We narrowed to 162 results for: PIR

1. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

   Type

   Blog Post

   ...the other hand some software tools make use of empirically derived and/or prior knowledge to inform on the... Hsu et al., 2013) used a combination of both empirical and approximate methods. The details of their ...“high mismatch limit”, “approximate design”, “empirical design”, “single-PAM design”, and “multi-PAM design...

2. #DeckTheLab to Win a Tasty Winter Treat This Holiday Season

   Type

   Blog Post

   ... Here at Addgene we’re getting into the holiday spirit and we hope you are, too! Bluegene is eager to ...causing him some difficulty… To give him some inspiration, we’d love to see how you’re decorating your ...

3. #Deckthelab Contest Winners 2017

   Type

   Blog Post

   ...away all of our holiday decorations, our holiday spirit remains. We would like to thank everyone who participated...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

4. Congrats to the 2019 Deck The Lab Winners

   Type

   Blog Post

   ...The holidays may be over, but signs of holiday spirit still linger (despite the unseasonably warm weather... Miller lab annual #DeckTheLab. Getting in the spirit for the holiday season. Santa frog is coming to...

5. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

   Type

   Blog Post

   ...Collins labs at the Wyss institute for Biologically Inspired Engineering. Alex is the first MD PhD we've had... - 13:21 : The Wyss Institute for Biologically Inspired Engineering and why Alex decided to work there...

6. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

   Type

   Blog Post

   ... regularly be paired with a variety of science-inspired friends. As you can see on the homepage, Blugene... you can find what you need and feel happy and inspired to tackle your next science challenge. If you ...

7. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

   Type

   Blog Post

   ...the presence of scientific heroines is always inspiring. I was especially excited to attend this event...genome. Their award acceptance addresses were inspiring! We also got to hear more from them during a panel...

8. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

   Type

   Blog Post

   ...bacteria and archaea to regulate their buoyancy. The Shapiro lab recently discovered that gas vesicles also ...Lakshmanan, A., Farhadi, A., Kumar, S.R., Nety, S.P., & Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive...

9. With Our New Viral Service, We're Taking CRISPR Further!

   Type

   Blog Post

   ...researcher at the Wyss Institute for Biologically Inspired Engineering and member of the Addgene Advisory...Church lab at the Wyss Institute for Biologically Inspired Engineering and the Zhang and Doench/Root labs...

10. Cultivating Community Science at BosLab

    Type

    Blog Post

    ...initiated a soil microbiome project. This project was inspired by previous city-wide genomic studies and our ...DIY bio If you’re not in the Boston area but are inspired to get involved, check out the local groups page...

11. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    ... a radioactive tracer. As a solution, Mikhail Shapiro’s lab has developed four acoustic reporter gene ...water from entering their hollow interiors. The Shapiro lab discovered in 2014 that these gas vesicle proteins...

12. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type

    Blog Post

    ...your plasmids in: DH5α, NEB Stable, ccdB Survival, Pir1, or other. Whenever possible, we propagate plasmids...contain the R6Kγ origin of replication, choose the Pir1 strain. If your plasmid cannot be propagated in ...

13. Hot Plasmids February 2024

    Type

    Blog Post

    ...for your next experiments, they will also likely inspire further-improved versions. Thanks to Christophe...Leterrier, whose FocalPlanes piece on mStayGold inspired and informed this section.  Find mStayGold plasmids...

14. The Materials Science of Optogenetics Experiments

    Type

    Blog Post

    ...stimulation parameters must also be determined empirically but it’s best to try those that have already ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

15. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    ...doing this with very little money. So when did inspiration strike us so hard that we upended our lives and...in fact, our color changing flower project was inspired by a Facebook comment.  In our day job, we worked...

16. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ... Patron: I started my Twitter account recently inspired by an academic in literature who gave a talk to...female scientists whose tweets I find really inspirational. They share experiences, articles about women...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...to the N- and C-terminal end of each PIP and empirically test which combination provides the best fluorescent...constructing appropriate controls may requires that you empirically test a number of mutants to identify those that...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    ... just be sure to check that your RNase has not expired (or been forgotten).    Improving read accuracy...purification protocol. Are any of your materials expired? Did you pipette carefully? Remember to discard...

19. Plasmids 101: Broad Host Range Plasmids

    Type

    Blog Post

    ...spp., Aliivibrio salmonicida, Anabaena spp., Azospirillum braziliense, Azotobacter spp., Bordetella spp...spp., Rhizobium spp., Rhodopseudomonas spp., Rhodospirillum spp., Shewanella spp., Thiobacillus spp., Xanthomonas...

20. CRISPR Activation: A Practical Guide

    Type

    Blog Post

    ...researchers at the Wyss Institute for Biologically Inspired Engineering. CRISPR/Cas9 is an enormously plastic...researcher at the Wyss Institute for Biologically Inspired Engineering.     Alejandro (Alex) Chavez is a ...