We narrowed to 129 results for: Pts
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TypeBlog Post...be published, this can delay publication and manuscripts may go through several rounds of revision before...and doesn’t help your chances of getting your manuscripts published in the future. Disregard reviewer’s...responsible for assessing the scientific content of manuscripts submitted for publication. Holds a PhD in Cell...
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Plasmid Tools for Microbiome Studies
TypeBlog Post..., the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit of this...the organisms present while sequencing of all transcripts gives clues into what the microbes are doing.... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...break repair, if non-homologous end joining (NHEJ) disrupts the gRNA target site after the first HDR event...frames downstream of the gRNA target site so that transcripts undergo nonstop decay (Frischmeyer et al., 2002... mRNA Surveillance Mechanism That Eliminates Transcripts Lacking Termination Codons. Science 295:2258–... -
9 tips for a successful postdoctoral experience
TypeBlog Post...for a Group Leader position, key first author manuscripts need to be ready to write by Year 3.5. Thus, ...model? PFs need to be regularly thinking about manuscripts. The other items will follow. If you get more...thereby help decrease the time to your first manuscripts. 4. Balance focus and exploration Researchers... -
Summer Fun at Addgene!
TypeBlog Post... be a few tips for scientific communication (manuscripts, talks, etc…!) Deep Dives You probably are familiar...series, which introduces common techniques and concepts. But what if you’re looking for information beyond... -
Fostering communication in remote collaborations
TypeBlog Post... that everyone can access data and drafts of manuscripts and grant applications in real-time so they can...figure out are timelines for experiments and manuscripts, who is responsible for which parts of the project... -
Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation
TypeBlog Post...parallel and provide serotype data in minutes and the scripts can be easily customized to detect other sequences...are excited to share the procedures and Python scripts with laboratories and viral vector cores routinely... -
Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]
TypeBlog Post...created a video to help scientists visualize the concepts discussed in the paper. The transcriptional control...behaviors, such as differentiation or metabolism. Our attempts to manipulate these cellular behaviors often fall... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post...round of translation While ribosome profiling co-opts the physical location of the ribosome, TRICK (Translating...cell cycle protein Emi1, showing that ~80% of transcripts with a long 5’ UTR were translationally silent... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...sciences were called into question after credible attempts to replicate major findings could not be replicated...difficult to replicate. Unclear methods Until someone attempts to replicate the results of another’s work, it... -
To Each HIS Own
TypeBlog Post... protein (orange). A low imidazole wash buffer disrupts the weak interaction of the contaminating protein...protein of interest. A high imidazole elution buffer disrupts the interaction of the protein of interest and... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...the temporal and spatial localization of mRNA transcripts in tissue. This is useful for labeling specific...results in an observable “sidewinder” phenotype that disrupts wildtype locomotion. Create your own plasmids... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...'ve overlooked regulatory fragments of these transcripts. I remember when small RNA was just something...circRNA datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...first site accepts the sgRNA target sequence for U6 promoter expression and the second site accepts the homology... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...therapeutics, and sometimes formulating new biological concepts. However, rates of reproducibility for preclinical...similarly low rates of reproducibility. Failed attempts to apply irreproducible results to future work... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... (Fig. 1). They first copy themselves as RNA transcripts and then rely on a reverse transcriptase enzyme...mechanism. First they copy themselves as RNA transcripts and then use their reverse transcriptase (RT)... -
Deep Dive: qPCR
TypeBlog Post... by abundant transcripts, leading to falsely lowered levels for less abundant transcripts. However, adding... -
27 Hot Plasmids from 2016
TypeBlog Post...RAS signaling pathway and includes 180 unique transcripts. Each gene was chosen to represent the primary...variant in 38 types of human cancer. Many of the transcripts are not available from other existing sources... -
Antibodies 101 Series on TikTok
TypeBlog Post... on TikTok for bite-sized portions of antibody concepts. Each short video answers an Antibodies 101 question... -
A Case Study of Addgene Bioinformatics
TypeBlog Post...of our Data Team, “Our patchwork collection of scripts evolved into a single, organized pipeline and accelerated...