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We narrowed to 46 results for: Dre recombinase

Showing: 21 - 40 of 46 results

  1. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    ...Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow...143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide...Drosophila melanogaster. Hadjieconomou D, Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods...

  3. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5... Email us or check our website to access them! DREADDs Plasmid Serotype Name  44361  AAV1 pAAV-hSyn-DIO-hM3D...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...open-reading frame (DIO). Upon coexpression with cre recombinase (either from a mouse line or viral co-injection...specificity dependent on the promoter of the cre recombinase while still taking advantage of the robust expression...things in science, optogenetics is best used to address a specific question. Optogenetics is a tool and...

  5. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...once injected, the DREADD will only be expressed in cells that express cre recombinase. Typically, this ...consists almost entirely of viruses expressing DREADDs from Bryan Roth’s lab at the University of North...floxed human muscarinic receptor 4 (Gαi-coupled) DREADD fused to mCherry, all under the control of the ...further specifies neuron-specific expression of the DREADD (Kügler et al., 2003), which can then be exclusively...mice, rats, or non-human primates. To activate the DREADD, CNO is delivered either through drinking water...

  6. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ... of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce modifications...very old branch of science, dating back several hundred years, with historical ties to psychology and philosophy...an introduction to behavioral neuroscience, and address experimental design considerations used in all ...enough to target larger areas, typically a few hundred nl. Alternatively, for larger brain areas, repeated...

  7. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...strategies that combine prime editing with a recombinase to install large sequences (up to 10 kb) into... in mammalian cells via continuously evolved recombinases and prime editing. Nature Biomedical Engineering...This recent contribution by the Adamson lab also addresses the problem of RNA stability. Using a CRISPRi ...

  8. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...AAV serotypes, and the ever-growing variety of recombinase systems. Picking Ligands Picking the right ligand...used in chemogenetics are DREADDs and PSAMs. Using DREADDs DREADDs DREADDS, or designer receptors exclusively... Other DREADDs, such as the KORD receptor, increase this threshold and silence neurons. DREADD-activated...function (Saloman, JL, et al., 2016).   DREADD ligands Several DREADDs, including hM3D(Gq), have been derived...cellular responses. The cellular response set off by a DREADD depends on what G-protein and, consequently, what...what pathway the receptor acts through. The DREADD hM3D(Gq), for example, lowers the threshold for neuronal...popular agonist for muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns...

  10. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ... expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral vectors...vectors. Both inhibitory and excitatory DREADDS from the Gether lab are now available as ready-to-use AAV vectors...

  11. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...its expression. Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing...expression in the central nervous system (CNS). To address this technological gap, a trio of recent papers...isoform-specific expression of Bdnf resulted in hundreds of differentially expressed genes between Bdnf...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ... (Howerton at al. 2014). Addgene has several recombinase-expressing ready-made AAV vectors which can be...Hypothalamic CNTF volume transmission shapes cortical noradrenergic excitability upon acute stress." The EMBO journal.... Bahi, Amine, Vijay Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of ...

  13. Worm Expression Resources

    Type
    Collection
    .... elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific...experiments. The C. elegans genome is well studied and hundreds of reporters have been developed and characterized... General Tools Fire Lab C. elegans Vector Kit - Andrew Fire Lab. A set of vectors for C. elegans research...fusion vectors. C. elegans optimized fluorophores - Andrew Fire Lab. Available with and without PATC, which...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...Addgene New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also ...hDlx-FLEX-GFP, hDlx-Flex-dTomato, mDlx-GCaMP6f, hDlx-GiDREADD-dTomato and mDlx-ChR2-mCherry. Find these AAVs...

  15. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...sequence of interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription...off-target effects, a distinct contrast to the hundreds of observed shRNA off-targets. They subsequently...

  16. Bacterial Expression Systems

    Type
    Collection
    ... Kumar 134405 pBbAW4k-loxP-TT-loxP-mRFP1 Cre recombinase activity Fluorescence (mRFP1) Escherichia coli...Collection for a selection of plasmids containing recombinases to induce targeted genetic inversion, deletions...pDawn FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 68940 pRMC2 Pxyl/TetO Anhydrotetracycline... pR_FixK2 Blue light (470 nm) Escherichia coli Andreas Moeglich 26092 pET15-MHL T7-lacO Lactose/IPTG Escherichia...

  18. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP, allowing...all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow cassette...signaling. For more information on DREADDs, please see the Roth lab's DREADD users blog. New CRISPR plasmids...alleles off or on enables this method to rapidly address questions of tissue-specificity and cell autonomy...bacteria are time-consuming and involve many steps. The Drew Endy and Keith Shearwin labs have developed a new...al., ACS Synth Biol 2013 Sep 20;2(9):537-41. DREADD-based chemogenetic technologies After several...Receptors Exclusively Activated by Designer Drugs (DREADD) plasmids on his own, UNC-Chapel Hill's Bryan Roth...

  19. CRISPR Guide

    Type
    Collection
    ...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...mechanisms, including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon... large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor...lab , pairs prime editing with a large serine recombinase (LSR). A prime editor is used to install the ...evoBxb1 or eeBxb1 are evolved versions of the recombinase with improved integration efficiencies in mammalian...& Hsu, P. D. (2022). Systematic discovery of recombinases for efficient integration of large DNA sequences...locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites throughout the chromosome...

  20. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...specialized system with parts including site-specific recombinases, regulatory elements, developmental regulators... constructs (Martella et al., 2017). If you can dream up an experiment, MoClo can help you achieve it!...://doi.org/10.1101/2024.02.16.580396. Martella, Andrea, et al. “EMMA: An Extensible Mammalian Modular ...
Showing: 21 - 40 of 46 results