We narrowed to 44 results for: RPA;

1. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...genes of some human cancers from their normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/...

2. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   ...Schizosaccharomyces pombe, have the same function as their counterparts in S. cerevisiae. The two yeasts are thought...

3. Cpf1 Update: Comparison to Cas9 and NgAgo

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   ...its guide RNAs are smaller than their SpCas9 counterparts, so they will be easier to deliver in low-capacity...

4. Common Injection Routes in Mice

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   ...instillation is very similar to its intratracheal counterpart, as both procedures target the lungs. The main...

5. Viral Vectors 101: Voltage Indicators

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   ...have kinetics comparable to its one component counterpart and is significantly brighter and available with...

6. PITChing MMEJ as an Alternative Route for Gene Editing

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   ... see the linked blog posts. Compared to its counterparts NHEJ and HDR, MMEJ doesn’t get a lot of press...

7. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

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   ...genomic target sites.  This obstacle was recently surpassed with the discovery of CRISPR-transposons by the...

8. Plasmids 101: Choosing an Antibiotic Resistance Gene

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   Blog Post

   ...ve got amp? Well, amp is less stable than its counterparts and is also known to produce satellite colonies...

9. Viral Vectors 101: The Retroviral Lifecycle

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   ...mechanisms, only one of the two strands’ DNA counterparts from step 2 will be integrated by the viral ...

10. CRISPR 101: Making a Knock-In Cell Line

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    ...more planning and finesse then their knock-out counterparts. To kick off a knock-in experiment the first...

11. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...its guide RNAs are smaller than their SpCas9 counterparts, they will also be easier to deliver in low-...

12. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...mirrors the complexity of understanding their wild counterparts. Those familiar with viral vectors, and especially...

13. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post

    ...revealing that about 70% of human genes have a counterpart in zebrafish. Understanding the function of these...

14. Fluorescent Proteins 101: Aptamer Fluorophores

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    Blog Post

    ...important advantages over their amino acid-based counterparts, antibodies and enzymes, such as: Design and...

15. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...has much lower efficiency than its wild-type counterpart.  In search of a smaller, but equally potent ...

16. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    ...depleted at greater rates than their non-targeting counterparts, suggesting that safe-targeting guides are more...

17. Five Popular Model Organisms, Part 2

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    ...that nearly all disease-linked human genes have counterparts in the rat (Gibbs, et al., 2004). Additionally...

18. Five Popular Model Organisms

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    Blog Post

    ...chromosomes and about 23% of yeast genes have a counterpart in humans (Liu et al., 2017) . Thus yeast can...

19. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...generally more toxic to cells than their plasmid counterparts. Another consideration for dsDNA templates is...

20. Hot Plasmids: Spring 2025

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    Blog Post

    ...Proximity ligation tools like TurboID and its mini counterpart label nearby proteins with biotin. The biotinylated...