We narrowed to 45 results for: RNAi
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TypeCollection...Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression Stem Cells Synthetic...
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Five Popular Model Organisms
TypeBlog Post...be easily knocked-down through RNA interference (RNAi) by feeding the worm bacteria expressing specific...of interest (Fire et al., 1998). The discovery of RNAi in the worm actually rewarded depositors Andrew ... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...started with their labs studying the mechanisms of RNAi, the field for which Craig Mello and Andrew Fire... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog Post...hypersaline samples, were engineered and adapted for RNAi experiments for mammalian cell lines. The Turner... -
Hot Plasmids - January 2023
TypeBlog Post...RNA targeting, with a leg up on the alternatives - RNAi and Cas13. CRISPR-Csm doesn’t degrade proximal RNAs... -
15 Years of Addgene: The Top 15 Plasmids
TypeBlog Post...shRNA is used as a negative control for mammalian RNAi experiments. Find the scrambled shRNA vector. pLKO... -
Plasmids 101: The protein expression toolbox
TypeBlog Post...proteins (when knockouts aren’t viable). siRNA or RNAi are small, double stranded RNA molecules that degrade... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol... using the pLKO.1 vector, a backbone used by the RNAi consortium for targeting human and mouse genes. ... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post... & CRUSH – rapid & conditional gene silencing in RNAi transgenic mice The Jackson-Grusby lab has designed... in conditional and reversible gene silencing in RNAi transgenic mouse models and embryonic stem (ES) ...transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which Cre expression causes... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...Listen to the TRIM-Away podcast segment CRISPR and RNAi have helped researchers alter DNA sequence and RNA... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog Post...bacterial expression, lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...translation either by blocking initiation or splicing. (RNAi, in contrast, engages existing gene regulatory mechanisms... -
Plasmids 101: Control Plasmids
TypeBlog Post...targeting or genome editing technologies, such as RNAi or CRISPR, non-targeting controls may be appropriate... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...It’s possible to actually silence this gene with RNAi to make a polygamous species monogamous. Anolis ... -
Antibodies 101: Validation
TypeBlog Post...expected protein. Common approaches include using RNAi to knock-down expression of your target or CRISPR... -
Important Considerations When Using AAVs
TypeBlog Post... Kay. "Future of rAAV gene therapy: Platform for RNAi, Gene Editing and Beyond." Human Gene Therapy ja... -
Tetracycline Inducible Expression
TypeCollection...Elledge 111177 LT3GEPIR Lentiviral Tet-On inducible RNAi with miR-E (miR-30 variant)-based backbone rtTA-... -
CRISPR History and Development for Genome Engineering
TypeCollection...skyrocketing popularity of CRISPR echoing that of RNAi and iPS cells. Genome engineers continue to work... -
Lentiviral Vector Guide
TypeGuide...). Lentivirus-delivered stable gene silencing by RNAi in primary cells. RNA , 9 (4), 493–501. https://... -
Delivery Methods for Generating iPSCs
TypeBlog Post... gene-specific plasmids (including reporters and RNAi) for: NANOG OCT4 SOX2 MYC KLF4 LIN28 Check ...