Protocol - pLKO.1 – TRC Cloning Vector
Type
Protocol
...resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu...www.broad.mit.edu/genome_bio/trc/rnai.html Background on RNAi mechanism: RNAi animation Whitehead siRNA Selection...Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has...the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes. ...of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids... For more information, visit Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited... For more information, visit Addgene’s Mammalian RNAi Tools page. E.2 Protocol for Producing Lentiviral...