We narrowed to 132 results for: Pts

...break repair, if non-homologous end joining (NHEJ) disrupts the gRNA target site after the first HDR event...frames downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002... mRNA Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–...

...for a Group Leader position, key first author manuscripts need to be ready to write by Year 3.5. Thus, ...model?  PFs need to be regularly thinking about manuscripts. The other items will follow. If you get more...thereby help decrease the time to your first manuscripts. 4. Balance focus and exploration Researchers...

... be a few tips for scientific communication (manuscripts, talks, etc…!)  Deep Dives You probably are familiar...series, which introduces common techniques and concepts. But what if you’re looking for information beyond...

... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project...

...parallel and provide serotype data in minutes and the scripts  can be easily customized to detect other sequences...are excited to share the procedures and Python scripts with laboratories and viral vector cores routinely...

...created a video to help scientists visualize the concepts discussed in the paper. The transcriptional control...behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors often fall...

...round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent...

...sciences were called into question after credible attempts to replicate major findings could not be replicated...difficult to replicate. Unclear methods Until someone attempts to replicate the results of another’s work, it...

... protein (orange). A low imidazole wash buffer disrupts the weak interaction of the contaminating protein...protein of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest and...

...the temporal and spatial localization of mRNA transcripts in tissue. This is useful for labeling specific...results in an observable “sidewinder” phenotype that disrupts wildtype locomotion.   Create your own plasmids...

...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As...

...first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology...

...carried by extracellular vesicles (EVs), edited transcripts could endow neighboring cells with pro-oncogenic...system of dynamic and redundant roles. This makes attempts to purify or overexpress proteins inappropriate...

...therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility for preclinical...similarly low rates of reproducibility. Failed attempts to apply irreproducible results to future work...

... (Fig. 1). They first copy themselves as RNA transcripts and then rely on a reverse transcriptase enzyme...mechanism. First they copy themselves as RNA transcripts and then use their reverse transcriptase (RT)...

... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding...

...RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to represent the primary...variant in 38 types of human cancer. Many of the transcripts are not available from other existing sources...

... on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question...

...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...

...Any (see notes) Any Data repository Only accepts data for antibodies in Addgene’s repository  ...