We narrowed to 94 results for: CAG
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TypeBlog Post...Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral vector...
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Viral Vectors 101: Calcium Sensors
TypeBlog Post...hSyn promoter) and pan-mammalian cell expression (CAG promoter). An improved version named CaMPARI2 is ... -
Optogenetics AAV Preps
TypeCollection...28017 AAV-CAG-hChR2-H134R-tdTomato CAG ChR2/H134R tdTomato Constitutive rg* Svoboda 75470 pAAV-CAG-FLEXFRT-ChR2... pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG Jaws GFP Cre dependent 1, 5, rg* Boyden 84446 pAAV-CAG-FLEX-rc...Constitutive 1, 2, 5, 9 Deisseroth 127090 pAAV-CAG-DIO-ChR2(H134R)-eYFP CAG ChR2/H134R EYFP Cre dependent PHPeB ...Constitutive 8 Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent...Cre dependent 9 Harvey 108912 pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 Cre...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato... dependent 5 Yizhar 109048 pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST CAG eGtACR1 (soma-targeted) mRuby3 ... -
Brain Initiative Collection
TypeCollection...fluorescent protein EYFP from the CAG promoter Viviana Gradinaru 104055-AAV2 pAAV-CAG-eYFP An AAV genome that ubiquitously...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAV5 pAAV-CAG-eYFP An AAV genome that ubiquitously...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-AAVrg pAAV-CAG-eYFP An AAV genome that ...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104055-PHPeB pAAV-CAG-eYFP An AAV genome that ...fluorescent protein eYFP from the CAG promoter Viviana Gradinaru 104061-PHPeB CAG-NLS-GFP AAV expression of nuclear...AAV1 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter...AAV5 pAAV-CAG-dLight1.1 To generate Adeno-Associated viruses for expression of dLight1.1 under CAG promoter... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog Post... Find the Cre-expressing viral preps here AAV-CAG-dLight1.3b from the Lin Tian Lab is now available... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post...assemble, including GC rich regions. For example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer... -
Brain Armamentarium
TypeCollection... M. Wilson 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana...Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the CAG promoter Edward Boyden Viviana... -
New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...promoters, including ubiquitous ones such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post... version is commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter... -
AAV Molecular Tools
TypeCollection...Expression System Activity Serotype PI 99118 pAAV-CAG-tTA CAG-driven, constitutive Expression of the tet-off...separate) tdTomato 8 Scanziani 34910 paavCAG-pre-mGRASP-mCerulean CAG-driven, constitutive Expression of... connectivity mapping 5 Kim 34912 paavCAG-post-mGRASP-2A-dTomato CAG-driven, constitutive Expression of... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...overlapping 5′ and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment...cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA plasmid (Mashiko et al. 2013). The pCAG-EGxxFP target...cloned directionally into the BbsI site. (c) The pCAG-EGxxFP target plasmid was co-transfected with pX330...Once amplified, you can insert this region into a pCAG-EGXXFP plasmid using standard cloning techniques...of transfecting HEK293T cells with your modified pCAG-EGXXFP plasmid along with the individual gRNAs in...Remember the primers you designed to generate your pCAG-EGXXFP plasmid? They are the perfect primer sets... -
The Pleiades Promoter Project
TypeCollection...Positive Control CAG promoter pEMS1293 cre N/A CAG promoter pEMS1164 EGFP/cre N/A CAG promoter pEMS1114...EGFP/cre/NLS N/A CAG promoter pEMS1157 EGFP/NLS N/A CAG promoter pEMS1277 EGFP/NLS N/A CAG promoter pEMS1294...pEMS1294 intron-lacZ/NLS N/A CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson... -
Luciferase Plasmid Collection
TypeCollection...83282 pAAV-CAG-RLuc Renilla CAG AAV expression of Renilla luciferase Mark Kay 83280 pscAAV-CAG-RLuc Renilla...luciferase expression Stephen Tapscott 83281 pAAV-CAG-FLuc Firefly CAG AAV expression of firefly luciferase Mark...Renilla CAG Vector for packaging self-complementary AAV expressing Renilla luciferase Mark Kay 140328 pLenti-PGK-Venus-Fluc... -
Serotype Testing AAV
TypeCollection...example, AAV1). AAV Vectors for Serotype Testing pAAV-CAG-GFP (Plasmid #37825) Description : Ready-to-use AAV...Edward Boyden ) and direct GFP expression from the CAG promoter. For information about each catalog item... -
Recombinases AAV Preps
TypeCollection...pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible Recombinases... -
TALEN Plasmids and Kits
TypeCollection...pC-GoldyTALEN directs expression of TALENs from a truncated CAGs promoter. RCIscript-GoldyTALEN is designed for in... cells. TALEN expression is driven by the strong CAG promoter or can be achieved by in vitro mRNA synthesis...fragment for blue/white-screening in E.coli , (iii) CAG promoter and Kozak sequence to drive efficient TALEN...Golden Gate TALEN 2.0 37184 pCAG-T7-TALEN(Sangamo)-Destination Pawel Pelczar pCAG-T7-TALEN(Sangamo)-Destination...mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination 40132 pCAG-T7-TALEN(Sangamo)-FokI-...robust cleavage activity in several later studies. pCAG-T7-TALEN(Sangamo)-Destination vectors are available...assembly. Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog Post...different cells. Promoters such as chicken-beta-actin or CAG can drive strong early gene expression, but can become... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...thoroughly tested in your cells. Some promoters, such as CAG are very strong and may require less viral particles... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post...integration is TA dinucleotides and it leaves behind the CAG DNA footprint from its terminal sequences at the ... -
Chemogenetics AAV Preps
TypeCollection...GlyR Promoter Synapsin CaMKIIa CD68 Dlx GFAP nEF CAG E2 regulatory element Tag Fusion tags mCherry HA ...mCherry fusion none 1, 2, 5, 8, 9, rg* Roth 52536 rAAV-CAG::FLEX-rev:: hM4D-2a-GFP hM4D(Gi) - Inhibition GFP...