We narrowed to 94 results for: Braf;
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TypeBlog Post...used FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells..., a time consistent with previous methods. In zebrafish, the Shu Lab used FlipGFP to track the spatiotemporal...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...developed the LipoGlo system in zebrafish (Thierer et al, 2019). Zebrafish are a great model for high throughput... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...CreLite for cell tracing using the transgenic zebrafish model, ubi:zebrabow. In this system, cells express...found in both deep and superficial tissues of zebrafish studied, such as the brain, eye, muscle and epithelial... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog Post...other species such as non-human primates [7, 8], zebrafish [9], and c. elegans [10]. If used properly, optogenetics...Wyart. "Optogenetics: a new enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3... -
27 Hot Plasmids from 2016
TypeBlog Post...cloning steps. The FusX system was verified in zebrafish and was shown to offer high activity and unparalleled...Magneto2.0 was also tested in vivo by expression in zebrafish and mice. These in vivo studies revealed that ...pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA Zebrafish Expression Human kinase domain constructs... -
Our Most Popular Blog Posts from 2019
TypeBlog Post...five model organisms: mouse, fruit fly, yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox... -
What's New in CRISPR - September 2019
TypeBlog Post...predicted to target sites in the human, pig, or zebrafish genome, this system can be used in these organisms... -
Chemogenetics vs. Optogenetics: Which Method Should I Choose?
TypeBlog Post...organisms with light accessible brains, such as zebrafish larvae. Mouse studies, however, require a permanent... -
Some Like it Hot: Thermostable GeoCas9
TypeBlog Post...mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not thermophilic (high... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog Post... to successfully track nuclei in a developing zebrafish embryo over an 80 minute time course. By stitching... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...be used in mouse, Drosophila, C. elegans, and zebrafish – essentially all of the standard organisms for... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation iGluSnFR: SF-iGluSnFR... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog Post...of animal models such as mice, C.elegans, and Zebrafish. Find Archon1 plasmids at Addgene! Piatkevich ... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, Davidsohn N, Housden BE, Perrimon N, Weiss R... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post... taurus (bovine), X. laevis (frog), D. rerio (zebrafish), D. melanogaster (fly), C. elegans (nematode)... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...studies, though other organisms such as rats or zebrafish are also used. With the development of viral vectors... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...the worm community but there are Drosophila and zebrafish articles published there as well. Worm Development... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...available for neuron-specific expression in both zebrafish and mammalian systems. The GCaMP6, jGCaMP7, and... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...developmental models including flies, nematodes, and zebrafish Find plasmids for Mammalian RNAi ... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog Post.... You can also use the library of full-length zebrafish rab proteins from Rob Parton's Lab (University...