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  1. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    ...). Best uses Finding papers frequently cited by others. This is a great hack for finding relevant papers...Finding research papers is not particularly hard. There are millions of them. The real challenge is finding... finding relevant papers. The latest installment of the Early Career Researcher Toolbox will highlight...highlight four tools for finding journal articles related to your actual interests while also staying on top ...PubMed PubMed is the classic search engine for finding papers. It’s been around since 1996, but is getting...be summarized in 280 characters! Using Twitter to find papers  You’ll need to start following relevant ...accounts in order to turn Twitter into a paper-finding-machine. There are four general types of accounts...

  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...same information in our search indices in order to support different kinds of matches: exact words, partial...Fortunately, we’ve just made it easier for you to find what you need quickly.  Through the years, we have... a result, resources on the website were hard to find and many remained undiscovered.  To remedy this,...Now, let’s take a look at the least visible part behind these changes: the backend technology that powers...powers the new website search. Tackling findability from multiple angles    We assembled a cross-team team...needed to improve more than that: the general “findability” of the information on the site. Different users...users have different strategies for finding what they need, and we wanted to support all of them — searching...

  3. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...MIcrobiology Pages The problem: Host binding proteins are hard to find Sequencing and cloning phage DNA can...evolution of their bacterial prey. Currently, receptor binding proteins (RBPs, the host recognition factors of...study. These are highly useful for the biotech industry, which is in the process of capitalizing on phage..., phages represent a reservoir of carbohydrate binding proteins waiting to be explored and exploited. ...phage genome to be probed for proteins with host-binding activity without the need for genome sequencing...sequenced genome that encode for functional cell-binding proteins in the (all-too-common) event that homology-based...coli and subsequent proteins screened for cell-binding abilities (6). This can be accomplished using a...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...cost a fortune for a successful outcome. How do I find a coach? Start with referrals. Are there scientists...referrals. There are bad coaches out there, so finding a good one that is a style match for your needs... of transitioning a scientist from academia to industry, you might want to consult the Cheeky Scientist...We Turn Academics into Confident and Successful Industry Professionals”. This larger shop might appeal ...excellent in coaching for communication skills. Industry transition is also an area of specialty for Michelle...Resume That Gets Seen  Guide Your PhD with An Individual Development Plan Learn a Little about The Academic...Check out Our Career Page for Recent Job Openings Find Plasmids to Expedite Your Research   ...

  5. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    ...What's a lab group in industry? The collaborative nature of working in industry and how delegating can...Crystal’s exciting career path, to gain insight into industry research, and to get advice on how to succeed ...Advice to scientists who are planning on working in industry. The importance of recognizing and leveraging ...adapting to needs as they arise. Tips on applying for industry jobs and deciphering job listings. Mobility between...Podcast.      Additional Resources from Addgene Find more management resources in our Mentoring for Scientists...

  6. CRISPR Kinome Libraries Available: Pooled and Individual Plasmid Formats

    Type
    Blog Post
    ...and individual plasmid formats A first for a library at Addgene, you can also order the individual lentiGuide...all-in-one (lentiCRISPRv2, expressing both Cas9 and an individual gRNA from this single vector) and guide-only ...in the first subset of the pooled library. The individual plasmids will be useful when screening for phenotypes...themselves to screening in a pooled setting. The individual plasmids will also be useful for imaging or gene...expression assays necessitating the screening of individual plasmids separately. Additionally, this set can... can be used for cherry-picking individual gRNAs that score as hits in pooled screens, facilitating more...follow-up. We hope the biological community will find these reagents to be useful. Happy screening! Many...

  7. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    ...has been referenced in other publications, you'll find a link to the list of citing articles under the ...article list you can click on the PubMed link to find the article abstract and more. We've also included...into a previously deposited vector - then you'll find the plasmids listed below the citation. An example...Barcoding cells using cell-surface programmable DNA-binding domains" publication.  If you want to see this...identify relevant protocols and experimental data Find other related plasmids in the "Associated Plasmids... Happy researching! If you need any assistance finding plasmids on Addgene's website or would like to ...

  8. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

    Type
    Blog Post
    ...available to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists...University became openly available. Find plasmids available to industry scientists To make plasmid sharing...available more broadly: to scientists in academia and industry. As a result over 90% of Addgene’s COVID-19 resources... to Addgene’s new approach of working with the industry-based scientific community. Conversations with...to the scientific community - both academic and industry scientists. This partnership complemented each...we have other plasmid collections available to industry? Over the past 8 years, several organizations ...sharing more open for academic and industry scientists, we’ve worked with many scientists, tech transfer offices...

  9. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type
    Blog Post
    ...Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links to CRISPR references ...bison wanders into the campsite, leave your samples behind! Paul and Anastasia (Potanina) Neddersen characterized...thermostable between 31-100°C. They published their finding in a bioRxiv article and in PNAS. Two other thermostable...the van der Oost lab. Phylogenetic comparison indicates that like GeoCas9 and ThermoCas9, IgnaviCas9 is...lavamentivorans, whose optimal growth temperature is 30°C. Find IgnaviCas9 here! What can you do with IgnaviCas9...

  10. Find and Share AAV Data with Addgene's New AAV Data Hub

    Type
    Blog Post
    ...of the juicy and important details that you won’t find in the final publication and we have already collected... AAV Data Hub grows, we hope to mine the data to find patterns that we can share with you about promoters...

