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  1. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...cytochrome P450 genes discovered in Drosophila melanogaster (Gilbert, 2004). Mutations in these genes are...mediate steroid hormone biosynthesis in Drosophila melanogaster." Molecular and cellular endocrinology 215.1...

  3. Antibodies 101: Isotypes

    Type
    Blog Post
    ...Garland Science; 2001. Structural variation in immunoglobulin constant regions. https://www.ncbi.nlm.nih....Science; 2001. The distribution and functions of immunoglobulin isotypes. Available from: https://www.ncbi....

  4. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...develop a way to quantify the take rate of mouse xenografts after cell implantation. Their goal was to figure...drug treatment in cell line and primary tumor xenograft models. She hopes that the library can be used...

  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibodies used for detection bind to non-target immunoglobulins naturally present in the tissue sample. There...antibody fragments to block the endogenous immunoglobulins and reduce the background staining but they...

  6. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    ...transgene expression (Interestingly, the strong immunogenicity of AdVs is what makes them ideal candidates...potential for the generation of RCA and reduced immunogenicity. In addition to E1/E3, non-structural genes...

  8. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ... abandoned early on due to its considerable immunogenicity and its transient expression. LV results in...to cause any disease in humans, and its low immunogenicity is critical when systemic delivery of high ...

  10. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... for in vivo gene delivery due to their low immunogenicity and range of serotypes allowing preferential...Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA (2019) CasX enzymes comprise a distinct...

  11. Viral Production at Addgene

    Type
    Blog Post
    ...contamination in vector preparations can alter the immunogenic properties of the final product, particularly...plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation, the...

  12. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets...

  13. Viral Production

    Type
    Collection
    ...contamination in vector preparations can alter the immunogenic properties of the final product, particularly...plasmid purification protocol. To minimize the immunogenic properties of the final vector preparation, the...

  14. Cre-lox system

    Type
    Collection
    ...Youle 59720 Nanog-CreER targeting construct Cre-ERT2; Targeting vector for Nanog locus mNanog Mammalian ...

  17. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP reporter. Minicircles are removed from... plasmids (including reporters and RNAi) for:  NANOG  OCT4 SOX2 MYC KLF4 LIN28 Check out these plasmids...

  18. Our New Antibody Mascot is....

    Type
    Blog Post
    ...Lin, or Blottie? Which name best fits our new immunoglobulin friend? Please help us decide by voting for...
Showing: 21 - 40 of 105 results