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We narrowed to 208 results for: Kars

Showing: 21 - 40 of 208 results

  1. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...applications. Find a chromoprotein for your experiment! Eukaryotic chromoproteins for bacterial synthetic biology...common chromoproteins used in the lab come from eukaryotes, they can be difficult to directly use in bacteria.... https://doi.org/10.1007/bf00428249 Chan MCY, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki...Forster, A. C. (2018). Engineering a palette of eukaryotic chromoproteins for bacterial synthetic biology...

  2. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...described below. IRES Elements Translation in eukaryotes usually begins at the 5’ cap so that only a single...it promotes translation of the viral genome in eukaryotic cells.1,2 Since then, a variety of IRES sequences...employed by scientists. 2A cleavage is universal in eukaryotic cells, and, although some scientists report close...References: 1. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus...

  3. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...Many thanks to our guest blogger, Aneesh Karve! Aneesh Karve is CTO at Quilt Data. Quilt is a collaborative...This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally published...for genomics. In this article, Quilt CTO Aneesh Karve, shows how to design experiments that work anywhere...

  4. CRISPR Antimicrobials

    Type
    Blog Post
    ... like C. difficile. In 2014, Citorik et al. and Bikard et al. developed sequence-specific, antimicrobial...phagemids were cytotoxic only for resistant bacteria. Bikard et al. used a phagemid system to target virulent... the phagemid or received a defective phagemid; Bikard et al. did not observe any mutations at the target... Find plasmids from this paper at Addgene. 2. Bikard, David, et al. “Exploiting CRISPR-Cas nucleases...

  6. What's New in CRISPR - September 2019

    Type
    Blog Post
    ...genome-wide inhibition library from David Bikard’s lab. The Bikard library contains ~93,000 unique gRNAs ...Read the paper in Nature Communications Find the Bikard E. coli Genome-wide Inhibition Library CRISPR ...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...expression systems have been improved for both eukaryotic and prokaryotic expression in recent years, with many...cell-free expression of proteins in both prokaryotic and eukaryotic cell extracts (Gagoski et al 2015). He...sequence bypasses the 5′ mRNA cap required for eukaryotic systems and instead directly engages the ribosome...efficiently blocks read-through transcription in eukaryotic cells. pXPG contains the Luc+ luciferase gene...

  8. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...Optogenetics Karl Deisseroth New serotype pAAV-hSyn-hChR2(H134R)-EYFP AAV2 Optogenetics Karl Deisseroth... serotype pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE...

  9. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...learn more! Background: Argonaute and NgAgo In eukaryotes, Argonaute proteins play an important role in... RNA. Argonaute proteins are also present in prokaryotes, and some of these proteins protect against invading...., et al.  “DNA-guided DNA interference by a prokaryotic Argonaute.” Nature. 507(7491) (2014):258-61. ...

  10. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...single RFP-labeled osk and Oskar protein were detected at the posterior pole. Oskar protein levels were negatively...examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK reporter was...

  11. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    ...AAVrg pAAV-Ef1a-DIO EYFP Karl Deisseroth 11447 AAV5 pAAV-Ef1a-fDIO mCherry Karl Deisseroth 112677 AAV2...pAAV-hSyn-DIO-EGFP Bryan Roth 114472 AAVrg pAAV-hSyn-mCherry Karl Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell...

  12. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...collaborations. Summarized by Professor Michael Karin from UCSD: “The problem with cancer research is ...studies! This was followed by a talk from Professor Karin about alcohol and obesity as driving factors for.... Todoric, Jelena, Laura Antonucci, and Michael Karin. "Targeting inflammation in cancer prevention and...

  13. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...occurring restriction modification systems that prokaryotes use to protect themselves from foreign DNA. The...degraded by restriction endonucleases. A given prokaryote typically has genes encoding one or a few restriction...Impact on cloning and digests Although not all prokaryotic DNA is methylated to the same level, the potential...

  14. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...efficient tRNA processing is common to prokaryotes and eukaryotes. Though the plasmids made by Xie et. ... or pre-tRNAs are cleaved by RNase P and Z in eukaryotes (E in bacteria), and only a few elements of tRNAs...

  15. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...Many thanks to our guest blogger Oskar Laur from Emory University. Oskar Laur, PhD runs Emory University...This post was contributed by Oskar Laur, head of the custom cloning core at Emory University, and Paolo...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...whether and how organisms respond to magnetic fields (Karki et al., 2021; Kattnig et al., 2016), and some previous... cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka et al.,... andrewgyork.github.io/gfp_magnetofluorescence. Karki, N., Vergish, S., & Zoltowski, B. D. (2021). Cryptochromes...

  17. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ...//doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS, Shmakov S, Koonin EV (2017) Recruitment of...Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F (2019) RNA-guided DNA insertion...doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova KS, Koonin EV, Zhang F (2020) Response to Comment...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...codon optimize genes from eukaryotic organisms for expression in prokaryotic systems or yeast (Lanza et...base-pairing mechanism for translation initiation in eukaryotes. Nat Struct Mol Biol 13:30–34 . https://doi.org...

  19. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ... at Addgene. Kleinstiver BP, Prew MS, Tsai SQ, Topkar VV, Nguyen NT, Zheng Z, Gonzales APW, Li Z, Peterson...Gootenberg JS, Kriz AJ, Zetsche B, Shalem O, Wu X, Makarova KS, Koonin EV, Sharp PA, Zhang F (2015) In vivo... B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der...

  20. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    ...helps scientists achieve a high titer (reviewed in Joglekar and Sandoval, 2017). This high titer is one of...tropism, and advantages and disadvantages: Table 1 (Joglekar et al., 2017)  Table 1 (Gutierrez-Guerrero et ...Viruses 12:1016 . https://doi.org/10.3390/v12091016 Joglekar AV, Sandoval S (2017) Pseudotyped Lentiviral Vectors...
Showing: 21 - 40 of 208 results