We narrowed to 36 results for: Flp recombinase
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TypeCollection...virus, plasmids expressing both the Cre and Flp recombinases to remove genes flanked by either loxP or ...variety of reporters as well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors...EF1 promoter when FLP is expressed by another vector pCDH-EF1-FLPe 72262 Expresses FLPe from the EF-1 promoter...72260 Expresses copGFP from the EF1 promoter when FLP is expressed by another vector pCDH-EF1-Fon-tdTomato...from the CB promoter pCDH-CB-FLPe-P2A-copGFP-T2A-Puro 72258 Expresses FLPe, copGFP and the puromycin resistance...resistance from the CB promoter pCDH-CB-FLPe-P2A-tdTomato 72259 Expresses FLPe and tdTomato from the CB promoter...tdTomato from the CMV promoter pAdxEF1-FLPe-tdTomato 73352 Expresses FLPe and tdTomato from the EF1 promoter...
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Recombinases AAV Preps
TypeCollection...Packaging Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...rg* Zhang 75469 pAAV-EF1a-Flp-DOG-NW EF1a none 1 Cepko 183412 pAAV-CAG-FlpO CAG none 1, rg* Janelia Light-Inducible...collection of site specific recombinases. Viral... Viral Vector Packaging Service AAV Recombinases Viral Vector Packaging...in-stock AAV preps that encode site-specific recombinases, which can be used to control gene expression...Light-Inducible Recombinases ID Name Promoter Fluorophore Serotype(s) PI 140135 pAAV-EF1a-iCreV EF1a none...5, rg* Zeng Flpo AAV ID Name Promoter Fluorophore Serotype(s) PI 51669 AAV phSyn1(S)-FlpO-bGHpA Syn none... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog Post... is achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...fluorescent proteins (EGFP, mCherry etc.), recombinases (Cre, Flp etc.), and tetracycline drug induction ...marker, likely by breeding to a mouse line with a recombinase. If it turned out your chimeric mice did not ...ssDNA. Here, we’ll use the example of inserting a 2A-FlpO cassette into the Fgf8 gene. Miura et al. (2018)... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post.... We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral... -
Worm Expression Resources
TypeCollection... C. elegans by combining expression of the FLP recombinase from either a heat-shock promoter or a tissue-specific...diminishes epigenetic silencing in the germline. A "FLP-Out" system for controlled gene expression in Caenorhabditis...tissue-specific promoter and expression of the target FLP-out cassette from either a ubiquitous or a tissue-specific... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...The old standby of CRE/flp didn’t work – it turns out these tyrosine recombinases are as bad at integrating... -
New Viral Vectors - Winter 2025
TypeBlog Post...pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV2 Recombinase James M. Wilson New serotype pENN.AAV.hSyn.Cre.WPRE.hGH AAV2 Recombinase James M...AAVrg Recombinase James M. Wilson New serotype pENN.AAV.CamKII 0.4.Cre.SV40 AAVrg Recombinase James ...Optogenetics Ofer Yizhar New serotype pAAV-syn-Flpo AAVrg Recombinase Ian Wickersham New viral prep pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40...-iCre(R297T)-BGHpA (Alias: CN4825) AAV-PHPeB Recombinase Jonathan Ting New viral prep AiP15140: pAAV-AiE0873m... -
AAV Viral Preps
TypeCollection...Channelrhodopsins, Halorhodopsins, Archaerhodopsins Recombinases Cre, Flp, Dre Biosensors Calcium Sensors, Glutamate... -
CRISPR Plasmids - Tagging
TypeCollection.... An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out. Plasmids...assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general cloning plasmid, and... -
Brain Initiative Collection
TypeCollection...pAAV-CAG-fDIO-mNeonGreen An AAV genome with Flp recombinase-dependent expression of mNeonGreen from the...light-inducible site-specific iFlpV recombinase Hongkui Zeng 140137-PHPeB pAAV-EF1a-iFlpV can be used to generate...expresslight-inducible site-specific iDreV recombinase Hongkui Zeng 140137-AAV1 pAAV-EF1a-iFlpV can be used to generate ...will express light-inducible site-specific iFlpV recombinase Hongkui Zeng 140552-AAV9 pAAV-hsyn-GRAB_5-...83894-AAV1 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV2 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic...83894-AAV5 pAAV-hDlx-Flex-dTomato-Fishell_7 Cre recombinase-dependent dTomato expression in forebrain GABA-ergic... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...developed three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP,...all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow cassette... -
Adenovirus Guide
TypeGuide...have been developed, such as using the Cre-lox and Flp-FRT recombination systems to excise the packaging... production, and is driven by use of the Cre recombinase. Genome Integration Upon infection, wild-type... -
Caltech Systemic Capsids
TypeCollection...light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase iDreV Zeng ...Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre..._ChR2_mCherry BiSSTe4 Activator ChR2 Fishell Recombinases 105540 pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 Syn... -
Hot Plasmids - August 2020
TypeBlog Post...New serotypes for the Cre-dependent Flpo recombinase pEF1a-DIO-FLPo-WPRE-hGHpA, which is now also available... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...ID Plasmid Allen Institute ID Enhancer ID FP/Recombinase Labeled Population Region 230551 pAAV-AiE2638m-minBG-SYFP2...Isocortex 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Layer 6_IT_Car3 Isocortex 224000...Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex...Isocortex 230607 pAAV-AiE2103m-minBG-FlpO-WPRE-HGHpA AiP1252 AiE2103m FlpO Lamp5 interneurons Isocortex 223971...subplate 230518 pAAV-AiE2051m-minBG-FlpO-WPRE-HGHpA AiP1211 AiE2051m FlpO Claustrum Cortical subplate 191720...214855 pAAV-AiE0779m_3xC2-minBG-FlpO-WPRE3-BGHpA AiP14201 AiE0779m_3xC2 FlpO DRD1 Medium spiny neurons (D1...Striatum 230871 pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA AiP14573 AiE0452h FlpO DRD2 Medium spiny neurons (D2-MSN...