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Showing: 21 - 40 of 49 results

  1. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Optogenetics Karl Deisseroth New serotype AAV-CamKIIa-jGCaMP8s-WPRE AAV5 Biosensors Loren Looger New serotype...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...and the canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur...

  11. Five Popular Model Organisms

    Type
    Blog Post
    ...expresses a genetically encoded calcium sensor, GCaMP6 (Nguyen et al., 2015). If you are interested in...

  12. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... 5’ CCGG AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT TTTTTG 3’ Reverse oligo: 5’...AATTCAAAAA AATGCCTACGTTAAGCTATAC CTCGAG GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO...

  13. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...adam15 TAL3016 & TAL3017 TGTTTCTGGCGGACTCTCgtcaccattcttctgtTTGAAGGCACACCTTTGA ahcyl1 TAL3018 & TAL3019...TAL3019 TGACAGACTGTGGCGAGGatgccaagctggaggcGACGAAACAGGCCAGCAA amh TAL3210 & TAL3211 TCCAGGCAAGATTTGGGCtgatgctgatgatgacCGTGGCGATTGGGTCGTA...TGATCTGGATCCTAACAAggatgacttgcccctgtGTGCGAACACCAACCATA arf6 TAL3212 & TAL3213 TGGGGAAGATGCTTTCCAagatcttcggcaacaaGGAGATGAGAATATTGA arnt1 TAL3214 & TAL3215 TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA... TCCCGTCTCTGGCCATGAcctcctctaatggatcTCATTCCAACGGTGTGCA apoa4 TAL3022 & TAL3025 TGGCAGGACCAACCAATGcccagcatggacctggTGAAAAATGCTTTCTGGA...arap3a TAL3026 & TAL3027 TGGCAACAATCGGTCCGTgctcagatgcagaggaCCTGTTGGCTTCCATCCA ARSA TAL 3002 & TAL 3003... asun TAL3404 & TAL3405 TAACTCAAGCCAACTCCAggaaacatcaggagtttGCAGGAAGATTGAGTTCA atf6 TAL3406 & TAL3407 TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA... TCCAACCAGCAAAAAGACccatccaggtgtgccccaAGGTTTCCATTCAGCCTA atg5 TAL3218 & TAL3219 TAAAGTCAAGAAACATTTtctcaaagtcatgaagGCAGAGGATGTGGAGGAA...

  14. Biosensor AAV Preps

    Type
    Collection
    ...Calcium Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch2B Yellow...Constitutive 9 Li Calcium Sensor: GCaMP8f 162376 pGP-AAV-syn-jGCaMP8f-WPRE Syn jGCaMP8f none Constitutive 1, 9, ...pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent 1, 5, 9, rg* GENIE 169258 AAV-hSyn-Soma-jGCaMP8f Syn...soma-jGCaMP8f none Constitutive 1, 9, rg* Fyhn 176750 AAV-CamKIIa-jGCaMP8f-WPRE CamKIIa jGCaMP8f none ...AAV-mDlx-jGCaMP8f-WPRE Dlx jGCaMP8f none Constitutive 1, 9, rg* Looger 176756 AAV-EF1a-jGCaMP8f-WPRE EF1a...AAV-CAG-jGCaMP8f-WPRE CAG jGCaMP8f none Constitutive 1, 9, rg* Looger Calcium Sensor: GCaMP8s 162374 pGP-AAV-syn-jGCaMP8s-WPRE...-jGCaMP8s-WPRE Syn jGCaMP8s none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG...

  15. Validated gRNA Sequences

    Type
    Collection
    ...victoria GGGCACGGGCAGCTTGCCGG 46760 cut S. pyogenes 23918387 Chen EGFP A. victoria GGGCACGGGCAGCTTGCCGG 47511...sapiens GCCCAGCCTCCGCACCTACA 61833 cut S. pyogenes 24424410 Ye gsk3b D. rerio GGGACCTGACCGGCCGCAGG 42245...sapiens GATACTGGAGAAGCAGGGGC 68466 cut S. pyogenes 26145478 Zhang PAX6 H. sapiens GTAGATTTTGTATGCACTGC 68465... PcfiA AAACAAAACCTCATCAGGCA 68900 interfere S. pyogenes 26918244 Lu PcfiA GAAGCTCACTCCTTAGCACG 68899 interfere...thaliana CACTAGGAGCAACACCCAA cut S. pyogenes 26479191 Kim P450 O. sativa CATATAGTTGGGTCATGGCA cut S. pyogenes...musculus ACAGGCATATGGTTGTCCAT 79005 cut S. pyogenes 26944679 Shaw Tfeb M. musculus AGCACTGTTGCCGGCCGAGG 79006...Species PubMed ID Depositor OCT4 H. sapiens CTCCCATGCATTCAAACTG 66989 cut S. pyogenes 26028531 Huangfu OCT4...

