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We narrowed to 59 results for: Neb

Showing: 21 - 40 of 59 results

  1. Plasmid Cloning by PCR

    Type
    Blog Post
    ...DNA efficiently at the end of a linear piece (see NEB for more information). Thus, we recommend that you...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...England Biolabs (NEB) #R0552S EcoRI NEB #R0101S NEB buffer 1 NEB #B7001S NEB buffer 2 NEB #B7002S T4 DNA...DNA ligase NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012...concentration, use 1 μL) 2 μL 10x NEB T4 DNA ligase buffer 1 μL NEB T4 DNA ligase add ddH 2 O to bring...catalog # DNA Miniprep Kit Qiagen #27104 EcoRI NEB #R0101S NcoI NEB #R0193S Agarose Sigma #A9539 D.2 Screening...enzyme manufacturer's resources, such as NEB’s online tool NEBcloner (Link opens in a new window) , to help...: 5 μL Forward oligo 5 μL Reverse oligo 5 μL 10x NEB buffer 2 35 μL ddH 2 O Incubate for 4 minutes at ...TRC-cloning vector (maxiprep or miniprep DNA) 5 μL 10x NEB buffer 1 1 μL AgeI add ddH 2 O to bring to 50 μL ...

  5. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...restriction enzymes, visit (Link opens in a new window) NEB . Restriction enzyme digestion is commonly used in... in a new window) double digest finder tool from NEB . In a 1.5mL tube combine the following: DNA Restriction... sticky ends. See (Link opens in a new window) NEB's compatible cohesive ends chart . If you cannot find...restriction sites. See (Link opens in a new window) NEB's table of methylation sensitive restriction sites...concentration. Learn more at (Link opens in a new window) NEB's website about star activity . If you are digesting...

  7. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...blog High Complexity Golden Gate Assembly with NEB’s DAD Interview with Nicola Patron on MoClo Plasmids...

  10. Tips for Technical Support Calls

    Type
    Blog Post
    ...questions (besides Addgene, I know both Abcam and NEB have Ph.D.s answering emails and phones). We can’...

  14. Hot Plasmids - August 2020

    Type
    Blog Post
    ...expressed in SHuffle T7 Express competent E.coli (NEB) using IPTG induction. To biotinylate the nanobodies...

  15. Viral Production at Addgene

    Type
    Blog Post
    ...viral production are propagated in the endA-mutated NEB Stable strain of E. coli. In addition, plasmids are...

  17. Gibson Assembly Protocol

    Type
    Protocol
    ...time. Resources and References New England Biolabs (NEB) (Link opens in a new window) sells DNA Assembly ... also sell ET SSB (Link opens in a new window) . NEB has other resources, such as a primer design tool...Assembly kits and master mixes, including NEBuilder HiFi (Link opens in a new window) and Gibson Assembly (Link...
Showing: 21 - 40 of 59 results