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We narrowed to 178 results for: Lta;

Showing: 21 - 40 of 178 results

  1. Antibodies 101: Flow Cytometry Controls

    Type
    Blog Post
    ...color controls are used to help you set up your voltages and can also be helpful for compensation. You ... cells. This can be achieved by adjusting your voltage using your single color controls (Figure 1A), instead... using beads and not experimental samples, the voltages and compensation you set from your single color...analysis rather than for setting up your initial voltages and gates. Ashlyn has a research background in...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... wider gel comb and running the gel at a lower voltage. You will want to have enough space around each...DNA bands include: a) running the gel at a lower voltage for a longer period of time; b) using a wider gel...

  3. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ... multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality of the...of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex control via scRNA...Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable control of gene...

  5. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...experiences, she realized her niche as a grant consultant to faculty writing research grants, especially...full-time job or want to be an independent grant consultant, building your area of expertise will help you...to mentor and coach you. There are many grant consultants who offer grant training, mentoring and coaching...

  6. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...downstream of a functional promoter (gene traps). The resultant gene targeting system could be used to routinely...at once. Therefore, applications that require simultaneous modification of all alleles of a gene, or which...which require modifying multiple genes simultaneously in the same cell, are more appropriate for a CRISPR-based...

  7. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...approach relies on the incorporation of light-oxygen-voltage (LOV) sensing domains from algae into chimeric ...integrating DNA sequences either sequentially or simultaneously. This method, called by the authors the “Clonetegration...repeat-variable di-residue (RVD) module plasmids and simultaneously increases the success rate of module assembly...in place of Brainbow’s Cre/loxP, allowing for simultaneous use of the two systems in different tissues ...

  8. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...Essentially, any method that results in the bulk of resultant fragments falling between 1 and 3 kb results in...modifications to DNA bases.  Ligate and transform your resultant plasmid libraries into chemically competent E....DNA directly from the colonies. Transform the resultant plasmid DNA into E. coli BL21 (DE3) cells (these...

  9. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...assembly, and up to 8 gRNAs may be expressed simultaneously. Vectors for both transient expression and ...small insertions. Multiplexing of two targets simultaneously is possible with efficiency >70%. CRMAGE is... delivered on a different plasmid and allows simultaneous multiplex editing of six different genes. This...

  11. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  12. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines of mice. The... To operate both types of implanted fibers simultaneously, a mini-Bluetooth system named NeuroStack was...

  13. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

  15. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ...follow multiple fluorescently tagged species simultaneously. To do so with fluorescent protein labels requires...of fluorescent proteins, imaging four colors simultaneously should be pretty straightforward. Thank You...

  17. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ... et al., 2008), and monitor neuronal activity (voltage sensors), to name a few.   Find Many FRET-Based... permit the use of multiple FRET biosensors simultaneously would further increase the amount of correlated...

  18. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...CEPIA (additional versions are available to simultaneously measure calcium changes in the ER, cytosol ...fluorescence to a red fluorescent state only by simultaneous (1) user-controlled illumination with violet...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...for instance, the challenge of attempting to simultaneously and selectively induce both cutting and regulation... targeting a different set of loci to induce simultaneous cutting and transcriptional activation or repression...
Showing: 21 - 40 of 178 results