We narrowed to 53 results for: RIK

1. Reagent Repositories Are Speeding up Science During the Pandemic

   Type

   Blog Post

   ...requested dozens of times in 2 weeks. This is a striking example of the contributions that scientists are...

2. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

   Type

   Blog Post

   ...the mouse CRISPR knockout library at Addgene. Henriksson et al., Cell, 2019. PubMed PMID: 30639098. LipoGlo...

3. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   ...high throughput platforms, Addgene and Ginkgo are striking a balance by modifying certain processes in both...

4. Human Germline Editing Using CRISPR

   Type

   Blog Post

   ...CRISPR editing. Conclusions & implications One striking feature of this paper is the failure of the embryos...

5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   ...on the dimerization tendency in living cells. Strikingly, the OSER assay reveals that tagRFP does not ...

6. All in a Twist: dsRNA

   Type

   Blog Post

   ... to genome instability. Thus, it’s important to strike a balance between productive levels of R-loops ...

7. Open Resources and Plasmid Tools For Studying C. elegans

   Type

   Blog Post

   ...Central PMCID: PMC2238901. Schwartz, Matthew L., and Erik M. Jorgensen. "SapTrap, a toolkit for high-throughput...

8. Progress Towards a PAM-Free CRISPR

   Type

   Blog Post

   ...although both are of course preferred! When trying to strike that balance, you may wonder: how close is close...

9. Adapting Toehold Switches to Detect Zika Virus

   Type

   Blog Post

   ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

10. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    ...with very little money. So when did inspiration strike us so hard that we upended our lives and took this...

11. Fluorescent Protein Guide: Subcellular Localization

    Type

    Collection

    ...signal moxGFP Erik Snapp 68126 ERoxBFP Endoplasmic Reticulum ER retention signal oxBFP Erik Snapp 49150 ...apparatus GalT signal anchor transmembrane domain oxBFP Erik Snapp 158005 pCMV-mGold-MannII-N-10 Golgi apparatus...

12. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type

    Blog Post

    ... all possible consequences of FP aggregation, as Erik Snapp’s blog post shows. To avoid these issues, ...

13. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    ...that UV light only kills microorganisms that it strikes directly. UV light exposure is also harmful for...

14. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    ...The ocean is full of striking visuals from fluorescent jellyfish, bioluminescent phytoplankton, and colorful...

15. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as a tool for titration...

16. Plasmids 101: Stringent Regulation of Replication

    Type

    Blog Post

    ...DNA replication and regulation Plasmids must also strike a balance: replicating too slowly will eventually...

17. 10 Steps to a Perfect Science Talk

    Type

    Blog Post

    ... right now to capture interesting key results, striking images, creative ideas, and engaging stories as...

18. Chemogenetics AAV Preps

    Type

    Collection

    ... Inhibition mCherry fusion Flp-dependent 8, rg* Ulrik Gether 154868 pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA... Activation mCherry fusion Flp-dependent 8, rg* Ulrik Gether 119741 AAV SYN flex PSAM4 GlyR IRES EGFP ...

19. Plasmids 101: Repressible Promoters

    Type

    Blog Post

    ...PMCID: PMC393301. Da Silva, Nancy A. & Sneha Srikrishnan. “Introduction and expression of genes for metabolic...

20. Worm Expression Resources

    Type

    Collection

    ...Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection of plasmids that streamlines...Mos1 Based Transgenesis miniMos Transgenesis Kit - Erik Jorgensen Lab. This kit contains 29 plasmids used...