We narrowed to 32 results for: SaCas9

1. Cpf1: A New Tool for CRISPR Genome Editing

   Type

   Blog Post

   ...that of the widely used SpCas9 and comparable to SaCas9. As seen with Cas9 orthologs, Cpf1 efficiency varies...

2. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   ...most widely-used Cas enzyme, the development of SaCas9, NmeCas9, Cas12a enzymes, and engineered variants...

3. Treating Muscular Dystrophy with CRISPR Gene Editing

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   Blog Post

   ... et al. and Tabebordbar et al. used the shorter SaCas9 with AAV8 and AAV9, respectively. Although each...

4. Plan Your Experiment

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   Collection

   ... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...

5. CRISPR History and Development for Genome Engineering

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   Collection

   ...also been characterized. Staphylococcus aureus (SaCas9) is smaller than SpCas9 and more easily packaged...

6. CRISPR Guide

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   Collection

   ...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

7. Adeno-associated virus (AAV) Guide

   Type

   Guide

   ...has been to use smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab...lab has developed plasmids containing SaCas9 optimized for their use in rAAV vectors . Read our blog post...

8. Plan Your Experiment

   Type

   Guide

   ... Cas enzymes) Transient or stable expression of SaCas9 and/or gRNA Infects dividing and non-dividing cells...

9. CRISPR Guide

   Type

   Guide

   ...enzyme, additional Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence...meningitidis ), which are about the same size as SaCas9, with higher fidelity than SpCas9. FrCas9 (from...GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus sp. (AsCpf1...

10. Multiplex Genome Editing with CRISPR-Cpf1

    Type

    Blog Post

    ... crRNACpf1 Endonuclease size spCas9: ~4 kbsaCas9: ~3 kb ~3.9kb crRNA/gRNA length gRNA: ~100 ...

11. 27 Hot Plasmids from 2016

    Type

    Blog Post

    ... of dCas9-VPR plasmids, which already includes dSaCas9-VPR in an AAV transfer vector for in-vivo expression...

12. Neurodegeneration Plasmid Collection

    Type

    Collection

    ...Episodic ataxia Xiaolong Wang 159912 pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3 HTT CMV Huntington's Larry Zweifel 160435...