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We narrowed to 181 results for: SPEN;

Showing: 21 - 40 of 181 results

  1. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...subset of cells in a complex population. Have you spent hours in the biosafety cabinet with cloning rings...system An electronics system  Cells are first resuspended in a pressurized buffer called sheath fluid and...instrument clogs, ensure that samples are single cell suspensions. For cells that tend to aggregate, pass the sample...

  2. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...that scientists have amazing stories to share. I spend a lot of time telling scientists about Addgene’s...how to deposit and request plasmids, but I also spend a lot of time listening. I’ve had hundreds of conversations...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...biorender.com   Both gradients can be informative, so spend some time thinking about which type of gradient ... your blot’s data, the more time you’ll want to spend optimizing it. Blots for quantification generally...antibodies or validate them yourself, so you don’t spend time troubleshooting an assay that’s fine with antibodies...

  6. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...GFP were published in the 1990s, biologists have spent a lot of time making things glow. Chances are you...spontaneous conversion did not occur. Tobacco suspension cell culture with cytosol-targeted GFP. White...

  7. Science as Art

    Type
    Blog Post
    ...www.microworldsphotography.com/. Another artist who spent his life blurring the lines between science and ...Cambridge and Bristol. If you’re a scientist who spends many hours at a microscope, you too can become ...

  8. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ... plasmids, so that you can spend less time researching the basics and spend more time developing cleverly...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...scientists accomplish their goals. Many nonprofits must spend a lot of resources continuously raising money from...self-sustaining. With this unique model, we are able to spend resources on maintaining quality and starting new...

  10. Advice for Choosing a Research Project

    Type
    Blog Post
    ... into your interests and will determine how you spend a good chuck of your mental energy and time. The...At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

  11. What the HEK?

    Type
    Blog Post
    ...though derivatives have been adapted for growth in suspension. Of the many derivatives of HEK-293, the most...systems, such as yeast. HEKs can also grow in suspension, which allows for large scalability, and can ...

  12. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...letters after your name (P-H-D).  Finally, having spent your entire career in science, you are likely lacking...entrepreneur at 22. Start with your network You've spent your life making connections and finding mentors...

  13. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ... I was having outside of the “ivory tower.” I’d spend the afternoon lovingly tending to the stem cells...cells I was using in an experiment, then spend the evening listen to some bloke at the pub telling me that...

  16. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ... makers to keep things legible. It is faster to spend more prep time on an experiment than it is to rerun...when I was tired, stressed, or rushing. I would spend the time quietly running through the experiment ...

  17. RNA Extraction Without A Kit

    Type
    Blog Post
    ...least 4 hours.   If your final RNA sample(s) are resuspended in water or TE buffer, store them in a -80°C ...products on the market to choose from) on your resuspended RNA sample will help ensure DNA contamination...

  18. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ... the Scientific Support team, much of my day is spent helping to resolve problems, but even so, I wasn...framework to the myriad challenges we encounter. As you spend time in a lab learning techniques and taking on ...

  19. Tips for Technical Support Calls

    Type
    Blog Post
    ... years as a senior scientist at Addgene. Having spent that long in customer service, I've daydreamed about...utility in the least amount of time. Though I now spend far less time answering help emails and phone calls...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...ultracentrifugation of the viral stock, followed by resuspension of the collected particles in a smaller volume...white pellet. The pellet can then be rinsed and resuspended in cold sterile PBS.  Alternatively, to concentrate...
Showing: 21 - 40 of 181 results