  11. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    ...depositing plasmids pre-publication Find molecular biology protocols Find eBooks on plasmids, CRISPR, viral... sentences that I found hard to understand or individual words that are not appropriate for the given ... attention to details and use a checklist for individual sections of the paper (see figures). After finishing...speaking to a colleague in the office next door. So be kind, helpful, and thorough in reviewing the paper of...Magdalena Julkowska did her Ph.D. on salt-stress induced responses in Root System Architecture of Arabidopsis...Prof. Mark Tester, where she focuses on (1) salt-induced changes in root-to-shoot ratio in Arabidopsis, ... Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility, peer review, and Nature, 1945–1990...

  12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    ...homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia...Addgene.org Visit our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab...reprogramming factors- OCT4, SOX2, KLF4, and C-MYC - that induce a differentiated somatic cell to revert back to...Addgene’s stem cell page. On this page, you can also find a table with a list of methods and the species they... hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here. ... cases where the objective is to fix a disease-inducing genetic mutation in a patient, trying to transdifferentiate...PubMed PMID: 16810240. 2. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...

  13. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...you’ll find Addgene’s newly launched search engine results much better. Here, I wanted to find a specific...plasmids with more than the indicated number of requests Availability to Industry: Restrict search to plasmids...Finding the best plasmid for your research can be a daunting challenge. With over 80,000 deposited plasmids...plasmids from around the world, it can be hard to find your favorite gene, promoter, serotype, or a myriad...articles. From paper to plasmid Authors regularly indicate that their plasmids are available from Addgene... show the range of different terms we can use to find materials from this publication. Searching for the... the title of a paper works You can also find the associated plasmids through the article’s PubMed ID...

  14. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type
    Blog Post
    ...disadvantages led to the creation of induced pluripotent stem cells or iPSCs. Induced pluripotent stem cells iPSCs...Addgene.org Visit Our Stem Cell Page Find Plasmids for Reprogramming Find Plasmids for Differentiation ...works led to a Nobel Prize for Dr. Yamanaka in 2012. Find plasmids for generating iPSCs iPSCs and ESCs closely...: 17989069. 8. Takahashi, K. and S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic...PubMed PMID: 16904174. 9. Takahashi, K., et al., Induction of pluripotent stem cells from adult human fibroblasts...72. PubMed PMID: 18035408. 10. Yu, J., et al., Induced pluripotent stem cell lines derived from human ...Medvedev, S.P., A.I. Shevchenko, and S.M. Zakian, Induced Pluripotent Stem Cells: Problems and Advantages...

  15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...frogs, mice, and even humans. It binds to a specific set of DNA-binding transcription factors to repress... from. Let’s take a look at some of the stories behind the names. We also have many plasmids in our repository...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid however...terrible fate when she pricked her finger on the spindle of a spinning wheel but also a biological transposon...vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the name but some of our...properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name but the larval...Plasmids 101 blog series Resources on Addgene.org Find plasmids for your research Deposit plasmids at Addgene...

  16. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...fused to a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin...it’s briefer time scale and its inducible and reversible nature. Find the LADL plasmids at Addgene Kim...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...Article contributed by Alyssa Cecchetelli Auxin Inducible Degradation (AID) uses plant hormones called auxins... the AID technology (Yesbolatova et al., 2019). Find the mAID tagging toolkit at Addgene Yesbolatova ...specific PAM (protospacer adjacent motif) in order to bind the target sites for editing. PAM sequences can ...they also fused the SpCas9-NG with the activation-induced cytidine deaminase  Target-AID-NG, which mediated...

  17. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast... genetically encoded voltage indicators. To expand the voltage indicator toolbox, Ed Boyden’s lab developed...small scaffold proteins to identify novel binding proteins that bind to either the light- and dark- states...cPYP), and plasmids that encode the protein binders (which bind to either the light- or dark- state of the..., because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid tools ...for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis JM, et al. ...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...

  18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...the ribosome binding site is inaccessible. When the target RNA is present, however, it binds to the switch...deposits and we hope they'll make it easier for you to find and use the plasmids you need. If you'd ever like...the dsRNA leading to increased RNAi efficiency. Find the T444T plasmid here! Strum A, et al. Nucleic ...an option, including Drosophila and C. elegans.   Find the TurboID plasmids here! Branon et al. Nat Biotechnol...switch and exposes its ribosome binding site, allowing for the gene on the toehold switch to be expressed...display and deep sequencing, revealing that they bind to a much larger subset of protein motifs than previously...biophysical study involving protein stability or binding experiments. Potential experiments that one might...

  19. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...fluorescent cAMP indicator (Flamindo2) Genetically-encoded yellow fluorescent cAMP indicator with an expanded...protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator applicable to optogenetics...Calcium Indicators (Constitutive or Cre-dependent) jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia...calcium indicators for optical imaging (GECOs), including blue, green, red, and ratiometric indicators An ...calcium indicator for fluorescence using HaloTag ligands A modular chemigenetic calcium indicator enables...fluorescence indicator of K+ for optical imaging (GINKO) Genetically encoded fluorescent indicators for imaging...Multicolor palette of ATP indicators (MaLion) RGB-color intensiometric indicators visualize spatiotemporal...

  20. Antibody Guide

    Type
    Collection
    ...structure are responsible for binding the antigen, and are called the antigen binding fragments (Fab). The tips...recognizing and binding to epitopes on antigens, they are also capable of recognizing and binding epitopes on...single individual, giving a high probability that an antibody will be able to recognize and bind to any... and binds to the protein of interest. Signal strength is determined by how many antibodies bind the protein...detection antibody is added and binds to the bound protein. If using the indirect sandwich method, a conjugated...? Will you be using a direct or indirect detection method? If indirect, do you already have secondary ...tools used in the lab to specifically and tightly bind to other proteins. Antibodies, produced as part ...
Showing: 21 - 40 of 1076 results