science festivals. Or engage with...performance. When your institute opens its doors to the public, for example at an anniversary or for Girls’ Day...for 10 busy weeks at media outlets like National Public Radio, WIRED, Scientific American or National Geographic...projects Lastly, if you'd like to directly involve the public in your research projects, you can set up a citizen...