  16. Retrograde AAV viral preps

    Type
    Collection
    ...Dre Calcium Sensors GCaMP8f GCaMP8s GCaMP8m GCaMP7f GCaMP7s GCaMP7b GCaMP6f GCaMP6s GECO Other Biosensors...pGP-AAV-syn-jGCaMP7s-WPRE Syn GCaMP7s Calcium sensor Kim 104489 pGP-AAV-syn-jGCaMP7b-WPRE Syn GCaMP7b Calcium...-syn-jGCaMP8s-WPRE Syn GCaMP8s Calcium sensor GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn GCaMP8m Calcium...AAV-hSyn-Soma-jGCaMP8m Syn Soma-GCaMP8m Calcium sensor Fyhn 169258 AAV-hSyn-Soma-jGCaMP8f Syn Soma-GCaMP8f Calcium...-mDlx-jGCaMP8s-WPRE Dlx GCaMP8s Calcium sensor Looger 176756 AAV-EF1a-jGCaMP8f-WPRE Ef1a GCaMP8f Calcium...AAV-EF1a-jGCaMP8m-WPRE EF1a GCaMP8m Calcium sensor Looger 176758 AAV-EF1a-jGCaMP8s-WPRE EF1a GCaMP8s Calcium...AAV-CAG-jGCaMP8f-WPRE EF1a GCaMP8f Calcium sensor Looger 179255 AAV-CAG-jGCaMP8m-WPRE CAG GCaMP8m Calcium...

  17. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...gfaABC1D-lck-GCaMP6f Biosensor Baljit Khakh AV-5-PV3435 100834-AAV5 pENN.AAV.CamKII.GCaMP6f.WPRE.SV40 Biosensor...AV-1-50942 50942-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...AV-1-50943 50943-AAV1 pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68717 68717-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...-68719 68719-AAV1 pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...68720 68720-AAV1 pAAV-hSyn1-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA Biosensor Tobias Bonhoeffer, Mark Huebener...Loren Looger AV-1-PV2816 100835-AAV1 pAAV.CAG.Flex.GCaMP6f.WPRE.SV40 Biosensor GENIE, Douglas Kim AV-1-PV2817...

  18. Brain Initiative Collection

    Type
    Collection
    ...158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker...element Gordon Fishell 83899-AAV1 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...element Gordon Fishell 83899-AAV9 pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons... Gordon Fishell 83899-AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 GCaMP6f expression in forebrain GABA-ergic interneurons...111261-AAV9 pAAV-hSynapsin1-axon-GCaMP6m For enriched expression of GCaMP6m in axons Lin Tian 111262-AAV1...AAV1 pAAV-hSynapsin1-axon-GCaMP6s For enriched expression of GCaMP6s in axons Lin Tian 111568-AAV5 pZac2.1...

  19. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...OPEN gAGCATCACCtcgctGGAGGTGAGc PTRF/Cavin1 OZ513 and OZ514 OPEN OPEN gACAGCCGTCtgcatctGGAGGTGCCc pitrm1...OPEN OPEN cACCTGCAGCagattGAAGAAGAGg cftr OZ517 and OZ518 OPEN OPEN gTGCAACCGCagctttTAGGACGGAt fox1 (a2bp1l...OPEN OPEN tTACTTCTGCaatgatGTGGTGGCTc vipr1 OZ559 and OZ560 OPEN OPEN gGCCATCAGCtcgcattGGAGAAGTAg NRF2b (...CODA OPEN cAACCTCAGCATAAAGCTGGAGGAg psen2 OZ577 and OZ578 CODA OPEN cGGCATCTCCAGAGCCCGAGGCGGACc zgc:162148...CODA cTGCACCTTCACAAAAGGCCGCTGGCt LOC566040 OZ587 and OZ588 OPEN OPEN cAGCACCAGCCAGCCCAGAGTTGGGGt yap1 ...hydroxylase (dbh) OZ525 and OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527...aGCCATCATCaggttcaGGATGAGCCc Kif6 OZ537 and OZ538 OPEN OPEN aAGCTTCCTCttcattgGCTGACTGCa ldr (low density lipoprotein receptor...

  20. Caltech Systemic Capsids

    Type
    Collection
    ...Cre-dependent GCaMP Kim , GENIE 104487 pGP-AAV-syn-jGCaMP7s-WPRE Syn Calcium sensor GCaMP Kim , GENIE 104488... pGP-AAV-syn-jGCaMP7f-WPRE Syn Calcium sensor GCaMP Kim , GENIE 135632 pAAV-S5E2-GCaMP6f E2 regulatory...104491 pGP-AAV-syn-FLEX-jGCaMP7s-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104492 pGP-AAV-syn-FLEX-jGCaMP7f-WPRE...-AAV-syn-FLEX-jGCaMP7f-WPRE Syn Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE...pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG Calcium sensor, Cre-dependent GCaMP Kim , GENIE 104496 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE... Kim , GENIE 162375 pGP-AAV-syn-jGCaMP8m-WPRE Syn Calcium sensor GCaMP GENIE Optogenetics 26973 pAAV-hSyn-hChR2...regulatory element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor...
Showing: 21 - 40 of 49 results