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We narrowed to 889 results for: TRI

Showing: 21 - 40 of 889 results

  1. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...of how to analyze a restriction digest:     Restriction digest tips and tricks: The following tips ...plasmids. Here, we'll cover restriction digest analysis. Diagnostic restriction digest Diagnostic digests...Diagnostic restriction digests are comprised of 2 separate steps: 1) incubating your DNA with restriction enzymes...to confirm the sequence of all the plasmids we distribute. This method is time-intensive, so we recommend...organization of different features within the plasmid. Restriction analysis can also be used successfully even if...relative sizes of the resulting DNA fragments. Restriction digests are commonly used to confirm the presence...backbone. To do this, you'll use enzymes with restriction sites that flank the insert. You will need to...

  2. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type
    Blog Post
    ...providing plasmids to researchers in developing countries. We are proud to introduce the 5 scientists who...molecular biotechnology for improving expression of nutritionally and therapeutically significant proteins. In...Encoded H2O2 Probe with Improved Performance for Ratiometric and Fluorescence Lifetime Imaging” ACS Chemical...Expression Systems Learn about Global Plasmid Distribution ...

  3. Technical Design of a Western Blot

    Type
    Blog Post
    ...alteration) Expensive buffers Tris-tricine 2.5–40 kDa Tris-tricine 30 V, 1 hour or 100 V, 1–2 hours...Limited protein size range Tris-acetate 40–500 kDa Tris-tricine 150 V, 1–3 hours Good for ...Running conditions Pros Cons Tris-glycine 6–400 kDa Tris-glycine 100 V, 1–2 hours Easy and...that Bis and Tris gels run proteins in different patterns, due to their differing chemistries. You therefore...-life; can alter proteins due to high pH Bis-tris 6–400 kDa MES for proteins <40 kDa 180 V, ... protein alteration) Expensive buffers Bis-tris 6–400 kDa MOPS for proteins > 30 kDA 200 V...therefore cannot compare Bis and Tris gels or blots to each other. Once you know your gel type, you’ll need...

  4. Genetic Code Expansion

    Type
    Collection
    ... Plant TAG Chang Liu 85484 pDule-tfmF A65V S158A tri-fluoromethyl-phenylalanine synthetase M. jannaschii... Mehl 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial...Huber 177311 pRSF-G1TFAKRS M. archaeon N6-(trifluoroacetyl)-L-lysine (TFA-Lys) Bacterial TAG Thomas Huber...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 201922...

  5. Addgene’s Newest Way to Speed Your Research: The Cloning Grade DNA Service

    Type
    Blog Post
    ...project We will ship you  2 µg of DNA in 20 µl of Tris buffer suitable for molecular biology experiments...this period, we’d love to hear from you if you’ve tried out this service! Let us know what you think. You...excited to begin offering this service to further contribute to our mission to “accelerate research and discovery...

  6. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...complex and dynamic field. We’re going to focus on a tried and true method of finding enhancers by isolating...mono-methylated and acetylated histones, but not tri-methylated histones, functions as an enhancer. We... me1, me3, and ac – denoting mono-methylation, tri-methylation, and aceylation, respectively (these ...suppose you have a piece string that’s 10 meters long. You twist and roll the string into a tight ball. Now...This post was contributed by guest blogger, Aneesh Karve, CTO at Quilt Data. This post was originally...follows: (mono_methylation + acetylation) - tri_methylation In the next section we’ll apply the above formula...Now the two ends of the string, instead of being 10 meters apart, are mere millimeters apart. Similarly...

  7. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... an exciting and emotional time!” George Church tries to recruit people who are nice as top criterion ...latest results as soon as the gels ran.” Dr. Ellis tries to lead his team as a scientists and a friend. George...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...and potent liver transduction. While we have not tried it, it might be worth investigating for both Q6 ...cross-react with each other. Multiple labs have tried to transfer PHP.eB's enhanced CNS tropism to other...understanding that AAV-BR1 has the same genome size restrictions as other AAV. Therefore, one should aim to keep...

  9. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...end up stressed and rushing, no matter how hard I tried to build in extra time before the experiment. What...any experiment. That, more than anything else I tried, made a huge difference in my ability to focus, ...used Roberson Lab, UPenn. Western blotting. Retrieved Feb. 7, 2022. https://www.med.upenn.edu/robertsonlab...

  10. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...modular code. Use tried-and-true tools We use Python, Django, Apache, and MariaDB. Using tried-and-true tools...tracked. Terminology and language are tricky Names for things will trip everyone up at some point, decide ...messiness of biology. These examples are intentionally trivial, but they're here to help tell this story. In this...plasmid and now it’s time for our lab technicians to retrieve the sample and get it ready for shipping. In the...contents might leave you sad and confused when a tricky procedure failed and one of your techs started ...

  12. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...plasmids for transfer to recipient cells during a tri-parental mating scheme. Mobile-CRISPRi integrates...transferred to a recipient bacteria strain of interest by tri-parental mating. Donor bacteria contain a chromosomal...presence of trimethoprim, cultures treated with a folA gRNA were more sensitive to trimethoprim when compared... which encodes the target for the antibiotic trimethoprim. When Enterobacter aerogenes, Klebsiella pneumoniae... the folA gene product not been a target for trimethoprim, there would be no changes in sensitivity. Pooled...

  13. Transferable Skills: Negotiation

    Type
    Blog Post
    ...and lead to you being remembered as a person who tries to find solutions that benefit everyone. Even if...negotiation, I will view the “winner” positively if they tried to understand my position and to find a creative...

  14. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    ...have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator ...the technology in an unethical manner, but I also tried not to be an alarmist.  The overarching guideline...and science communicator, and I strive to be a volunteer and contribute to my community. This past year...all of these roles by reaching out to my school district not just as a dad, but as a scientist. When our...it. PTAs have more flexibility than the school district itself in terms of what they do with their discretionary...like this without them! If you live in a school district that has a lot of parents in STEM careers, chances...chances are they’re wondering how they could contribute to the school, too. Though you can recruit parents...

  15. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...month ago. Why haven’t we received our order? I tried searching my PO in the track order portal and nothing... Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the process ...does not own the material in our repository. We distribute it on behalf of depositing institutions. Addgene...

  18. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...is really important. For my Postdoc position, I tried to read at least 50 papers on the topic before I...This guest blog post was contributed by Seán Mac Fhearraigh, PhD, of www.phdadvice.com. I sold out, well...

  19. Interview: Ed Boyden on Optogenetics, Neuroscience, and the Future of Neuroengineering

    Type
    Blog Post
    ...molecules are sensitive to blue light. Many have tried to make green molecules, but then alongside blue...Optogenetics is a toolset that lets you turn the electrical activity of neurons on or off using pulses of...neuron or set of neurons, you can see how it contributes to behavior or pathology, and by disabling neurons...deafness, or other conditions. Addgene: You've contributed various plasmids to Addgene over the years, many...popular. Why has it been so important to you to distribute these tools as freely as possible? Boyden: I ...for different ions. Right now, we can modulate electricity. If you could selectively let in calcium or some...brain. Addgene: Do you have any special tips or tricks for someone looking to try these optogenetic tools...

  20. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... Maguire and colleagues, other researchers have tried to modify it to improve yields. For example, transfecting...for isolating exo-AAVs rely on differential centrifugation steps to separate cell culture supernatant ...
Showing: 21 - 40 of 889 results