We narrowed to 50 results for: PRS
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TypeBlog Post...article in the GSA’s blog Genes to Genomes about a TMPRSS2 plasmid from Roger Reeve’s lab as an example). ...
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CRISPR Cheat Sheet
TypeBlog Post...CRISPR including: Base editing Prime editing Anti-CRISPRs … you name it! So get excited and download the... -
Fueled by Coffee at #SfN14
TypeBlog Post...tools into neuroscience experiments. Speaking of CRISPRs, we were excited to have Feng Zhang (of the Broad... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog Post...therapy. SB is a valuable tool and an alternative to CRISPRs for genome engineering whose use is only likely... -
CRISPR/Cas9 FAQs Answered!
TypeBlog Post...design tools Need more questions answered about CRISPRs? Check out the full list of 16 FAQs answered by... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog Post...vector (pcDNA3) and a bacterial expression vector (pRSETB). Fabritius A, et al. Cell Chemical Biology 2018... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...you can use to express the iGluSnFR3 variants: pRSET: Bacterial expression vector, use with IPTG induction... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...plasmids express these and other targets such as TMPRSS2, FURIN, and more. Recently, scientists used the... -
Plasmids 101: Protein Expression
TypeBlog Post...amounts of protein in bacterial cells: the pET, pRSET, Gateway pDEST, and pBAD vectors for example. Protein... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...induced by anhydrous tetracycline. Three new anti-CRISPRs were identified that inhibit the S. aureus Cas9... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...Appendix DNAse Hypersensitive Sites and Intergenic CRISPRs Yours truly generated the DHS data set by starting... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog Post...Our CRISPR Featured Topic Page Learn about Anti-CRISPRs Multiplex Genome Editing with Cpf1 Resources on... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...designed to help explain the basic principles driving CRISPRs and genome editing technology. Links: Zhang Lab... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post....114.172361 Want to learn more about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn ... -
Special Delivery: Fluorophore Targeting for FRET Studies
TypeBlog Post...pCMV-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive... -
The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs
TypeBlog Post...too slow for practical use. The R based package CRISPRseek offers great utility and coupled with Bioconductor... -
Genetic Code Expansion
TypeCollection...Bacterial TCG and TAG Jason W Chin 174718 pRSF-G1mCNPRS G1mCNPRS M. archaeon L-3-(2-cyano-4-pyridyl)alanine...mCNP) Bacterial TAG Thomas Huber 174719 pRSF-G1pCNPRS G1pCNPRS M. archaeon L-3-(2-cyano-5-pyridyl)alanine...TAG Simon Elsaesser 140019 pAS_4xEcoLeuT(CUA)_AnapRS AnapRS E. coli 3-(6-acetylnaphthalen-2-ylamino)-2-...nhpSer SepRS M. maripaludis non-hydrolyzable phosphoserine Bacterial TAG Ryan Mehl 207516 pRSF-G1(7AW)...Badran 209744 pAB228v TrpRS S. cerevisiae Bacterial Ahmed Badran 209745 pAB228v6 TrpRS S. cerevisiae Bacterial...Removed endogenous EcTrpRS/ttRNA pair (knocked out trpS and trpT genes). Expresses yeast TrpRS/tRNA pair instead... -
COVID-19 Resources
TypeCollection...SARS-CoV-2 . TMPRSS2 - Hoffmann M, et al, 2020, Cell SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is...2014, J Virol. TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry...of SARS-CoV-2 is primed by the serine protease TMPRSS2. Image credit: Maya Kostman for the Innovative ...role in coronavirus infection, such as ACE2 and TMPRSS2, which are involved in the entry of SARS-CoV and...entry of SARS-CoV and SARS-CoV-2 viruses. ( 1 ) TMPRSS2 - a serine protease that primes the SARS-CoV-2 ... -
Neurodegeneration Plasmid Collection
TypeCollection... Parkinson's Michael J Fox Foundation MJFF 40586 pRS-45 TBP His, yTAND12 T7 Parkinson's Harald Schwalbe...Elizabeth Fisher 27447 pRS416Gal TDP43 WT YFP TARDBP YFP GAL1 ALS Aaron Gitler 27448 pRS416 Gal G294A YFP TARDBP...Aaron Gitler 27449 pRS416 Gal M337V YFP TARDBP YFP GAL1 ALS Aaron Gitler 27450 pRS416 Gal Q331K YFP TARDBP... Aaron Gitler 27458 pRS416 Gal TDP43 WT TARDBP GAL1 ALS Aaron Gitler 27459 pRS416 Gal TDP43 G294A TARDBP...Aaron Gitler 27460 pRS416 Gal TDP43 M337V TARDBP GAL1 ALS Aaron Gitler 27461 pRS416 Gal TDP43 Q331K TARDBP...ALS Aaron Gitler 27466 pRS426 Gal TDP43 TARDBP GAL ALS Aaron Gitler 27467 pRS426 Gal TDP43 GFP TARDBP GFP...hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post... of luciferase-fluorophore pH fusion reporters, pRSETb-pHlash and pcDNA3.1+-pHlash, that use Bioluminescence...