We narrowed to 151 results for: Tore;

...high/low) and patterns (tissue/timing) can be monitored under various conditions without necessarily modifying...insert your construct to generate a collection of monitored genes and mutants - viral integration, transposon...

...there was even a time when I was too nervous to ask store clerks for help at the mall. In undergrad I was ... communication skills, I took on TA positions, tutored fifth graders, made a blog and worked as editor-in-chief...

...this sequence. The energy from this breakage is stored as a covalent bond between the cleaved 3′ DNA strand...are planning to transform right away OR you can store the reaction at -20C overnight. 4. Transform TOPO...

...great deal more information that one might like to store about FPs than is currently represented at fpvis...elaborate spectrum analysis tools, and a better way to store and represent within-study photobleaching comparisons...

... abundance of each barcoded clone can then be monitored over time by sequencing the barcodes in the population...receptor (EGFR) with the ClonTracer library and monitored population dynamics after treatment with EGFR ...

...lysate is your primary low titer rAdV-S, and can be stored at -80C for later use or used immediately for amplification.... Purified high-titer rAdV is then dialyzed and stored at -80C. Purification and concentration kits that...

...When this interaction occurs, BirA* activity is restored, and nearby proteins are labeled with biotin. ...

...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

...in a couple of days, some researchers prefer to store freshly harvested virus at 4 °C instead of storing...highly sensitive to freeze-thaw cycles and should be stored in single-use aliquots. Never use a polybrene solution...

...aliquot them into a number of vials, freeze them, and store all but one in liquid nitrogen. The frozen vials...master stock. This ensures that you have many cells stored in their original condition and can replace your...

...approach gives me a bit of flexibility since I can store the cell lysates at -80 °C for up to 6 months before... completed in one long day, or the virus can be stored at 4 °C and buffer exchanged the next day. Refer...

...the restoration of a frame-shift in mCherry that restores fluorescence to monitor APOBEC-Cas-9 mediated ...C-to-T editing by the APOBEC-Cas9 editosome would restore eGFP fluorescence, making it easy to quantify single...

...carried out. While no one is suggesting all data be stored in a retrievable format, we too rarely preserve...issue of proprietary data formats. Many instruments store raw data in formats that require specific (expensive...

...multiple exons that, when deleted through NHEJ, will restore the dystrophin reading frame. gRNAs bind in intronic..., Olson EN. Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular...

...Lung AAV4, AAV5, AAV6, AAV9 Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment... liquid nitrogen or a dry ice/ethanol bath, and store at -80° C. Once thawed, the AAV aliquot should be...

...using expired products or reagents that have been stored for a longer time. Use fresh media as much as possible...line to another. Multiple cell lines should not be stored in a single incubator since it increases the risk...

...         Adipocytes [28] Keratinocytes [29] Photoreceptors [30] Otic hair cells [31] Neurons [32, 33...Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem...

...photoswitchable proteins derived from the fungal photoreceptor, Vivid (VVD, N. crassa) (Kawano et al., 2015...single-chain fusion proteins by fusing Cas9 to photoreceptors. One prime example was from the lab of Michael...

...some of our other resources to help you choose, store, or use your antibody. Antibody Blog Posts Recipient...

...proteins are derived from bacterial phytochrome photoreceptors. Spectrally distinct fluorescent iRFP variants...