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We narrowed to 45 results for: RNAi

Showing: 1 - 20 of 45 results

  1. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...: creation of the RNAi trigger delivering the RNAi trigger characterizing the RNAi phenotype. Although... 1: Schematic of an RNAi-inducing transgene. Typically, the desired target of RNAi is expressed as inverted...might incorrectly relegate RNAi to history. The straightforwardness of RNAi is unmatched—unlike CRISPR... gene of interest. Tools for RNAi Arguably the greatest advantage of RNAi is the straightforward nature...gene or genes of interest to trigger RNAi activation. A typical RNAi experiment can be divided into three...present at the first stage of an RNAi-based investigation. Creation of the RNAi trigger requires a few initial...generate a long-term RNAi phenotype. Long hairpin RNA The easiest approach to induce RNAi involves the expression...

  2. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    ...Cecchetelli  Listen to the C. elegans RNAi podcast segment RNA interference (RNAi) is extensively used in C. elegans...gene being targeted via RNAi. Recently the Vellai lab has created an improved RNAi vector that leads to ... standard RNAi protocols without the need for mutant genetic backgrounds. The standard RNAi vector, L4440... the hot plasmids episode! New, highly efficient RNAi construct for C. elegans gene knockdown Article ...elegans research to study gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA...experiments are to physically set up in the lab, RNAi is often completely ineffective or results in low...are genetically altered to overexpress endogenous RNAi pathways. These mutations however can interfere ...

  3. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    ...research career working on miRNAs and RNAi. He first heard about RNAi at his MIT interview during a lecture..., “what I loved about [RNAi], ... it was just this complete black box. The RNAi process had just been ...helps researchers in a variety of fields develop RNAi screens to answer their own research questions. ...Institute For his post-doc, Doench worked on using RNAi screens to learn more about cancer biology in Ed...Doench starting working with David Root in the then RNAi Platform, which has since evolved into the Genetic...background in history and specifically the history of RNAi left him a bit skeptical of the technology at the...explain that, “everything that could be done with RNAi in terms of determining what genes are involved ...

  4. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...Evaluating Gene function by RNA interference (RNAi) RNA interference (RNAi) is a powerful tool for performing targeted...-stranded RNA (dsRNA)-mediated RNA interference (RNAi) to knockdown expression of specific gene expression...for both in situ hybridization and dsRNA-induced RNAi experiments. Notably, this plasmid harbors a multi-cloning...plasmids and protocols required for both WISH and RNAi experiments, as well as how to perform worm amputations...expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts or “dices” dsRNA into...feed the mixture to the worms. The phenotypes of RNAi knockdown treatment should be apparent after three... Find plasmids for Mammalian RNAi ...

  5. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    ...interference (RNAi) is, as the name might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark...method for many labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie... quickly rose to popularity. Compared to CRISPR, RNAi methods are often quicker and the repositories of...popular choices for gene silencing. RNA interference RNAi is a useful tool to investigate the roles of specific...via transfection or viral delivery of a plasmid. RNAi is an umbrella term for a number of different applications...end_module_block %}   Interfering in the future While RNAi has retreated a bit to the background, it remains...validated shRNA sequences for your intended target. RNAi is a valuable tool for your research arsenal, and...

  6. Mammalian RNAi Tools

    Type
    Collection
    ...Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources RNA interference (RNAi) is an RNA-mediated...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...Ago2), which facilitates cleavage of the target. RNAi using siRNA follows a similar mechanism. Since it...are available. To find plasmids containing other RNAi components, like argonaute, please search our full...

  7. All in a Twist: dsRNA

    Type
    Blog Post
    ...also known as RNAi, are short, dsRNA molecules designed to degrade mRNA transcripts. RNAi activates the...compatible sequence is found (typically RNA that the RNAi is designed against), RISC can repress translation...lab to deplete expression of proteins of interest. RNAi has even made it into agriculture as species-specific...immune responses are also a concern when it comes to RNAi therapeutic delivery. Thus, balancing the amount...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    ...Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated gene...Scientists can express siRNAs to direct the cellular RNAi machinery to silence target genes. This empty MuLV...cells.  Learn more about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201...Plasmid #10878) This plasmid has been used by The RNAi Consortium, also known as the Genetic Perturbation...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC): www.broad.mit.edu...www.broad.mit.edu/genome_bio/trc/rnai.html Background on RNAi mechanism: RNAi animation Whitehead siRNA Selection...Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone upon which The RNAi Consortium has...the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes. ...of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids... For more information, visit Addgene’s Mammalian RNAi Tools page. Several other laboratories have deposited... For more information, visit Addgene’s Mammalian RNAi Tools page. E.2 Protocol for Producing Lentiviral...

  10. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...there are other options which are worth considering. RNAi screens are similar to CRISPR, but instead of knocking...genome), they knock down gene expression temporarily. RNAi libraries are especially useful if you are interested...to check out our CRISPR library resources or our RNAi resources and go fishing! References and resources...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...real challenge to troubleshoot RNAi failures. Potential explanations for RNAi not reducing the antibody signal... of the RNAi Correctly interpreting your data therefore necessitates careful design of RNAi sequences...feasible, an alternative approach is RNA interference (RNAi). The most common method is transfection of short...

  12. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    ...These screens provide an alternative to genome-wide RNAi screens, which although highly effective, are affected...non-specific toxicity, and off-target effects. The flaws of RNAi screens are well characterized and strategies exist...CRISPR screens are prone to some of the same flaws as RNAi screens, such as non-specific toxicity due to both...

  13. Worm Expression Resources

    Type
    Collection
    ... for use in worm research. This includes CRISPR, RNAi, empty backbones, fluorescent proteins, and Cre ...Silencing Genomes - Cold Spring Harbor's guide to RNAi in C. elegans WormAtlas - A database featuring behavioral...

  14. Plant Plasmids and Resources

    Type
    Collection
    ...Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent Proteins Other Resources...expression or plant transformation - Silin Zhong. RNAi The following collections are designed for gene ...

  18. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...with very low basal activity shRNA/RNAi Gene silencing Browse RNAi empty vectors for cloning in shRNAs...Collection of plasmids from the Fire Lab L4440 - RNAi in C. elegans Also see our Worm Plasmids...
Showing: 1 - 20 of 45 results