...desired concentration. If the sample concentration is still too low, repeat the centrifugation until the volume...

...the mandate has been removed, institutions should still go ahead with raises, as for years there have been...planning to raise salaries prior to the injunction are still honoring these plans. We are now collecting data...following the injunction, 59% of all postdocs are still expected to receive salary raises - down from the...to get a salary increase prior to the injunction still won’t after the injunction (see below). Of the ...salaries, the only postdocs we knew for certain to still be getting their raise were: postdocs at institutions...University of Louisville in Kentucky, the raises are still going ahead as planned. As we are receiving data...

...saving the researcher both time and resources (3). Still, researchers generating transgenic animals may choose...helped researchers at a time when sequencing was still in the early days of development. Today, researchers...restriction enzymes are certainly not dead yet! Labs still keep restriction enzymes on hand for cloning and...sequencing (more on that below), restriction mapping may still be used to screen multiple colonies before selecting...expensive sequencing fuels a sequencing revolution that still prevails. Sequencing whole genomes or profiling ...techniques have largely replaced these, these techniques still have useful applications and have informed many ...

... as “instillation” or “gavage,” as they do not require any punctures. Intratracheal instillation Intratracheal...Skill required: advanced Intranasal instillation Intranasal instillation is very similar to its intratracheal...Intratracheal instillation is used to deliver substances directly to the lungs. It is an advanced technique...

...after graduate school, at least not while you’re still in the thick of it. About three or four years into...ways to get a taste of a variety of careers while still working towards your degree. For me, experimenting... said, “Boy, I love science,” and I realized, I still do too! Beyond the outreach experience I gained ...back into lab the next day and remembered why I still love science. Keep building on your time in graduate...look back at a positive, fulfilling experience. I still use my network and graduate school resources daily...

...behind at the site of excision, this approach is still not considered safe for therapeutic applications...screening cells for the presence of transgenes is still an important control. Additionally, Sendai virus...The typical drawbacks of using transfection will still exist: transfection efficiency is cell-type dependent...by dilution with each cell division, but it can still take several passages for the minicircle to be completely... that ⅔ of subclones generated from EBNA1 iPSCs still had episome DNA present. DNA-Free Methods RNA...reprogramming proteins for 8 hours a week for 6 weeks, and still required further culturing to generate iPSC colonies...

...nucleotide transitions but still requires a nearby PAM, although these are still early days for this technology...gRNAs to 5 – 65% of the protein coding region will still result in many dozens of gRNAs to choose from. With... While SpCas9, with a PAM preference of NGG, is still the most widely-used Cas enzyme, the development...basic landscape of mismatches that can nevertheless still lead to activity has been established, and can be...

...membrane pores) transfection. These techniques are still used and efficiently transfer plasmid DNA efficiently...vector. Lentiviral vectors Although retroviruses are still in use, considerable efforts have been devoted to...However, in this vector production system, there is still a potential risk for the generation of replication...however improving the safety of these vectors is still an active area of research due to the possibility...Replication-incompetent lentiviruses with human tropism can still infect the researcher and insert into her/his genome...

...application, and the efficacy varies widely — it still almost always results in higher ratios of desired...cost of improved efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for...obtain PEs small enough to deliver with AAVs but still efficient at long and complex edits. The team also...this tail is degraded, the truncated pegRNA can still guide Cas9 and compete with full pegRNAs for access...engineered virus-like particles. However, there’s still a long way to go before we see prime editors in ...

...extraction methods evolved into a simple protocol still used today There are many alternative methods for...-phenol-chloroform. In this method, samples are still homogenized and lysed in a guanidinium thiocyanate...hours, and variations on this no-kit method are still widely used today (Chomcynski and Sacchi, 2006)....Making the simple protocol even more foolproof (still without a kit!) As mentioned above, working with...

...developed to break through the resolution barrier while still utilizing fluorescence microscopy, is another option... between them to ensure the expanded images are still a good representation of reality. Third, expansion...secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity can appear reduced...https://doi.org/10.1098/rsta.2021.0110  Sherry DM, Stiles MA (2022) Improved fluorescent signal in expansion...

... plasmid is delivered to your target cells, you still need to select for the cells that contain it. Antibiotic... approach in a mammalian cell experiment should still include an antibiotic resistance gene for growth...express your protein of interest. However, you do still need a species-compatible promoter for expressing...between prokaryotes and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak...

..., there still remains the problem of prevention. Asbestos exists pretty much everywhere, still, in old...mesothelioma are creeping upwards, and while most people still die within a year of diagnosis, the hope is that...United States and many other countries, and it is still imported, exported, and used throughout the world...

... too many moving parts in the whole process to distill a one-size-fits-all recipe for success. What I ...the Dean, and even some people in your field that still need to understand your specific questions and experiments...directly state your official aims, but I suggest distilling them into simple goals/outcomes. This isn't the...show interest in other faculty members’ research. Still a Grad Student? Get Tips on Getting the Most out...

...be visualized by running your PCR on a gel. It’s still paramount that you sequence clones to verify that...homozygous clones, however, sequence confirmation is still needed to verify the correct sequence was inserted...it requires a little extra wet lab work, this is still a great option, especially if the options outlined...data analysis. If NGS is not an option, you can still detect off-target effects with Sanger sequencing...

...the office had partially reopened. But there were still many restrictions in place, most importantly, masks...would allow me to set up a camera in a room, but still have control over focus, zoom, stop/start record... to mount a battery to it if necessary. I could still control the camera from a distance if I needed to...in-lab filming and remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth...

...expression, low levels of spurious expression were still observed in many systems. So what is the cause of...in order to minimize off-target expression while still achieving adequate expression in your cells of interest...recombination rate (0.1% and 0.13% respectively) still required a 200-300 fold dilution to minimize off-target...accuracy and reproducibility of the data and can instill greater confidence when interpreting the outcome...

...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different...produce SuperFi-Cas9, a high-fidelity variant that still retains high cleavage efficiency (Bravo et al., ...et al., 2014). New discoveries on this topic are still being made! Conclusion Finally, we can summarize...

...neuroengineering, and some of the optogenetic tools that are still missing. Optogenetic tools for neuroengineering ...focus on the next round. Addgene: What tools are still missing from the optogenetics toolbox? Boyden: That's...question. We've made a lot of progress. I would say we still would like to find ways to make these tools more...

... That papers with plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations...step. There are lots of other factors that could still be controlled for. As an example, plasmid-related...of correlation. But even with that caveat, it is still suggestive that we see such a big difference in ...