We narrowed to 27 results for: PUR ALPHA-1

...into account, the digest pattern depicted in Lane 1 would be expected for a XbaI/NotI digest. The program... these enzymes naturally occur in bacteria for purposes other than cloning or confirming plasmids. It ...cleave DNA. Common lab E. coli K12 strains such as DH5alpha contain 3 methylases that recognize and methylate...will cut? Whether performing a digest for cloning purposes or for diagnostics, we suggest double checking...–/dcm– strain of E. coli such as JM110 and re-purifying it. These specialized E. coli strains have been...

...faster. Years of science, done in weeks Figure 1: Sending out 80 FedEx envelopes in a single day --... 2xStrep-tag for easy detection and affinity purification. All but one are in a pLVX vector background... if you need to. Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection...selection. Expression is driven by a fairly strong EF1alpha promoter. We have verified expression of correctly-sized...

...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important... life is the lab notebook. Lab Archives is a multipurpose application that allows you to share your data...restriction site to a primer, you only need to skim the alphabet on the side of the screen to find the sequence...

...levels of DnaA to get the job done.   Figure 1: DNA melting by DnaA and IHF is the first step of ...plasmids may result in low yields during DNA purification. On the other hand, they also impose a lower... Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot...

...has error rates that range from roughly 1 per 500bp to roughly 1 per 10 million bp depending on the polymerase...you can transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies,...Vector by Gel Purification Run your digest DNA on an agarose gel and conduct a gel purification to isolate...isolate the DNA. When running a gel for purification purposes it is important to have nice crisp bands and ...and grow overnight cultures for DNA purification. After purifying the DNA , conduct a diagnostic restriction...TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA-3’. Experimental Procedure Run PCR and Purify the PCR Product Run PCR to amplify your insert ...

...you can transform 1-2μl of your ligation reaction into competent cells such as DH5alpha or TOP10. If using...recipient plasmid to insert ratio of approximately 1:3. Since the number of base pairs for each varies,...vector by gel purification Run your digested DNA on an agarose gel and conduct a gel purification to isolate...isolate the DNA. When running a gel for purification purposes it is important to have nice crisp bands and...and grow overnight cultures for DNA purification. After purifying the DNA , conduct a diagnostic restriction...digest is a commonly used lab technique. For the purposes of this tutorial we will discuss how to move a...plasmids. Because you lose some DNA during the gel purification step, it is important to digest plenty of starting...

... DNA fragments of interest, such as genes. Figure 1: Creation of recombinant DNA. Working with Plasmids...U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA EPI300 LGC Biosearch Technologies...Addgene; Promega e14-(McrA-) recA1 endA1 gyrA96 thi-1 hsdR17(rK- mK+) supE44 relA1 Δ(lac- proAB) [F traΔ36...galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ- leu mtl-1 Top10 Invitrogen F- mcrA Δ(mrr-hsdRMS-mcrBC) Phi80lacZM15...Chemistry techniques enable the isolation and purification of cellular components, such as DNA, but in ...1,000–20,000 base pairs) for physical isolation (purification) and manipulation. With current cloning technology...stable — Plasmids are stable long-term, either as purified DNA or within bacterial cells that have been preserved...